Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
By mobilizing small DNA units, integrons have a major function in the dissemination of antibiotic resistance among bacteria. The acquisition of gene cassettes occurs by recombination between the attI and attC sites catalysed by the IntI1 integron integrase. These recombination reactions use an unconventional mechanism involving a folded single-stranded attC site. We show that cellular bacterial processes delivering ssDNA, such as conjugation and replication, favour proper folding of the attC site. By developing a very sensitive in vivo assay, we also provide evidence that attC sites can recombine as cruciform structures by extrusion from double-stranded DNA. Moreover, we show an influence of DNA superhelicity on attC site extrusion in vitro and in vivo. We show that the proper folding of the attC site depends on both the propensity to form non-recombinogenic structures and the length of their variable terminal structures. These results draw the network of cell processes that regulate integron recombination.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2928681 | PMC |
| http://dx.doi.org/10.1038/emboj.2010.151 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Existence of structural, electronic and magnetic correlations in SmNiMnOdouble perovskite.
J Phys Condens Matter
February 2025
UGC DAE Consortium for Scientific Research, Indore 452001, India.
Coupling between different interactions allows the control of physical aspects in multifunctional materials by perturbing any degrees of freedom. Here, we aim to probe the correlation among structural, electronic, and magnetic observables in SmNiMnO(SNMO) ferromagnetic insulator double perovskite. Our employed methodology includes thermal evolution of x-ray diffraction, x-ray absorption spectroscopy, and bulk magnetometry.
View Article and Find Full Text PDFComparative genomics of IncQ1 plasmids carrying bla variants from clinical and environmental sources in Brazil.
Infect Genet Evol
September 2024
Faculdades Pequeno Príncipe, Curitiba, Paraná, Brazil; Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba, Paraná, Brazil.
IncQ-type plasmids have become important vectors in the dissemination of bla among different bacterial genera and species from different environments around the world, and studies estimating the occurrence of Guiana extended-spectrum (GES)-type β-lactamases are gaining prominence. We analyzed the genetic aspects of two IncQ1 plasmids harboring different bla variants from human and environmental sources. The bla variants were identified using polymerase chain reaction (PCR) in Aeromonas veronii isolated from hospital effluent and Klebsiella variicola isolated from a rectal swab of a patient admitted to the cardiovascular intensive care unit in a different hospital.
View Article and Find Full Text PDFScreening Key Sites of Class 2 Integron Integrase that Impact Recombination Efficiency.
Curr Microbiol
May 2024
Department of Laboratory Medicine, Anhui University of Science and Technology Affiliated Fengxian Hospital, 6600 Nanfeng Road, Shanghai, 201499, China.
By capturing and expressing exogenous resistance gene cassettes through site-specific recombination, integrons play important roles in the horizontal transfer of antimicrobial resistant genes among bacteria. The characteristics of integron integrase make it to be a potential gene editing tool enzyme. In this study, a random mutation library using error-prone PCR was constructed, and amino acid residues mutants that impact on attI2 × attC or attC × attC recombination efficiency were screened and analyzed.
View Article and Find Full Text PDFSimultaneous Enhancement of the Thermostability and Catalytic Activity of D-Allulose 3-Epimerase from ATTC BAA-613 Based on the "Back to Consensus Mutations" Hypothesis.
J Agric Food Chem
April 2024
College of Light Industry and Food Engineering, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China.
D-Allulose is a high value rare sugar with multiple physiological functions and commercial potential that can be enzymatically synthesized from D-fructose by D-allulose 3-epimerase (DAEase). Poor catalytic activity and thermostability of DAEase prevent the industrial production of D-allulose. In this work, rational design was applied to a previously identified DAEase from ATCC BAA-613 based on the "back to consensus mutations" hypothesis, and the catalytic activity of the Cb-I265 V variant was enhanced 2.
View Article and Find Full Text PDFIntegron cassettes integrate into bacterial genomes via widespread non-classical attG sites.
Nat Microbiol
January 2024
Institut Pasteur, Université Paris Cité, CNRS UMR 3525, Unité Plasticité du Génome Bactérien, Paris, France.
Integrons are genetic elements involved in bacterial adaptation which capture, shuffle and express genes encoding adaptive functions embedded in cassettes. These events are governed by the integron integrase through site-specific recombination between attC and attI integron sites. Using computational and molecular genetic approaches, here we demonstrate that the integrase also catalyses cassette integration into bacterial genomes outside of its known att sites.
View Article and Find Full Text PDF