Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Objective: To investigate the role of Ras antisense oligoribonucleotide (ASODN) in multidrug resistance (MDR) of pancreatic carcinoma Pc-2 cells.
Methods: Ras and P-gp expression was suppressed by Ras ASODN. Sensitivity of Pc-2 cells to chemotherapy was determined by the MTT assay. MDR-1 mRNA level was detected by fluorogenic probe quantitative reverse transcription polymerase chain (RT-PCR) method. Flow cytometry (FCM) was used to detect the accumulative concentration of adriamycin (ADR) in the cells.
Results: Ras ASODN significantly inhibited the Ras and P-gp expression (P < 0.05), increased the sensitivity of Pc-2 cells to chemotherapeutic agents (P < 0.05), decreased MDR-1 gene level in Pc-2 cells (P < 0.05), and increased the intracellular intake of ADR in Pc-2 cells (P < 0.05).
Conclusion: Ras ASODN may enhance the sensitivity of multidrug-resistant pancreatic cancer Pc-2 cells to chemotherapeutic agents by regulating MDR-1 gene level.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Recyclable luminescent solar concentrator from lead-free perovskite derivative.
Light Sci Appl
August 2025
Institute of Photoelectronic Thin Film Devices and Technology, Nankai University; Tianjin Key Laboratory of Efficient Utilization of Solar Energy, Nankai University; Engineering Research Center of Thin Film Photoelectronic Technology of Ministry of Education, Nankai University; State Key Laboratory
Luminescent solar concentrators (LSCs) offer a sustainable approach to power generation using fluorescent glasses, yet their green industrialization is impeded by the limited production scale and non-recyclability of embedded nanocrystals. Here, we introduce a lead-free perovskite derivative ETPSbCl (ETP = (CH)PCH) with a reversible transition between powder and glass states. Through molecular dynamics and density functional theory, we elucidate the possible structural distortions of [SbCl] pyramids and their impact on luminescence.
View Article and Find Full Text PDFApplication of STEM tomography to investigate smooth ER morphology under stress conditions.
J Microsc
September 2025
Department of Biophysics II/Structural Biology, University of Regensburg, Regensburg, Germany.
The endoplasmic reticulum (ER) is a highly dynamic organelle that undergoes significant morphological alterations in response to cellular stress. While conventional transmission electron microscopy (TEM) has provided valuable insights into these changes, such as the formation of crystalloid-ER and ER whorls, obtaining comprehensive three-dimensional (3D) information on these large structures within their cellular context has remained a challenge. To overcome these limitations, this study introduces an innovative application of dual-axis scanning transmission electron microscopy (STEM) tomography to investigate ER morphology under stress conditions in human embryonic kidney (HEK) cells overexpressing the cation channel polycystin-2 (PC-2).
View Article and Find Full Text PDFPotential zinc phthalocyanine-based photosensitizer for photodynamic therapy: Photophysical, theoretical and in vitro studies.
J Inorg Biochem
September 2025
Biomedical Technologies Application and Research Center (BIYOTAM), Sakarya University of Applied Sciences, Sakarya, Türkiye; Department of Engineering Fundamental Sciences, Sakarya University of Applied Sciences, Sakarya, Türkiye. Electronic address:
The preparation of new photosensitizers and studies on photodynamic therapy (PDT) have provided promising results and realistic expectations for an efficient medical treatment. Phthalocyanines (Pcs) have become extremely attractive for this purpose owing to their molecular versatility and superior physicochemical properties. Motivated by these facts, in this study, non-peripherally substituted zinc(II) Pc (2) was prepared by cyclotetramerization of the phthalonitrile derivative bearing 3-methoxybenzyloxy units.
View Article and Find Full Text PDFBiological signatures in the Alzheimer's continuum discriminate between diagnosis-related and -unrelated associations to ATN categories.
Brain Commun
February 2025
Division of Clinical Geriatrics, Department of Neurobiology, Care Sciences and Society, Karolinska Institutet, SE-171 77 Stockholm, Sweden.
Alzheimer's disease and related dementias have a multifactorial aetiology and heterogeneous biology. The current study aims to identify different biological signatures in a deeply phenotyped memory clinic patient population. In this cross-sectional study, we analysed 49 pre-specified proteins using a multiplex antibody-based suspension bead array in 278 CSF samples from the real-world research database and biobank at the Karolinska University Hospital Memory Clinic, Solna, Sweden.
View Article and Find Full Text PDFImmunome profiling in prostate cancer: a guide for clinicians.
Front Immunol
December 2024
Personalized Precision Medicine Chair, Universidad Autónoma de Madrid, Madrid, Spain.
Article Synopsis
- - Tumor immune microenvironment (TIME) significantly influences how prostate cancer (PC) responds to treatments and understanding mechanisms of resistance is crucial.
- - Research indicates that certain genomic changes, like microsatellite instability (MSI) and CDK12 bi-allelic loss, may increase response rates to immune therapies, but patient responses vary widely.
- - The review explores how immune cell interactions within tumors affect PC progression, how standard therapies impact immune responses, and the challenges in analyzing the immune landscape related to tumors.