Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The formation of the primitive endoderm covering the inner cell mass of early mouse embryos can be simulated in vitro by the differentiation of mouse embryonic stem (ES) cells in culture following either aggregation of suspended cells or stimulation of cell monolayers with retinoic acid. The developmentally regulated transcription factors GATA-4 and GATA-6 have determining role in mouse extraembryonic endoderm development. We analyzed the in vitro differentiation of mouse embryonic stem cells deficient of GATA factors and conclude that GATA-4 is required for ES cells to perceive a cell positioning (cell aggregation) signal and GATA-6 is required to sense morphogenic (retinoic acid) signal. The collaboration between GATA-6 and GATA-4, or GATA-6 and GATA-5 which can substitute for GATA-4, is involved in the perception of differentiation cues by embryonic stem cells in their determination of endoderm lineage. This study indicates that the lineage differentiation of ES cells can be manipulated by the expression of GATA factors.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ydbio.2005.07.037 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Molecular subtypes of human skeletal muscle in cancer cachexia.
Nature
September 2025
Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada.
Cancer-associated muscle wasting is associated with poor clinical outcomes, but its underlying biology is largely uncharted in humans. Unbiased analysis of the RNAome (coding and non-coding RNAs) with unsupervised clustering using integrative non-negative matrix factorization provides a means of identifying distinct molecular subtypes and was applied here to muscle of patients with colorectal or pancreatic cancer. Rectus abdominis biopsies from 84 patients were profiled using high-throughput next-generation sequencing.
View Article and Find Full Text PDFEngineering human neuronal diversity: Morphogens and stem cell technologies for neurodevelopmental biology.
Stem Cell Reports
September 2025
Child Study Center, Yale University, New Haven, CT 06520, USA; Program in Neurodevelopment and Regeneration, Yale University, New Haven, CT 06520, USA; Department of Neuroscience, Yale University, New Haven, CT 06520, USA; Yale Stem Cell Center, Yale University, New Haven, CT 06520, USA. Electronic
A complex assortment of neuronal cells contributes to distinct functional circuits in the human brain. Such diversity is imposed upon pluripotent stem cells by a patterning process that begins much before the start of neurogenesis. Neural tube patterning relies on morphogens-diffusible signals that regulate transcription factor networks in progenitor cells, guiding spatial and temporal identity formation.
View Article and Find Full Text PDFThree-dimensional co-culturing reveals human stem cell-derived somatostatin interneurons with subclass expression.
Stem Cell Reports
September 2025
Regenerative Neurophysiology, Lund Stem Cell Centre, MultiPark Strategic Area in Neuroscience, Department of Experimental Medical Science, Faculty of Medicine, Lund University, 221 84 Lund, Sweden. Electronic address:
Cortical interneuron deficiencies, particularly involving the somatostatin (SST) subtypes, contribute to neurological and neuropsychiatric disorders. These interneurons are difficult to derive in vitro from human embryonic stem cells (hESCs) due to their late embryonic development and dependence on glial interaction. To this end, we developed a three-dimensional co-culture model of hESC-derived neurons, enabling long-term development, functional maturity, and neuron-glial interaction.
View Article and Find Full Text PDFCorrigendum to "Human embryonic stem cell-derived cardiovascular progenitor cells stimulate cardiomyocyte cell cycle activity via activating the PI3K/Akt pathway" [J. Mol. Cell. Cardiol. 197 (2024) 5-10.].
J Mol Cell Cardiol
September 2025
CAS Key Laboratory of Tissue Microenvironment and Tumor, Laboratory of Molecular Cardiology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences (CAS), CAS, Shanghai 200031, PR China; Translational Medical Center for Stem Cell Therapy & Institutes of Heart Failure,
Bio-Scaffold Developed With Decellularized Human Amniotic Membrane Composite 3D Umbilical Cord Mesenchymal Stem Cell Spheroids Accelerate the Repair of Rat Defective Wounds.
FASEB J
September 2025
Department of Plastic Surgery and Burn, Third XiangYa Hospital, Central South University, Changsha, Hunan, China.
Defective wounds pose health risks, and treatment is challenging. Umbilical cord-derived mesenchymal stem cells (UCMSCs) show promise for healing. Primary UCMSCs were isolated and extracted in vitro, and the proliferation and differentiation characteristics were detected by flow cytometry and trilineage differentiation, and a 3D spherical cell culture was performed.
View Article and Find Full Text PDF