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The aim of this investigation was to establish whether Varroa destructor can play a role in the transmission of Paenibacillus larvae larvae spores from infected to healthy bee colonies. Mites, collected from an Apis mellifera carnica colony heavily infected with American foulbrood and treated with Apistan, were suspended in distilled water and treated in three different ways:homogenizing, shaking and stirring, or sonication. The resulting fluid samples were transferred onto selective agar medium. All culture plates showed colonies that could be identified as P.l. larvae. In view of the numbers of spores they can carry, it is concluded that mites may transmit American foulbrood from infected to healthy bee colonies.
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http://dx.doi.org/10.1023/a:1023392912999 | DOI Listing |
J Vet Diagn Invest
September 2025
Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
American foulbrood (AFB) and European foulbrood (EFB), caused by and , respectively, are severe bacterial diseases that significantly affect honey bee health and productivity worldwide. Rapid and accurate diagnosis is essential for effective disease management in apiaries. We developed and validated a multiplex point-of-care (POC) quantitative real-time PCR (qPCR) assay that enables simultaneous and rapid detection of and directly in apiaries.
View Article and Find Full Text PDFMicroorganisms
May 2025
Department of Experimental Biology, Faculty of Science, Masaryk University, 625 00 Brno, Czech Republic.
Host-specific and species constitute the core microbiota of the honey bee digestive tract and are recognized for their probiotic properties. One of the properties of these bacteria is the inhibition of bacterial pathogens such as and , the causative agents of American and European foulbrood, respectively. Additionally, has emerged as a relevant opportunistic pathogen.
View Article and Find Full Text PDFMicrobiol Spectr
July 2025
Department of Biology, The University of Western Ontario, London, Ontario, Canada.
Beneficial bacteria can improve the health of managed honey bees () via immune system support as well as direct inhibition of pathogens. However, our understanding of how environmental factors and delivery methods impact treatment outcomes is limited. Here, we evaluated how supplementation of a three-strain lactobacilli consortium ( Lp39, BR-1, and ; LX3) affects ectoparasitic mite () and bacterial () load.
View Article and Find Full Text PDFEnviron Microbiol Rep
June 2025
Swiss Bee Research Centre, Agroscope, Bern, Switzerland.
Honey bee colonies contain thousands of individuals living in close proximity in a thermally homeostatic nest, creating ideal conditions for the thriving of numerous pathogens. Among the bacterial pathogens, Paenibacillus larvae infects larvae via the nutritive jelly that adult workers feed them, causing the highly contagious American foulbrood disease. Further Paenibacillus species were anecdotally found in association with honey bees, including when affected by another disease, European foulbrood (EFB).
View Article and Find Full Text PDFMicrob Genom
May 2025
NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Woodbridge Rd, Menangle, NSW, Australia.
is the causative agent of American foulbrood (AFB) in honeybees () and a devastating pathogen for honey and pollination industries worldwide. Despite this threat, a genomic survey of has not been attempted within Australia. To examine the diversity of Australian populations, we sequenced 368 .
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