PEG-Mediated Protoplast Transformation of (scaumcx01): Metabolomic Shifts and Root Colonization Dynamics.

Protoplast-based transformation is a vital tool for genetic studies in fungi, yet no protoplast method existed for -scaumcx01 before this study. Here, we optimized protoplast isolation, regeneration, and transformation efficiency. The highest protoplast yield (6.

Influence of Kunth Flavonoids on Composition of Soil Microbial Community.

, one of the world's most destructive invasive species, is known for causing significant ecological and economic harm. While extensive research has focused on its growth characteristics, secondary metabolites, and control measures, its chemical interactions with the environment-particularly the role of flavonoids in shaping soil microbial communities-remain underexplored. In this study, we identified and quantified ten flavonoids from root exudates using UPLC-MS, including Hispidulin, Isorhamnetin, and Mikanin.

Hydrophobic association supramolecular gel suitable for oil and gas drilling in fractured formation.

Supramolecular gel can be used to seal fractures and pores in the formation during oil and gas drilling and production. In this study, a supramolecular gel plugging agent based on hydrophobic association was prepared by free radical polymerization of acrylamide, octadecyl methacrylate, sodium dodecyl sulfate and other monomers by micellar copolymerization. The forming time, rheology, swelling, mechanical properties and plugging properties of supramolecular gels were studied.

Numerical Analysis of the Effect of Retaining Ring Structure on the Chemical Mechanical Polishing Abrasive Motion State.

Optimizing the retaining ring structure can improve the quality of Chemical Mechanical Polishing (CMP). This study establishes a two-dimensional Computational Fluid Dynamics-Discrete Element Method (CFD-DEM) model, while the model is validated by experiments. The results graphically demonstrate the influence of the retaining ring groove design on the motion of the slurry abrasive particles.