Construction of chitin nanocrystal/poly(deep eutectic solvent) elastomers via glycidyl methacrylate associated modification, polymerization and crosslinking for artificial skin applications.

A one-pot strategy was developed to fabricate a strong and ductile elastomer composed of chitin nanocrystals and poly(deep eutectic solvent) (ChNC/PDES), based on a dual-network structure formed through glycidyl methacrylate (GMA)associated modification, polymerization and crosslinking. This approach enables the integrated pretreatment, chemical modification, and nanodispersion of chitin within a lactic acid/choline chloride deep eutectic solvent (DES) system. Whereafter, the ultraviolet initiated polymerization of GMA with ChNC and DES components produced a homogeneous elastomer with a maximum tensile strength of 4.

Preparation of Janus film for fog water collection via layer-by-layer assembling of nanocellulose and nanochitin on PLA.

In order to explore the possibility of natural carbohydrate polymers as a biodegradable and sustainable fog water harvesting material, this work proposed an efficient substrate (hydrophobic)-transition layer (amphoteric)-coating (hydrophilic) sandwich spin-coating strategy to form all biomass-based Janus film. The oxalic acid hydrolyzed nanochitin (OAChN) was applied as a transition layer that enabled successful spin-coating of the hydrophilic nanocellulose (TEMPO-oxidized cellulose nanofiber, TOCN) and nanochitin (partially deacetylated chitin nanofibers, DEChN) on the hydrophobic polylactic acid (PLA) film substrate. In which a layer-by-layer (LBL) assembling of TOCN (carboxyl-rich negative surface charge) and DEChN (amino-rich positive surface charge) was designed to form a thickness and surface property controllable polysaccharide coating on PLA.

High internal phase Pickering emulsions stabilized by ε-poly-l-lysine grafted cellulose nanofiber for extrusion 3D printing.

An effective method for preparing food-grade three-dimensional (3D) printing materials was the use of highly concentrated oil-in-water emulsions. This research reported 3D printable materials constructed from food-grade high internal phase Pickering emulsions (HIPPEs) that were stabilized by ε-poly-l-lysine grafted cellulose nanofiber (ε-PL-TOCNs). The ε-PL-TOCNs were prepared via ε-poly-l-lysine grafting of 2, 2, 6, 6-tetramethylpiperidine-N-oxyl (TEMPO)-oxidized cellulose (TOC) and the successive mechanical treatment.

Comparison of cellulose and chitin nanofibers on Pickering emulsion stability-Investigation of size and surface wettability contribution.

There is an increasing concern about developing biobased colloid particles for Pickering stabilization due to the environment-friendliness and health-safety needs. In this study, Pickering emulsions were formed by using TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl radical)-mediated oxidized cellulose nanofibers (TOCN) and chitin nanofibers prepared by TEMPO-mediated oxidation (TOChN) or partial deacetylation (DEChN). The physicochemical characterizations of Pickering emulsions demonstrated that the higher cellulose or chitin nanofiber concentrations, surface wettability, and zeta-potential, the higher effectiveness in Pickering stabilization.

Core antigenic advantage domain-based ELISA to detect antibody against novel goose astrovirus in breeding geese.

Goose astrovirus (GAstV), the major causative agent of visceral and joint gout in goslings, is a novel pathogen greatly threatening waterfowl industry. Importantly, the horizontal and vertical transmissibility of GAstV posed a great challenge for disease prevention and control. Given the absence of commercial vaccine, restricting vertical transmission and protecting susceptible goslings must be a priority.

Development and application of SYBR Green Ⅰ real-time quantitative reverse transcription PCR assay for detection of swine Getah virus.

Getah virus (GETV), a mosquito-borne virus belonging to the Alphavirus genus of family Togaviridae, has become increasingly problematic, which poses a huge threat to the safety of animals and public health. In order to detect GETV quickly and accurately, we have developed a SYBR Green I real-time quantitative reverse transcription PCR (RT-qPCR) assay for GETV with the detection limit of 66 copies/μL, excellent correlation coefficient (R) of 0.9975, and amplification efficiency (E) of 98.