Combining a novel ensemble model and multiplex methylation SNaPshot assays for saliva age prediction and cross-platform data analysis.

Background: DNA methylation is a pivotal biomarker for age prediction. However, most studies focus on blood-derived data, with limited research on saliva, and the inability to directly analyze methylation data across diverse platforms constrains predictive accuracy.

Results: We identified 10 age-related CpG sites in saliva (cg00481951, cg07547549, cg10501210, cg13654588, cg14361627, cg15480367, cg17110586, cg17885226, cg19671120, cg21296230) through six Illumina HumanMethylation450 BeadChip datasets and developed two multiplex SNaPshot assays.

Procalcitonin and C-reactive protein as early predictors in patients at high risk of colorectal anastomotic leakage.

Objective: To assess the diagnostic value of C-reactive protein (CRP) and procalcitonin (PCT) for anastomotic leakage (AL) following colorectal surgery.

Methods: We retrospectively analyzed data for patients who underwent colorectal surgery at our hospital between November 2019 and December 2023. CRP and PCT were measured postoperatively to compare patients with/without AL, and changes were compared between low- and high-risk groups.

A portable and miniaturized lab-on-fiber sensor based on a responsive Fabry-Perot resonance cavity for the detection of thiocyanate.

Thiocyanate (SCN) detection is highly significant because of the toxicity of SCN. Herein, a portable and miniaturized lab-on-fiber (LOF) sensor is reported for the detection of SCN through integrating a Fabry-Perot (F-P) optical resonance cavity based on anionic-responsive metal-insulator-metal (MIM) onto an optical fiber tip. The responsive MIM optical resonance cavity is constructed with an intermediate cationic polymer brush layer (poly[2-(methacryloyloxy)ethyl] trimethylammonium chloride, PMETAC) and two silver layers a facile "layer-by-layer" construction method.

Mechanism of Mongolian Medicine in Improving Postoperative Cognitive Dysfunction Through Activation of the PI3K Signaling Pathway.

Objective: To study the effect of , a traditional Mongolian medicine, on the cognitive function of rats by activating the IRS-PI3K-AKT-GLUT4 pathway in an animal model of postoperative cognitive dysfunction (POCD).

Methods: Fifty clean-grade adults Sprague Dawley (SD) male rats were assigned to one of five groups: (1) a control group with no anesthesia (Group C), (2) a POCD model group with anesthesia only (Group P), (3) POCD group with low-dose EW treated (Group L), (4) a POCD group with high-dose EW treated (Group H), and (5) a POCD model group with dexmedetomidine treated (Group D) for positive control. The study started 7 days after all rats had acclimated to housing.

Danon disease: Two patients with atrial fibrillation in a single family and review of the literature.

The present study reports on a family with two members affected by Danon disease but having different phenotypes. The clinical manifestations of Danon disease include cardiomyopathy, skeletal myopathy and different degrees of intellectual disability that varies greatly among patients. The present case study reports on two siblings, an older sister and a younger brother, with Danon disease from an affected pedigree, presenting with distinctly different phenotypes.

Interaction between bacterial outer membrane proteins and periplasmic quality control factors: a kinetic partitioning mechanism.

The OMPs (outer membrane proteins) of Gram-negative bacteria have to be translocated through the periplasmic space before reaching their final destination. The aqueous environment of the periplasmic space and high permeability of the outer membrane engender such a translocation process inevitably challenging. In Escherichia coli, although SurA, Skp and DegP have been identified to function in translocating OMPs across the periplasm, their precise roles and their relationship remain to be elucidated.