[Protective effect of breviscapine against brain injury induced by intrauterine inflammation in preterm rats and its mechanism].

Objectives: To study the protective effect of breviscapine against brain injury induced by intrauterine inflammation in preterm rats and its mechanism.

Methods: A preterm rat model of brain injury caused by intrauterine inflammation was prepared by intraperitoneal injections of lipopolysaccharide in pregnant rats. The pregnant rats and preterm rats were respectively randomly divided into 5 groups: control, model, low-dose breviscapine (45 mg/kg), high-dose breviscapine (90 mg/kg), and high-dose breviscapine (90 mg/kg)+ML385 [a nuclear factor erythroid 2-related factor 2 (Nrf2) inhibitor, 30 mg/kg] (=10 each).

Prevalence and Genetic Analysis of β-Thalassemia in the Dali Bai Autonomous Prefecture of the Yunnan Province, China.

β-Thalassemia is the most common monogenetic hemolytic hemoglobin-associated disease in the south of China; the distribution of genetic mutations associated with this condition varies according to geographic regions. This study investigated the prevalence and distribution of β-thalassemia-associated mutations across different ethnic groups in the Dali Bai Autonomous Prefecture of the Yunnan Province, China. This cross-sectional study included 4723 participants (15-45 years old) who volunteered for thalassaemia screening from the Dali Bai Autonomous Prefecture from May 2017 to October 2020.

The antimicrobial photodynamic inactivation resistance of Candida albicans is modulated by the Hog1 pathway and the Cap1 transcription factor.

Candida albicans is the most important fungal pathogen afflicting humans, particularly immunocompromised patients. However, currently available antifungal drugs are limited and ineffective against drug-resistant strains. The development of new drugs or alternative therapeutic approaches to control fungal infections is urgent and necessary.

[Application of two auricular cartilage-perichondrium complexes in tympanoplasty].

Objective: To compare the effects of cartilage-perichondrium palisade complex and cartilage-perichondrium horseshoe complex in tympanoplasty for large tympanic membrane perforation.

Methods: Nineteen patients (19 ears) undergoing tympanoplasty with cartilage-perichondrium palisade complex and 20 patients (20 ears) with cartilage-perichondrium horseshoe complex were compared for postoperative hearing and closure rate of tympanic membrane perforation.

Results: The closure rates of the tympanic membrane were all 100% in both groups 3 months after the operation, while tympanoplasty with cartilage- perichondrium horseshoe complex resulted in significantly greater improvement of the postoperative air-bone gap in speech frequency.

Voltammetric Behavior of o-Nitrophenol and Damage to DNA.

The electrochemical behavior of o-nitrophenol was studied in detail with a glassy carbon electrode (GCE). The dependence of peak potential on pH indicated that equivalent electrons and protons were involved in the process of o-nitrophenol reduction. The interaction of o-nitrophenol with calf thymus DNA was investigated by adding DNA to the o-nitrophenol solution and by immobilizing DNA on GCE, respectively.

An amperometric horseradish peroxidase inhibition biosensor for the determination of phenylhydrazine.

An amperometric horseradish peroxidase (HRP) inhibition biosensor has been substantially constructed by the help of N,N-dicyclohexylcarbodiimide (DCC), N-hydroxysuccinimide (NHS). The preparation steps and the biosensor response to phenylhydrazine were monitored by electrochemical impedance spectroscopy (EIS), cyclic voltammetry, and chronoamperometry. The proposed biosensor could be applied to determine phenylhydrazine in a 0.

Electrochemical determination of trace promethazine hydrochloride by a pretreated glassy carbon electrode modified with DNA.

A highly sensitive electrochemical biosensor for the detection of trace amounts of promethazine has been designed. Double stranded (ds)DNA molecules are immobilized onto a pretreated glassy carbon electrode (GCE(ox)) surface. The voltammetric behaviors of promethazine on DNA-modified electrode were explored by means of cyclic voltammetry (CV) and differential pulse voltammetry (DPV).

Feeding scallop shell powder induces the expression of uncoupling protein 1 (UCP1) in white adipose tissue of rats.

Previously we found that the organic components in scallop shell promote lipolysis in differentiated 3T3-L1 and C3H10T1/2 adipocyte cells, and that incorporating scallop shell powder into the diet of rats reduced the amount of white adipose tissue. In this study, we used RT-PCR to investigate the effect of ingesting scallop shell powder on the gene expression profile of uncoupling proteins (UCPs) regulating energy metabolism in rats. Feeding of scallop shell powder increased mRNA levels of UCP1 and UCP2 in white adipose tissue.

Direct electrochemical behavior of cytochrome c on DNA-modified glassy carbon electrode and its application to cytochrome c biosensor.

DNA was immobilized on glassy carbon electrodes to fabricate DNA-modified electrodes. The direct electron transfer of horse heart cytochrome c on DNA-modified glassy carbon electrode was achieved. A pair of well-defined redox peaks of cytochrome c appeared at Epc = -0.

Direct electrochemistry behavior of cytochrome c/L-cysteine modified electrode and its electrocatalytic oxidation to nitric oxide.

Cytochrome c (Cyt c) was successfully immobilized on L-cysteine modified gold electrode by multicyclic voltammetry method. The electrochemical behavior of Cyt c on the L-cysteine modified electrode was explored. In 0.

Reducing effect of feeding powdered scallop shell on the body fat mass of rats.

The lipolytic effect of powdered scallop shells was estimated in vitro and in vivo. The scallop shells consisted of 98% calcium carbonate and 2% organic compounds, the extracted organic components promoted lipolysis in 3T3-L1 adipocyte cells. Male Wistar rats were fed on an experimental diet containing either the scallop shell powder or calcium carbonate (control) for 28 d.

Potential-modulated DNA cleavage by (N-salicylideneglycinato)copper(II) complex.

The interaction of aqua (N-salicylideneglycinato)copper(II) (Cu(salgly)2+) complex with calf thymus DNA has been investigated by cyclic voltammetry. Potential-modulated DNA cleavage in the presence of Cu(salgly)2+ complex was performed at a gold electrode in a thin layer cell. DNA can be efficiently cleaved by electrochemically reducing Cu(salgly)2+ complex to Cu(salgly)+ complex at -0.

Electrochemical cleavage of DNA in the presence of copper-sulfosalicylic acid complex.

Electrochemical cleavage of DNA in the presence of copper-sulfosalicylic acid [Cu(ssal)(2)(2+)] complex was studied. The cleavage was observed in a certain potential region where redox cycling of Cu(ssal)(2)(2+)/Cu(ssal)(2)(+) took place. Cu(ssal)(2)(2+) complex mediate generation of reactive oxygen species from O(2) by the Fenton reaction, these radicals are capable of damaging DNA.