Evaluation of Modified CC Stabilization using LARS Artificial Ligament in Unstable Distal Clavicle Fracture.

Unstable distal clavicle fractures are clinically common, and there is no consensus regarding gold-standard treatment. The purpose of this study was to report on a new surgical technique for the treatment of unstable distal clavicle fracture with modified coracoclavicular (CC) stabilization using a ligament augmentation and reconstruction system (LARS) of an artificial ligament, and to compare the clinical and radiographic outcomes with hook-plate fixation.Thirty patients with unstable distal clavicle fractures were treated with modified coracoclavicular (CC) stabilization using either a ligament augmentation and reconstruction system (LARS) or open reduction internal fixation with a hook plate.

Anterior lumbar‒iliac fixation for unstable pelvic fractures: a novel surgical technique.

The treatment of unstable pelvic fractures is a great challenge for surgeons. Traditional steel plate fixation and sacroiliac screw fixation are not sufficient to provide longitudinal support. At present, surgical lumbar‒iliac fixation is a reliable fixation technique.

SUR1-TRPM4 Regulates Aquaporin-4 Subcellular Localization by Astrocytic Endfeet Calcium Signals Following Status Epilepticus.

Status epilepticus (SE) is a severe condition that results in uncontrollable cerebral edema and cognitive dysfunction. Recent studies suggest that the localization of aquaporin-4 (AQP4) in astrocytic endfeet plays a crucial role in regulating blood-brain water transport and cell volume control, particularly along perivascular pathways. However, the signaling mechanisms underlying AQP4 localization remain poorly understood.

Quasi-Scholte wave-based acoustofluidics: Trapping, levitation, and movement of microparticles.

Conventional acoustofluidic devices typically utilize high-frequency surface acoustic waves or Lamb waves to manipulate particles in fluid. However, since these waves possess a supersonic phase velocity (i.e.

Advancements in the identification and utilization of cerebrospinal fluid immunological biomarkers for the diagnosis of tuberculous meningitis.

Tuberculous meningitis (TBM), the most severe manifestation of tuberculosis, poses a major global health challenge due to its high morbidity and mortality, affecting over 100,000 individuals annually. Diagnosing TBM is challenging due to its clinical resemblance to other forms of meningitis and the slow, often inadequate, traditional diagnostic methods like CSF smear and culture. Despite advancements such as the Xpert MTB/RIF assay, which has enhanced the speed and sensitivity of diagnostics, significant challenges persist due to variability in test performance and sample handling.

Targeting TRPV4 to restore glymphatic system function and alleviate cerebral edema in ischemic stroke.

Emerging studies underscore the pivotal role of glymphatic system (GS) dysfunction in the pathogenesis of cerebral edema following brain injury. The transient receptor potential vanilloid 4 (TRPV4) channels have been implicated in modulating the polarization of aquaporin-4 (AQP4), a key protein involved in GS function. This study investigates the potential of targeting TRPV4 to alleviate GS dysfunction and reduce cerebral edema following ischemic stroke.

Future research focus and trends in nerve transplantation: a bibliometrics research from 2015 to 2024.

Background: Nerve transplantation, as a classical nerve repair technique, has received extensive attention in recent years. However, the rapid development of this field has also brought challenges such as knowledge fragmentation and the blurring of research hotspots. Therefore, at present, the future research direction of this field is not clear.

Biocompatibility and therapeutic efficacy of crosslinked hydrogel filled 3D-printed nerve conduit for sacral nerve injury repair.

Neurological system injuries are debilitating conditions that significantly impact patients' quality of life. This study investigated using a polycaprolactone (PCL) nerve conduit loaded with anti-epidermal growth factor receptor (EGFR) hydrogel and neural stem cells (NSCs) for treating sacral nerve injury (SNI) in rats to explore its neural repair effects. The results demonstrate that the combined transplantation therapy using a 3D printed scaffold filled with crosslinked hydrogel and NSCs effectively improves SNI, with the PCL Nerve conduit showing potential promotion of neuronal differentiation.

A structural equation modeling approach to investigating the influence of smart kitchen appliance design features on older adults' technology acceptance.

This study investigates the influence of smart kitchen appliance design features on older adults' technology acceptance in China using the Smart Kitchen Appliance-Senior Technology Acceptance Model (SKA-STAM). The SKA-STAM extends the Technology Acceptance Model (TAM) with five key design elements: ergonomics, visuals, functions, interactive interfaces, and brands. A cross-sectional survey using a structured questionnaire was administered to 352 Chinese older adults aged 65-84 in first- and second-tier cities from August to October 2024.

Potentiating microglial efferocytosis by MFG-E8 improves survival and neurological outcome after successful cardiopulmonary resuscitation in mice.

Brain injury represents the leading cause of mortality and disability after cardiopulmonary resuscitation (CPR) from cardiac arrest (CA), in which the accumulation of dying cells aggravate tissue injury by releasing proinflammatory intracellular components. Microglia play an essential role in maintaining brain homeostasis via milk fat globule epidermal growth factor 8 (MFG-E8)-opsonized efferocytosis, the engulfment of dying cells and debris. This study investigates whether potentiating microglia efferocytosis by MFG-E8 provides neuroprotection after CA/CPR.

Suppression of KLF5 targets RREB1 to restrain the proliferation of ovarian cancer cells through ERK/MAPK signaling pathway.

The overexpression of Kruppel-like factor 5 (KLF5) appears in several types of cancer. KLF5 may be an effective therapeutic target for treating OC, but its function in ovarian cancer (OC) remains unknown. The KLF5 mRNA expression levels in several OC cell lines were analyzed using RT-qPCR.

New mechanistic understanding of osteoclast differentiation and bone resorption mediated by P2X7 receptors and PI3K-Akt-GSK3β signaling.

Objective: Osteoporosis is a global health issue characterized by decreased bone mass and microstructural degradation, leading to an increased risk of fractures. This study aims to explore the molecular mechanism by which P2X7 receptors influence osteoclast formation and bone resorption through the PI3K-Akt-GSK3β signaling pathway.

Methods: An osteoporosis mouse model was generated through ovariectomy (OVX) in normal C57BL/6 and P2X7; LysM-cre mice.

Covalent organic framework based cytoprotective therapy after ischemic stroke.

Cytoprotection has emerged as an effective therapeutic strategy for mitigating brain injury following acute ischemic stroke (AIS). The sulfonylurea receptor 1-transient receptor potential M4 (SUR1-TRPM4) channel plays a pivotal role in brain edema and neuroinflammation. However, the practical use of the inhibitor glyburide (GLB) is hindered by its low bioavailability.

Evaluation of tumor microvasculature with 3D ultrasound localization microscopy based on 2D matrix array.

Objective: Evaluation of tumor microvascular morphology is of great significance in tumor diagnosis, therapeutic effect prediction, and surgical planning. Recently, two-dimensional ultrasound localization microscopy (2DULM) has demonstrated its superiority in the field of microvascular imaging. However, it suffers from planar dependence and is unintuitive.

Skin-Conformable Flexible and Stretchable Ultrasound Transducer for Wearable Imaging.

Ultrasound imaging offers a noninvasive, radiation-free method for visualizing internal tissues and organs, with deep penetration capabilities. This has established it as a crucial tool for physicians in diagnosing internal tissue pathologies and monitoring human conditions. Nonetheless, conventional ultrasound probes are often characterized by their rigidity and bulkiness.

Identification of acetylshikonin as a novel tubulin polymerization inhibitor with antitumor activity in human hepatocellular carcinoma cells.

Background: Microtubules are attractive targets for anticancer drugs. However, the microtubule-targeting agents (MTAs) currently in clinical use exhibit inevitable drug resistance. Therefore, there is an urgent need to discover novel MTAs for the clinical treatment of cancer.

MicroRNA-584-5p/RUNX family transcription factor 2 axis mediates hypoxia-induced osteogenic differentiation of periosteal stem cells.

Background: The hypoxic environment during bone healing is important in regulating the differentiation of periosteal stem cells (PSCs) into osteoblasts or chondrocytes; however, the underlying mechanisms remain unclear.

Aim: To determine the effect of hypoxia on PSCs, and the expression of microRNA-584-5p (miR-584-5p) and RUNX family transcription factor 2 (RUNX2) in PSCs was modulated to explore the impact of the miR-584-5p/RUNX2 axis on hypoxia-induced osteogenic differentiation of PSCs.

Methods: In this study, we isolated primary mouse PSCs and stimulated them with hypoxia, and the characteristics and functional genes related to PSC osteogenic differentiation were assessed.

Chemical Constituents from Mycelia of Lepista sordida (Agaricomycetes) and Their ABTS Radical Scavenging Activity.

Lepista sordida is an edible mushroom possessing high nutritional value and high medicinal value. The artificial cultivation technology of L. sordida made a breakthrough and has been popularized in Yunnan, Guizhou, Sichuan province with good economic benefits.

In-vivo programmable acoustic manipulation of genetically engineered bacteria.

Acoustic tweezers can control target movement through the momentum interaction between an acoustic wave and an object. This technology has advantages over optical tweezers for in-vivo cell manipulation due to its high tissue penetrability and strong acoustic radiation force. However, normal cells are difficult to acoustically manipulate because of their small size and the similarity between their acoustic impedance and that of the medium.

In vivo ocular microvasculature imaging in rabbits with 3D ultrasound localization microscopy.

Morphological and hemodynamic changes in the ocular vasculature are important signs of various ocular diseases. The evaluation of the ocular microvasculature with high resolution is valuable in comprehensive diagnoses. However, it is difficult for current optical imaging techniques to visualize the posterior segment and retrobulbar microvasculature due to the limited penetration depth of light, particularly when the refractive medium is opaque.

Flexible Ultrasound Transducer With Embedded Optical Shape Sensing Fiber for Biomedical Imaging Applications.

Flexible ultrasound transducers (FUTs), capable of conforming to irregular surfaces, have become a research hotspot in the field of medical imaging. With these transducers, high-quality ultrasound images can be obtained only if strict design criteria are fulfilled. Moreover, the relative positions of array elements must be determined, which are important for ultrasound beamforming and image reconstruction.

QFD-Based Research on Sustainable User Experience Optimization Design of Smart Home Products for the Elderly: A Case Study of Smart Refrigerators.

In the current situation of global aging, the current market shortage of age-appropriate smart home products and the recent epidemic have led to greater isolation of the elderly, seriously affecting their physical and mental health. In order to optimize the sustainable user experience of the elderly when using smart home products, this paper proposes a research method based on Quality Function Deployment (QFD) for the optimal design of user experience of smart home products for the elderly, taking the design of age-appropriate home smart refrigerators as an example. Firstly, based on the results of market research and user interviews, the requirements of smart refrigerators for the elderly are screened and integrated, and the Kano model is used to prioritize these needs, resulting in the identification of important features needed in smart refrigerators for the elderly.

LINC00665 affects the malignant biological behavior of ovarian cancer via the miR-148b-3p/KLF5.

This study investigated the expression and clinical significance of long intergenic noncoding RNA 00665 (LINC00665) in ovarian cancer (OC), as well as its effect on the malignant biological behavior of OC cells. The expression of LINC00665, miR-148b-3p, and Krüppel-like factor 5 (KLF5) in OC tissues and cells were determined by RT-qPCR. Western blot was used to detect the protein expression of KLF5.

Endoscopic Ultrasound Localization Microscopy for the Evaluation of the Microvasculature of Gastrointestinal Tract Tumors in Rabbits.

Objective: The morphological and hemodynamic characterization of the microvascular network around the gastrointestinal (GI) tract can be of significant clinical value for the early diagnosis and treatment of GI tract cancer. Ultrasound localization microscopy (ULM) imaging has been demonstrated to be capable of resolving the microvascular network. However, the endoscopic application of ULM imaging techniques is still unknown.