Identification of small compounds targeting ferroptosis in triple-negative breast cancer by using an integrated computational approach.

This study integrated bioinformatics and computational approaches to identify potential therapeutic compounds targeting ferroptosis in triple-negative breast cancer (TNBC) patients. Through analysis of TNBC transcriptomic data from the GEO and FerrDb databases, we identified 140 TNBC-associated ferroptosis genes. There were seven hub genes (KRAS, PKM, MAPK1, AR, AURKA, TFRC and ESR1), which emerged as potential diagnostic biomarkers.

The use of polyphenols extracted from Chinese sweet leaf tea (Rubus suavissimus S. Lee.) as novel drugs for the treatment of metabolic dysfunction associated steatotic liver disease.

Background: Metabolic dysfunction associated steatotic liver disease (MASLD) is a pressing global health issue with limited treatment options. Chinese sweet leaf tea (CSLT), rich in polyphenols, shows promise in addressing MASLD due to its anti-inflammatory, obesity-reducing, and metabolic-regulating properties. This study aimed to explore the therapeutic potential of CSLT polyphenols for MASLD treatment.

Chemical and Mechanistic Prediction Analysis of Anti-obesity Properties of Guang Hawthorn (Malus doumeri) Leaves using Network Analysis.

Objective: This study aimed to characterize the bioactive compounds in Guang hawthorn (Malus doumeri) leaf extracts and evaluate their potential anti-obesity effects.

Methods: 70% Ethanol and ethyl acetate extracts from M. doumeri leaves were administered to high-fat diet-induced obese rats.

Ultra-High-Performance Liquid Chromatography-High-Definition Mass Spectrometry-Based Metabolomics to Reveal the Potential Anti-Arthritic Effects of in Cultured Fibroblast-like Synoviocytes Derived from Rheumatoid Arthritis.

Background: Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease. The fruits of , which is a medicinal and edible resource, have been shown to have anti-inflammatory properties.

Methods: In this study, we investigated the effects of extracts (IVEs) on human RA fibroblasts-like synoviocytes (RA-FLS) by using a sensitive and selective ultra-high-performance liquid chromatography with high-definition mass spectrometry (UPLC-HDMS) method.

Molecular mechanism of regulating tat protein expression of pingganjiedu TCM in the treatment of AIDS based on network pharmacology.

AIDS is a serious disease with impaired immune function caused by human immunodeficiency virus (HIV) infection. The treatment of AIDS has always been the focus of global scientific research, and Tat protein is a key regulatory protein in the process of HIV infection. Its high expression is closely related to virus replication, disease progression, etc.

Knockdown of Anoikis-Associated Gene OCIAD2 Reduces Proliferation and Migration of Glioblastoma Cell Lines.

Background: Glioblastoma (GBM) severely disrupts the quality of life of patients. Anoikis represents a significant mechanism in cancer invasion and metastasis. Our study focused on the prognostic relationship between the anoikis-associated gene and GBM and its effect on GBM cell progression.

Explorative Study on Volatile Organic Compounds of Cinnamon Based on GC-IMS.

Cinnamon is one of the most popular spices worldwide, and volatile organic compounds (VOCs) are its main metabolic products. The misuse or mixing of cinnamon on the market is quite serious. This study used gas chromatography-ion migration spectroscopy (GC-IMS) technology to analyze the VOCs of cinnamon samples.

The complete chloroplast genome sequence of Thunb.

(Thunb.) A. DC.

The complete chloroplast genome sequence of Osbeck.

Osbeck is an important medicinal and edible plant. The complete chloroplast genome of Osbeck was constructed and annotated for the first time in this study. Full length of the chloroplast genome of Osbeck is 159,647 bp, with a large single-copy (LSC) region of 86,301 bp, a small single-copy (SSC) region of 79,536 bp, and a pair of inverted repeats IRa and IRb regions of 31,039 bp.

The complete chloroplast genome of Vahl.

Vahl. belongs to the family Lamiaceae. Its root is a widely used Yao Medicine (YM) to treat internal and external bleeding at the Yao minority areas in southern China.

Phylogenetic analysis and development of molecular markers for five medicinal Alpinia species based on complete plastome sequences.

Background: Alpinia species are widely used as medicinal herbs. To understand the taxonomic classification and plastome evolution of the medicinal Alpinia species and correctly identify medicinal products derived from Alpinia species, we systematically analyzed the plastome sequences from five Alpinia species. Four of the Alpinia species: Alpinia galanga (L.

Comparative genome analysis revealed gene inversions, boundary expansions and contractions, and gene loss in the Stemona sessilifolia (Miq.) Miq. chloroplast genome.

Stemona sessilifolia (Miq.) Miq., commonly known as Baibu, is one of the most popular herbal medicines in Asia.

Peculiarities of the inverted repeats in the complete chloroplast genome of Lindau.

Lindau belongs to the family It is an antiviral herb that can be used to prevent Influenza virus infections in the border areas between China and Vietnam. Local people call it 'Purple Ban-lan-gen' because its root is very similar to that of (Nees) Kuntze, which is called 'Southern Ban-lan-gen' and is listed in Chinese Pharmacopeia. The two species have been used interchangeably locally.

Sequencing and Analysis of (Nees) Kuntze Chloroplast Genome Revealed the Rare Simultaneous Contraction and Expansion of the Inverted Repeat Region in Angiosperm.

Ban-Lan-Gen, the root tissues derived from several morphologically indistinguishable plant species, have been used widely in traditional Chinese medicines for numerous years. The identification of reliable markers to distinguish various source plant species is critical for the effective and safe use of products containing Ban-Lan-Gen. Here, we analyzed and characterized the complete chloroplast (cp) genome sequence of (Nees) Kuntze to identify high-resolution markers for the species determination of Southern Ban-Lan-Gen.

Complete plastid genome of (Fisch.) Bunge var. membranaceus.

The complete nucleotide sequence of the (Fisch.) Bunge var. membranaceus chloroplast genome was reported and characterized in this study.

Hemagglutinin-based polyanhydride nanovaccines against H5N1 influenza elicit protective virus neutralizing titers and cell-mediated immunity.

H5N1 avian influenza is a significant global concern with the potential to become the next pandemic threat. Recombinant subunit vaccines are an attractive alternative for pandemic vaccines compared to traditional vaccine technologies. In particular, polyanhydride nanoparticles encapsulating subunit proteins have been shown to enhance humoral and cell-mediated immunity and provide protection upon lethal challenge.

Structural and antigenic stability of H5N1 hemagglutinin trimer upon release from polyanhydride nanoparticles.

Although H5N1 avian influenza has not yet acquired the capacity to readily infect humans, should it do so, this viral pathogen would present an increasing threat to the immunologically naïve human population. Subunit vaccines based on the viral glycoprotein hemagglutinin (HA) can provide protective immunity against influenza. Polyanhydride nanoparticles have been shown to enhance efficacy of subunit vaccines, providing the dual advantages of adjuvanticity and sustained delivery resulting in enhanced protein stability and immunogenicity.

Photoswitching-induced frequency-locked donor-acceptor fluorescence double modulations identify the target analyte in complex environments.

Precisely identifying biological targets and accurately extracting their relatively weak signals from complicated physiological environments represent daunting challenges in biological detection and biomedical diagnosis. Fluorescence techniques have become the method of choice and offer minimally invasive and ultrasensitive detections, thus, providing a wealth of information regarding the biological mechanisms in living systems. Despite fluorescence analysis has advanced remarkably, conventional detections still encounter considerable limitations.

Decreased infectivity of a neutralization-resistant equine infectious anemia virus variant can be overcome by efficient cell-to-cell spread.

Two variants of equine infectious anemia virus (EIAV) that differed in sensitivity to broadly neutralizing antibody were tested in direct competition assays. No differences were observed in the growth curves and relative fitness scores of EIAVs of principal neutralizing domain variants of groups 1 (EIAV(PND-1)) and 5 (EIAV(PND-5)), respectively; however, the neutralization-resistant EIAV(PND-5) variant was less infectious in single-round replication assays. Infectious center assays indicated similar rates of cell-to-cell spread, which was approximately 1,000-fold more efficient than cell-free infectivity.

Protective effects of broadly neutralizing immunoglobulin against homologous and heterologous equine infectious anemia virus infection in horses with severe combined immunodeficiency.

Using the equine infectious anemia virus (EIAV) lentivirus model system, we previously demonstrated protective effects of broadly neutralizing immune plasma in young horses (foals) with severe combined immunodeficiency (SCID). However, in vivo selection of a neutralization-resistant envelope variant occurred. Here, we determined the protective effects of purified immunoglobulin with more potent broadly neutralizing activity.

The nuclear architectural protein HMGA1a triggers receptor-mediated endocytosis.

High mobility group proteins A (HMGA), nuclear architectural factors, locate in the cell nuclei and mostly execute gene-regulation function. However, our results reveal that a HMGA member (HMGA1a) has a unique plasma membrane receptor; this receptor specifically binds to HMGA-decorated species, effectively mediates endocytosis, and internalizes extracellular HMGA-functionalized cargoes. Indeed, dyes or nanoparticles labeled with HMGA1a protein readily enter Hela cells.

Single-chromophore-based photoswitchable nanoparticles enable dual-alternating-color fluorescence for unambiguous live cell imaging.

We have developed a class of spiropyran dyes and their fluorescence colors can be reversibly photoswitched from red to green, blue, or nearly dark, thus alternating between two colors. Such individual dyes emit either one color or the other but not both simultaneously. Nanoparticles enabled with these photoswitchable dyes, however, emit either one pure color or a combination of both colors because the nanoparticle fluorescence originates from multiple dyes therein.