Viability of in traditional sour pork fermentation and its inactivation by microwave heating: Implications for zoonotic risk and food safety.

Background And Aim: Cultural dietary practices involving the consumption of raw or undercooked meat, such as traditional sour fermented pork, pose significant risks for foodborne parasitic infections, particularly trichinellosis caused by . This study aimed to evaluate the viability of larvae during sour pork fermentation and to assess the efficacy of microwave heating as a practical method for inactivating the larvae.

Materials And Methods: Laboratory-bred hamsters were experimentally infected with to obtain encysted muscle larvae.

Epidemiological Surveillance of Intestinal Parasites and Serological Analysis of in Captive Felids From Thailand Zoos.

Wild felids have been in decline, which has been linked to health issues. Parasitic infections, including zoonotic ones, can contribute to the challenges faced regarding wildlife conservation efforts and may pose a risk to human health, particularly for people working at or visiting zoological parks. This study investigated the occurrence of parasitic infections in the Felidae within Thai zoo parks.

Development of ready-to-use microtiter plates for enhanced ELISA detection of Opisthorchis viverrini antigens in urine samples.

A monoclonal antibody against Opisthorchis viverrini antigen (OvAg) immobilization step for OvAg detection in urine samples by ELISA significantly influences the reduction in overall procedural time to conduct the assay. Herein, the monoclonal antibody against OvAg immobilization step was improved using a combining approach between a crosslinking agent, APTES, and a coupling agent EDC-NHS. Results showed that the combining approach method is effective when immobilization of monoclonal antibody is applied for approximately 30 min providing excellent potential for OvAg detection by ELISA.

Tricky with Heat and Salt: Soil Factors, Thermotaxis, and Potential for Heat-Saline Agar Trapping of Larvae.

The viability and host-seeking behavior of larvae are significantly influenced by soil conditions, emphasizing the critical role of environmental control in disease management. This is particularly relevant given the growing concerns about drug resistance resulting from mass chemotherapy or the use of chemical nematicides. was effectively inactivated by exposure to 50 °C for both 12 and 24 h (long-term exposure).

Mapping malaria in Thailand: A Bayesian spatio-temporal analysis of national surveillance data.

Objectives: Malaria, caused by protozoan parasites of the genus Plasmodium, remains prevalent in tropical and subtropical regions. This study employed Bayesian spatio-temporal analysis to assess malaria incidence patterns and identify environmental and climatic correlates across Thailand at the district level.

Methods: We analysed national malaria surveillance data using Bayesian hierarchical models to examine spatio-temporal patterns in malaria incidence.

Investigation of malaria vectors Anopheles in non-endemic areas of Thailand: in proximity to workplaces housing foreign migrant workers.

Background: Foreign migrant workers from malaria-endemic regions play a critical factor in the transmission of malaria to non-endemic areas, mainly due to their mobility while seeking employment opportunities. This risk is particularly heightened in areas where malaria vectors are present.

Methods: This study aimed to investigate the malaria vectors in two sub-districts in Khon Kaen Province, known for their factory areas and the significant presence of Myanmar migrant worker communities.

Genomic analysis and virulence of human Streptococcus suis serotype 14.

Purpose: Streptococcus suis serotype 14 is the second most prevalent serotype being highly prevalent in Southeast Asia. This study aimed to characterize genetic background, population structure, virulent genes, antimicrobial-resistant genes, and virulence of human S. suis serotype 14.

Spatial prediction of the probability of liver fluke infection in water resource within sub-basin using an optimized geographically-weighted regression model.

Introduction: Infection with liver flukes (Opisthorchis viverrini) is partly attributed to their ability to thrive in sub-basin habitats, causing the intermediate host to remain within the watershed system throughout the year. It is crucial to conduct spatial monitoring of fluke infection at a small basin analysis scale as it helps in studying the spatial factors influencing these infections. The number of infected individuals was obtained from local authorities, converted into a percentage, and visually represented as raster data through a heat map.

EVALUATION OF THE CYSTICIDAL ACTIVITY OF CHLOROCRESOL AGAINST ACANTHAMOEBA POLYPHAGA.

Chlorocresol has antibacterial and antifungal properties, yet its effectiveness in eradicating Acanthamoeba spp. remains unexplored. Acanthamoeba species trophozoites are usually sensitive to biocides, whereas cysts tend to be more resistant.

Gastrointestinal Helminthic Infection among the Population in Northern Thailand.

Purpose: Gastrointestinal (GI) helminthic infections pose substantial public health threat, particularly in northern Thailand, with a heightened concern in Nan province. This study aims to evaluate the prevalence of GI helminthic infections in Nan province and identify associated risk factors in local population.

Methods: A cross-sectional survey was conducted in 2022.

Misclassification of Opisthorchis viverrini and Minute Intestinal Fluke Eggs by Routine Laboratory Staff Using Images from the Kato-Katz Method.

Background: The Kato-Katz method is a commonly used diagnostic tool for helminth infections, particularly in field studies. This method can yield inaccurate results when samples contain eggs that are similar in appearance, such as Minute Intestinal Fluke (MIF) and Opisthorchis viverrini (OV) eggs. The close resemblance of eggs can be problematic and raises the possibility of false diagnoses.

Formalin-fixed stool improves the performance of the Kato-Katz method.

Background And Aim: Parasitic infections are a public health problem worldwide, including in Thailand. An epidemiological survey for helminthiasis based on stool examination uses the Kato-Katz method as recommended by the World Health Organization. Limitations of this method include the need for fresh stool, time requirement, and lack of quality control.

Prevalence of gastrointestinal parasites in captive mammals at Khon Kaen Zoo, Thailand.

Background And Aim: Captive animals are susceptible to parasitic diseases due to the stress and confinement they experience. In addition, they can serve as reservoirs of zoonotic parasites that have the potential to infect humans. To investigate this possibility, we estimated the prevalence of gastrointestinal (GI) parasites in captive mammals at Khon Kaen Zoo, Thailand.

Comparison of 3 DNA extraction methods for extracting DNA from an adult Culex quinquefasciatus (Diptera: Culicidae).

The aim of this study was to compare 3 DNA extraction methods: the PureLink Genomic DNA kit, DNAzol Direct reagent, and a microwave-based method, for extracting DNA from an adult Culex quinquefasciatus by focusing on the quantity and purity of DNA, cost, and time required. Ten mosquitoes were individually used for DNA extraction by each method. Based on the results obtained, DNA was extracted from each method using specific primers, resulting in a polymerase chain reaction (PCR) product with a length of 274 bp.

Reduction of carcinogens in fermented fish (pla-ra and pla-som) by heating.

Background And Aim: The risk factors for cholangiocarcinoma (CCA) are opisthorchiasis and the intake of a combination of nitroso compounds through the consumption of traditionally fermented fish, which is very popular in areas where liver flukes are endemic. The incidence of CCA remains high because this cultural habit of rural people has been altered. Therefore, decreasing nitrate and nitrite concentrations in fermented fish are an alternative approach to reducing the risk of CCA.

Endoplasmic Reticulum Stress-Mediated Apoptosis Induced by VR12684 Isolated from Mallotus spodocarpus in Cholangiocarcinoma Cell Line.

Introduction: Cholangiocarcinoma (CCA) is a poor prognosis of a malignant tumor that has been unresponsive to conventional chemotherapeutic agents. Effective and novel therapeutic agents are urgently needed. VR12684 (isolated from Mallotus spodocarpus) has been reported to exhibit growth inhibitory activities in cancer cell lines.

Correction: Perakanya et al. Prevalence and Risk Factors of Infection in Sakon Nakhon Province, Thailand. 2022, , 313.

The authors wish to make the following corrections to this paper [...

Monks: A Population at Risk for Liver Fluke and Skin-Penetrating Helminths.

Monks cannot cook received raw meat dishes and should walk barefoot while working. This population lacks both a survey of parasitic infection and a proper prevention and control policy. Five hundred and fourteen monks from the Ubolratana, Ban Haet, and Ban Phai Districts of Kh on Kaen Province were enrolled in this study.

Prevalence and Associated Risk Factors of Intestinal Parasitic Infections: A Population-Based Study in Phra Lap Sub-District, Mueang Khon Kaen District, Khon Kaen Province, Northeastern Thailand.

Intestinal parasitic infections are still a crucial problem among communities in Northeast Thailand. Misuse of antiparasitic drugs and unhealthy food behaviors are known. This study aimed to explore the prevalence, behavioral health factors, and motivation for self-treatment of anti-parasitic drugs in this area.

Prevalence and Risk Factors of Infection in Sakon Nakhon Province, Thailand.

Opisthorchiasis is a parasitic infection caused by the liver fluke . This parasite is widely distributed and well documented in Thailand, Lao PDR, Southern Vietnam, Cambodia, and Myanmar. However, its prevalence is a major problem in these countries.

Green-synthesized silver nanoparticle coating on paper for antibacterial and antiviral applications.

The use of active packaging has attracted considerable attention over recent years to prevent and decrease the risk of bacterial and viral infection. Thus, this work aims to develop active packaging using a paper coated with green-synthesized silver nanoparticles (AgNPs). Effects of different silver nitrate (AgNO) concentrations, viz.

Myiasis in Ulcerated Breast Carcinoma: First Case Record in Myanmar.

Myiasis is an infestation of maggot, which is frequently associated with poor personal hygiene and environmental sanitation. A 78-year-old female breast cancer patient visited clinic complaining of irritation, itching, and pain within the ulcerous cancer lesion for 3 weeks. Many maggots were found in the lesion.