Power spectrum curve compensation method for image quality enhancement in high-throughput gene sequencing.

In high-throughput gene sequencing, the quality of sequencing images is critical for the accuracy of subsequent base calling. However, during practical sequencing processes, the time delay integration (TDI) camera's push-scan imaging often leads to significant degradation of image quality along the push-scan direction. Addressing the current limitations in TDI image restoration research for gene sequencing, this study establishes an imaging spectrum model of sequencing images based on MGI's ultra-high-throughput sequencer.