Mechanical Failures as Predicted by Achieving Local vs Global T4-L1 Hip Axis Goals: A Single Center Experience.

Study Design: Retrospective cohort study.

Objective: To evaluate the predictive value of T4-L1 Hip Axis Error (HAE) and L1PA Error (L1PAE) on mechanical failure following adult spinal deformity (ASD) surgery, and to assess how error directionality and fusion length influence outcomes.

Summary Of Background Data: The T4-L1 Hip Axis is a novel framework for defining normative sagittal alignment by aligning thoracic and lumbar curvatures relative to the pelvis.

Accuracy of the Automated Range of Motion Observer and Reporter Software for Fully Automated Joint Measurement From Patient Videos.

Introduction: Accurate assessment of joint range of motion (ROM) is essential for diagnosing and managing upper extremity injuries. Universal goniometers are the most used tools for measuring ROM, but they require skilled observers and are limited by interobserver variability. An automated system for measuring joint range of motion from patient videos could facilitate standardized reporting of outcomes after reconstructive surgery.

Validation and clinical application of the ΔC2 pelvic angle - ΔC2 tilt = Δpelvic tilt equation for predicting pelvic tilt in spinal deformity surgery.

Objective: Notably, studies have established a consistent link between global sagittal alignment and pelvic tilt (PT) using C2 tilt (C2T) and C2 pelvic angle (C2PA), described by the following equation: C2PA = PT + C2T. The present study aimed to validate the proposed relationship (predicted ΔPT = ΔC2PA - ΔC2T) based on the assumption that patients aim to maintain a neutral C2T. Additionally, this study sought to evaluate the accuracy of intraoperative C2PA measurements for predicting postoperative PT.

Analysis of CDPK1 targets identifies a trafficking adaptor complex that regulates microneme exocytosis in .

Apicomplexan parasites use Ca-regulated exocytosis to secrete essential virulence factors from specialized organelles called micronemes. Ca-dependent protein kinases (CDPKs) are required for microneme exocytosis; however, the molecular events that regulate trafficking and fusion of micronemes with the plasma membrane remain unresolved. Here, we combine sub-minute resolution phosphoproteomics and bio-orthogonal labeling of kinase substrates in to identify 163 proteins phosphorylated in a CDPK1-dependent manner.

A positive feedback loop controls Toxoplasma chronic differentiation.

Successful infection strategies must balance pathogen amplification and persistence. In the obligate intracellular parasite Toxoplasma gondii this is accomplished through differentiation into dedicated cyst-forming chronic stages that avoid clearance by the host immune system. The transcription factor BFD1 is both necessary and sufficient for stage conversion; however, its regulation is not understood.

Analysis of CDPK1 targets identifies a trafficking adaptor complex that regulates microneme exocytosis in .

Apicomplexan parasites use Ca-regulated exocytosis to secrete essential virulence factors from specialized organelles called micronemes. Ca-dependent protein kinases (CDPKs) are required for microneme exocytosis; however, the molecular events that regulate trafficking and fusion of micronemes with the plasma membrane remain unresolved. Here, we combine sub-minute resolution phosphoproteomics and bio-orthogonal labeling of kinase substrates in to identify 163 proteins phosphorylated in a CDPK1-dependent manner.