Enhancement of the triplet photosensitizing ability of BODIPY dyes by controlled red-shifted H-aggregate formation using ionic liquids: insights from spectroscopy, microscopy, DFT and biological studies.

We report an innovative, straightforward, and effective supramolecular method for converting fluorophores into photosensitizers using symmetrical imidazolium ionic liquids (ILs) the aggregation of BODIPY derivatives. By utilizing "red-shifted H-aggregates" of 2,6-diethyl-4,4-difluoro-1,3,5,7-tetramethyl-8-phenyl-4-bora-3a,4a-diaza--indacene (R6-BDP), along with both shorter and longer cationic alkyl chain symmetrical ILs, we enhanced the conversion efficiency of the R6-BDP aggregates from fluorophores to photosensitizers. The optical properties of R6-BDP in both the molecular and aggregated states were investigated through UV-visible, fluorescence and time-resolved fluorescence spectroscopy techniques.

Radical cascade cyclization of amino acid-tethered 1,6-enynones with sulfonyl hydrazides for N-terminal modification: synthesis of functionalized succinimide derivatives.

A metal-free strategy for the N-terminal cyclization of amino acids has been developed by synthesizing highly functionalized succinimide derivatives through radical cyclization of amino acid-tethered 1,6-enynones with sulfonyl hydrazide using NIS and HO as an oxidant. The notable advantages of this work includes time-efficient, good / ratio, moderate to good yields, and was synthesized on a gram-scale. Furthermore, the synthetic utility of the product 5aa was performed by (i) Suzuki coupling reaction with iodo-functionality; and (ii) dipeptide formation using glycine methyl ester.

An isophorone-derived AIE-active probe for peroxynitrite detection and bioimaging applications.

Compounds exhibiting aggregation-induced emission (AIE) are remarkable due to their characteristic restriction of intramolecular rotation, making them highly versatile for a wide range of applications. Herein, an isophorone based probe (IHP) was developed for the detection of peroxynitrite (ONOO) using the C[double bond, length as m-dash]N as the reactive group. Photophysical investigations were conducted to explore the aggregation behaviour of IHP in two different solvent systems: DMSO:PBS and DMSO.

New spirooxindole pyrrolidine/pyrrolizidine analogs: design, synthesis, and evaluation as an anticancer agent.

A novel series of mesitylene-based spirooxindoles were synthesized the multicomponent [3 + 2] cycloaddition reaction in a greener medium. Spectroscopic techniques such as H and C NMR and HRMS analysis were carried out for the structural elucidation of all the spirooxindole derivatives. The cytotoxicity properties of spirooxindole analogs 4/5(a-g) against the human lung (A549) cancer cell line exhibited encouraging outcomes.

A near-infrared julolidine probe for visualization of mitochondrial peroxynitrite in living cells.

The overproduction of peroxynitrite (ONOO) in mitochondria has been associated with various pathophysiological conditions and disorders. However, the use of fluorescent probes to visualize mitochondrial ONOO in biological systems is limited due to their low emission wavelengths and small Stokes shifts, which present significant challenges. In this study, we designed and synthesized julolidine-based near-infrared (NIR) fluorescent probes, named JQMe and JCN, specifically to monitor mitochondrial ONOO.

Comparative Study of Fluorophores for Precise Dopamine Detection and Investigation of Its Association with Stress and Coffee Addiction in HEK 293 Cells.

In today's world, where stress and addiction are increasingly prevalent due to job pressures and coping mechanisms, dopamine (Dopa), a key hormone linked to mood, happiness, and mental health, has become vital for understanding conditions like depression and anxiety. Our study focuses on detecting Dopa pathways both in vitro using HEK293 cells and in vivo using zebrafish under stress and addiction conditions. We employed a biocompatible organic fluorophore (P1), with pyrazole-4-carboxaldehyde as the recognition unit, which demonstrated a detection limit of 8.

Development of a xanthene-based NIR fluorescent probe for accurate and sensitive detection of γ-glutamyl transpeptidase in cancer diagnosis and treatment.

γ-Glutamyl transpeptidase (GGT) regulates glutathione (GSH), essential for cell functions and linked to cancer. High GGT levels in tumors make it a valuable cancer biomarker. Current GGT detection methods often lack sensitivity and specificity.

Natural product-inspired [3 + 2] cycloaddition-based spirooxindoles as dual anticancer agents: synthesis, characterization, and biological evaluation by and methods.

Breast and colorectal cancers are the most common tumors, with high recurrence and low survival rates. We designed and synthesized a series of spirooxindole pyrrolidinyl derivatives, which were further evaluated for anti-proliferative activity using MDA-MB-468 and HCT 15 cell lines. The best inhibitor of this class, compound 6f, showed a very good inhibition potency, both on the MDA-MB-468 and HCT 15 cells as confirmed by molecular docking and molecular dynamic studies that predicted its binding mode into the active site of the targets.

Environmentally Sustainable Detection of Arsenic using Convolutional Neural Networks and Imidazole-Based Organic Probes: Application in Food Samples and Arsenic Album.

Arsenic contamination poses a significant health risk, particularly when it infiltrates water supplies. While current detection methods offer precise analysis, they often involve complex instrumentation not suitable for field use. This study presents a novel approach by developing two probes, A1 and A2, based on 4-diethylaminosalicyladehyde, 2-hydroxy-1-naphthaldehyde, and 1,2-diaminoanthraquinone.

A near-infrared fluorescent probe with a substantial Stokes shift designed for the detection and imaging of β-galactosidase within living cells and animals.

β-Galactosidase serves as a pivotal biomarker for both cancer and cellular aging. The advancement of fluorescent sensors for tracking β-galactosidase activity is imperative in the realm of cancer diagnosis. We have designed a near-infrared fluorescent probe (PTA-gal) for the detection of β-galactosidase in living systems with large Stokes shifts.

A fluorescence turn "on-off" imaging probe for sequential detection of Al and L-Cysteine in HeLa cells.

At this "Aluminum Age", exposure to aluminum (metallic or ionic form) is inevitable and inestimable. The presence of aluminum in biological systems is evident but more often aluminum toxicity is less understood. Therefore, the presence of biologically reactive aluminum needs to be identified and quantified.

Ratiometric Fluorescent Probe for the Detection of Nanomolar Phosgene in Solution and Gaseous Phase: Advancing Crime Detection Applications.

Phosgene, an exceptionally hazardous gas, poses a grave concern for the health and safety of the general public. The present study describes a fluorescent ratiometric probe for phosgene employing 2-(naphthalen-2-yl) benzo[d]oxazol-5-amine (NOA) with an amino group as the recognition site. NOA detects phosgene through the intramolecular charge transfer mechanism.

Single Step Solid State Synthesis of Carbon Nanoparticles for Instantaneous Detection of Fe (III) in Water Samples.

Though iron is one of the vital micronutrients in biological systems excess of which is associated with various illness. Consumption of contaminated water and crops because of its extensive industrial utility is one of the major sources for excess iron in living beings. Hence, we have designed a sensor based on carbon nanoparticles for the detection of Fe (III) and we have also attempted to estimate Fe (III) in spiked water samples.

A Study of Small Molecule-Based Rhodamine-Derived Chemosensors and their Implications in Environmental and Biological Systems from 2012 to 2021: Latest Advancement and Future Prospects.

Rhodamine-based chemosensors have sparked considerable interest in recent years due to their remarkable photophysical properties, which include high absorption coefficients, exceptional quantum yields, improved photostability, and significant red shifts. This article presents an overview of the diverse fluorometric, and colorimetric sensors produced from rhodamine, as well as their applications in a wide range of fields. The ability of rhodamine-based chemosensors to detect a wide range of metal ions, including Hg, Al, Cr, Cu, Fe, Fe, Cd, Sn, Zn, and Pb, is one of their major advantages.

Expeditious fluorimetric detection of bilirubin by simple imidazole derived luminophore and it's pragmatic applicability in spiked biological fluids.

Bilirubin is an indispensable biomarker for liver diseases. Utilizing organic molecules as sensor platform for effective detection of bilirubin are little. In addition, the reported fluorophores required longer incubation time for detection.

Dual-Responsive Benzo-Hemicyanine-Based Fluorescent Probe for Detection of Cyanide and Hydrogen Sulfide: Real-Time Application in Identification of Food Spoilage.

Colorimetric and fluorescent probes have received a lot of attention for detecting lethal analytes in realistic systems and in living things. Herein, a dual-approachable Benzo-hemicyaninebased red-emitting fluorescent probe PBiSMe, for distinct and instantaneous detection of CN and HS was synthesized. The PBiSMe emitted red fluorescence (570 nm) can switch to turn-off (570 nm) and blue fluorescence (465 nm) in response to CN and HS, respectively.

Benzodioxole grafted spirooxindole pyrrolidinyl derivatives: synthesis, characterization, molecular docking and anti-diabetic activity.

A highly stereoselective, three-component method has been developed to synthesize pyrrolidine and pyrrolizidine containing spirooxindole derivatives. The interaction between the dipolarophile α,β-unsaturated carbonyl compounds and the dipole azomethine ylide formed by the reaction of 1,2-dicarbonyl compounds and secondary amino acids is referred to as the 1,3-dipolar cycloaddition reaction. The reaction conditions were optimized to achieve excellent stereo- and regioselectivity.

Recent Advancements in the Design and Development of Near Infrared (NIR) Emitting Fluorescent Probes for Sensing and their Bio-Imaging Applications.

Fluorescent bio-imaging will be the future in the medical diagnostic for visualising inner cellular and tissues. Near-infrared (NIR) emitting fluorescent probes serve dynamically for targeted fluorescent imaging of live cells and tissues. NIR imaging is advantageous because of its merits like deep tissue penetration, minimum damage to the tissue, reduced auto fluorescence from the background, and improved resolution in imaging.

A two photon fluorescent probe for highly selective detection and endogenous imaging of hydrogen sulfide.

Hydrogen sulfide (HS), one of redox-active sulfur species, is known as a signaling molecule and an antioxidant in biological tissues to maintain cellular functions. The development of selective and sensitive HS detection is important to understand the role of HS in vivo. Herein, a new two-photon probe NNE was developed to detect hydrogen sulfide using 6-acetyl-N-methyl-2-naphthylamine with an attachment of 7-nitrobenzo-oxadiazole.

"Covalent-Assembly"-Triggered Striking Far-Red to near-Infrared Emitting Fluorescent Probe for Abrupt Detection of Nerve-Agent Mimic (DCP): Real Time Application in Monitoring the Presence of Trace Amounts in Soil and Live Cells.

Detection of chemical warfare agents (CWA) by simple and rapid methods with real-sample applications are quite inevitable in order to ease the threats to living systems caused by uncertain terror attacks and wars. Herein we have developed the first far-red to near infra-red (NIR) probe based on a covalent assembly approach for the detection of trace amounts of nerve agent mimic diethyl chloro phosphate (DCP) in soil and their fluorescent bio imaging in live cells. The probe features abrupt fluorescence turn on sensing of DCP with fluorescence quantum yield Φ = 0.

Specific two-photon fluorescent probe for cysteine detection in vivo.

Cysteine (Cys), an essential amino acid, plays several crucial functions in numerous biological processes. Notably, the detection of Cys is critical to disease diagnosis. Fluorescent probes that can quickly detect Cys will help to study the mechanism of certain diseases.