Crystal Habits as Potential Biosignatures.

Our understanding of crystalline structures within terrestrial planetary analog environments can shed light on how these features can be interpreted on rocky planets and icy moons in our solar system. The ability to distinguish biogenic and abiotic components within the mineral, crystal, and structural features allows us to inform future life detection missions, science payloads, and instrument measurement resolutions. Moreover, having these terrestrial reference measurements in a review format allows the measurement rationale to be understood in the context of mission concepts and geomicrobiological assessment of life in extreme environments.

Laminae as Potential Biosignatures.

Laminae are millimeter-scale features in rocks created by physiochemical processes that can be influenced by the presence and activities of communities of organisms that occur as biofilms and microbial mats. The structure and composition of laminae reflect the processes involved in their formation and can be preserved in the rock record over geologic time; however, diagenetic and metamorphic alteration can lead to the loss of primary information and confusion over the interpretation of their origins. As potential records of ancient life, laminae can preserve evidence of microbial activity over billions of years of Earth's history.

Life Detection Knowledge Base: Taxonomy of Potential Biosignatures.

The Life Detection Knowledge Base (LDKB) is a community webtool developed to test and evaluate strategies to search for evidence of life beyond Earth, with an emphasis on recognizing potential false-positive and false-negative results. As part of the LDKB framework, we developed a taxonomy of potential biosignatures. The taxonomy brings together a broad array of life-detection strategies into a common and systematic structure that allows for equitable evaluations based on a specific set of criteria, chosen to assess the likelihood of false-positive and false-negative interpretations.

Structural Biosignatures-A Category of Potential Biosignatures in the Life Detection Knowledge Base.

The Life Detection Knowledge Base (LDKB) is part of the Life Detection Forum suite of web tools developed for life detection mission planners. This article details the development of one of its categories of biosignatures, the category. The category includes physical attributes of objects and their spatial relationships (e.

Metabolic versatility of from geothermal features of Hawai'i and Chile as revealed by five metagenome-assembled genomes.

Members of the archaeal order (previously the phylum Aigarchaeota) are poorly sampled and are represented in public databases by relatively few genomes. Additional representative genomes will help resolve their placement among all known members of and provide insights into their roles in the environment. In this study, we analyzed 16S rRNA gene amplicons belonging to the that are available in public databases, which demonstrated that archaea of the order are diverse, widespread, and most abundant in geothermal habitats.

Assessing siliceous sinter matrices for long-term preservation of lipid biomarkers in opaline sinter deposits analogous to Mars in El Tatio (Chile).

Subaerial hydrothermal systems are of great interest for paleobiology and astrobiology as plausible candidate environments to support the origin of life on Earth that offer a unique and interrelated atmosphere-hydrosphere-lithosphere interface. They harbor extensive sinter deposits of high preservation potential that are promising targets in the search for traces of possible extraterrestrial life on Hesperian Mars. However, long-term quality preservation is paramount for recognizing biosignatures in old samples and there are still significant gaps in our understanding of the impact and extent of taphonomy processes on life fingerprints.

Cellular remains in a ~3.42-billion-year-old subseafloor hydrothermal environment.

Subsurface habitats on Earth host an extensive extant biosphere and likely provided one of Earth's earliest microbial habitats. Although the site of life's emergence continues to be debated, evidence of early life provides insights into its early evolution and metabolic affinity. Here, we present the discovery of exceptionally well-preserved, ~3.

ExoMars Mars Organic Molecule Analyzer (MOMA) Laser Desorption/Ionization Mass Spectrometry (LDI-MS) Analysis of Phototrophic Communities from a Silica-Depositing Hot Spring in Yellowstone National Park, USA.

The Mars Organic Molecule Analyzer (MOMA) is a key scientific instrument on the ExoMars Rover mission. MOMA is designed to detect and characterize organic compounds, over a wide range of volatility and molecular weight, in samples obtained from up to 2 m below the martian surface. Thorough analog sample studies are required to best prepare to interpret MOMA data collected on Mars.

Microbial Biomarker Transition in High-Altitude Sinter Mounds From (Chile) Through Different Stages of Hydrothermal Activity.

Geothermal springs support microbial communities at elevated temperatures in an ecosystem with high preservation potential that makes them interesting analogs for early evolution of the biogeosphere. The geysers field in the Atacama Desert has astrobiological relevance due to the unique occurrence of geothermal features with steep hydrothermal gradients in an otherwise high altitude, hyper-arid environment. We present here results of our multidisciplinary field and molecular study of biogeochemical evidence for habitability and preservation in silica sinter at .

Planetary Protection and Mars Special Regions--A Suggestion for Updating the Definition.

We highlight the role of COSPAR and the scientific community in defining and updating the framework of planetary protection. Specifically, we focus on Mars "Special Regions," areas where strict planetary protection measures have to be applied before a spacecraft can explore them, given the existence of environmental conditions that may be conducive to terrestrial microbial growth. We outline the history of the concept of Special Regions and inform on recent developments regarding the COSPAR policy, namely, the MEPAG SR-SAG2 review and the Academies and ESF joint committee report on Mars Special Regions.

The United States Culture Collection Network (USCCN): Enhancing Microbial Genomics Research through Living Microbe Culture Collections.

The mission of the United States Culture Collection Network (USCCN; http://usccn.org) is "to facilitate the safe and responsible utilization of microbial resources for research, education, industry, medicine, and agriculture for the betterment of human kind." Microbial culture collections are a key component of life science research, biotechnology, and emerging global biobased economies.

Production and early preservation of lipid biomarkers in iron hot springs.

The bicarbonate-buffered anoxic vent waters at Chocolate Pots hot springs in Yellowstone National Park are 51-54°C, pH 5.5-6.0, and are very high in dissolved Fe(II) at 5.

Reversible inactivation and desiccation tolerance of silicified viruses.

Long-distance host-independent virus dispersal is poorly understood, especially for viruses found in isolated ecosystems. To demonstrate a possible dispersal mechanism, we show that bacteriophage T4, archaeal virus Sulfolobus spindle-shaped virus Kamchatka, and vaccinia virus are reversibly inactivated by mineralization in silica under conditions similar to volcanic hot springs. In contrast, bacteriophage PRD1 is not silicified.

Potential fossil endoliths in vesicular pillow basalt, Coral Patch Seamount, eastern North Atlantic Ocean.

The chilled rinds of pillow basalt from the Ampère-Coral Patch Seamounts in the eastern North Atlantic were studied as a potential habitat of microbial life. A variety of putative biogenic structures, which include filamentous and spherical microfossil-like structures, were detected in K-phillipsite-filled amygdules within the chilled rinds. The filamentous structures (∼2.

Morphological biosignatures and the search for life on Mars.

This report provides a rationale for the advances in instrumentation and understanding needed to assess claims of ancient and extraterrestrial life made on the basis of morphological biosignatures. Morphological biosignatures consist of bona fide microbial fossils as well as microbially influenced sedimentary structures. To be recognized as evidence of life, microbial fossils must contain chemical and structural attributes uniquely indicative of microbial cells or cellular or extracellular processes.

Microbial composition of near-boiling silica-depositing thermal springs throughout Yellowstone National Park.

The extent of hyperthermophilic microbial diversity associated with siliceous sinter (geyserite) was characterized in seven near-boiling silica-depositing springs throughout Yellowstone National Park using environmental PCR amplification of small-subunit rRNA genes (SSU rDNA), large-subunit rDNA, and the internal transcribed spacer (ITS). We found that Thermocrinis ruber, a member of the order Aquificales, is ubiquitous, an indication that primary production in these springs is driven by hydrogen oxidation. Several other lineages with no known close relatives were identified that branch among the hyperthermophilic bacteria.