Portable lensfree imaging platform based on prior-guided phase retrieval.

We presented a portable lensfree imaging platform that can achieve complex wavefield reconstruction with multi-distance intensity measurements. In our platform, 400 LEGO bricks and a bare CMOS sensor chip are integrated into a lensfree in-line holographic imaging system, where a motor and a set of gear modules from LEGO are designed to control the axial movement of a sample to generate multi-plane intensity patterns. In data processing, the intensity images are calculated in a computer to show the retrieved amplitude and phase of the sample.

Single-shot lensless masked imaging with enhanced self-calibrated phase retrieval.

Single-shot lensless imaging with a binary amplitude mask enables a low-cost and miniaturized configuration for wave field recovery. However, the mask only allows a part of the wave field to be captured, and thus the inverse decoding process becomes a highly ill-posed problem. Here we propose an enhanced self-calibrated phase retrieval (eSCPR) method to realize single-shot joint recovery of mask distribution and the sample's wavefront.

Dual-constrained physics-enhanced untrained neural network for lensless imaging.

An untrained neural network (UNN) paves a new way to realize lensless imaging from single-frame intensity data. Based on the physics engine, such methods utilize the smoothness property of a convolutional kernel and provide an iterative self-supervised learning framework to release the needs of an end-to-end training scheme with a large dataset. However, the intrinsic overfitting problem of UNN is a challenging issue for stable and robust reconstruction.

Lensless masked imaging with self-calibrated phase retrieval.

Lensless imaging with a mask is an attractive topic as it enables a compact configuration to acquire wavefront information of a sample with computational approaches. Most existing methods choose a customized phase mask for wavefront modulation and then decode the sample's wave field from modulated diffraction patterns. Different from phase masks, lensless imaging with a binary amplitude mask facilitates a cheaper fabrication cost, but high-quality mask calibration and image reconstruction have not been well resolved.

Lensfree on-chip microscopy based on single-plane phase retrieval.

We propose a novel single-plane phase retrieval method to realize high-quality sample reconstruction for lensfree on-chip microscopy. In our method, complex wavefield reconstruction is modeled as a quadratic minimization problem, where total variation and joint denoising regularization are designed to keep a balance of artifact removal and resolution enhancement. In experiment, we built a 3D-printed field-portable platform to validate the imaging performance of our method, where resolution chart, dynamic target, transparent cell, polystyrene beads, and stained tissue sections are employed for the imaging test.

Image scanning microscopy with a long depth of focus generated by an annular radially polarized beam.

Image scanning microscopy (ISM) is a promising tool for bioimaging owing to its integration of signal to noise ratio (SNR) and super resolution superior to that obtained in confocal scanning microscopy. In this paper, we introduce the annular radially polarized beam to the ISM, which yields an axially extended excitation focus and enhanced resolution, providing a new possibility to obtain the whole information of thick specimen with a single scan. We present the basic principle and a rigorous theoretical model for ISM with annular radially polarized beam (ISM-aRP).