Four-year experience with more than 1000 cases of total laparoscopic liver resection in a single center.

Background: Laparoscopic liver resection (LLR) has become a safe surgical procedure that needs additional summarization.

Aim: To review 4 years of total LLR surgeries, exceeding 1000 cases, which were performed at a single center.

Methods: Patients who underwent LLR at West China Hospital of Sichuan University between January 2015 and December 2018 were identified.

Dynamic DNA Shortening by Telomere-Binding Protein Cdc13.

Telomeres are essential for chromosome maintenance. Cdc13 is a single-stranded telomeric DNA binding protein that caps telomeres and regulates telomerase function in yeast. Although specific binding of Cdc13 to telomeric DNA is critical for telomere protection, the detail mechanism how Cdc13-DNA complex protects telomere is unclear.

[Mechanism of Inorganic Nitrogen Transformation and Identification of Nitrogen Sources in Water and Soil].

Nitrogen plays a vital role in biological activities as the basic element of organic molecules and the main nutrient of soil. In the study of nitrogen pollution, the first step is to understand the transformation mechanism of various nitrogen forms. Based on the transformation process and the mechanism of nitrogen in the ecosystem, this review summarizes the research methods of nitrogen source analysis and nitrogen isotope fractionation in soil and water, and categorically reviews the applications of nitrogen source identification in surface water and groundwater.

[Effects and mechanism of hypoxia exposure on related genes in brown fat tissue of obese mice based on mRNA expression profile microarray].

Objective: This study intended to screen differentially expressed genes and pathways in Brown Adipose Tissue (BAT) of obese mice after the intervention of hypoxia by mRNA expression profile microarray, exploring the mechanism of hypoxia activated BAT.

Methods: Thirty C57BL/6J male mice were divided into the normal diet control group (N, =8), high-fat diet control group (OB, =8) and high-fat diet hypoxia group (H, =8). Group H was intervened by hypoxia exposure in the oxygen concentration of 11.