Comparison of Cytotoxicity and Photocatalytic Properties of Iron Vanadate Nanoparticles with Commercial Catalysts: For the Degradation of Microplastics and Bacterial Inactivation Application.

Microplastics (MPs) and the development of associated antibiotic-resistant bacteria are of serious concern. Conventional water treatment methodologies do not sufficiently address the issue of MPs and MPs-attached bacteria. The photocatalytic process is a promising technique that utilizes solar light to generate HO radicals for the degradation of MPs and inactivation of microorganisms.

New Quipazine Derivatives Active Against Drug-Resistant Oncogenic Strains with Biofilm.

() is regarded as a significant risk factor for gastritis, peptic ulcer disease, and gastric cancer. However, the increasing resistance of strains has resulted in low eradication rates and ineffective treatments. Herein, we report on identification of a new quipazine derivative-compound (-(3-chlorobenzyl)-2-(piperazin-1-yl)quinolin-4-amine), which displayed antibacterial properties (MIC range 2-4 µg/mL) against CagA-positive reference strains associated with an increased risk of gastric cancer, including metronidazole-resistant ATCC 43504, clarithromycin-resistant ATCC 700684 and susceptible J99 strain, as well as clinical, multidrug-resistant isolate (3CML, resistant to clarithromycin, metronidazole and levofloxacin).

Antibiotic stress affects the secretion and physicochemical features of extracellular vesicles produced by Helicobacter pylori.

Background: Bacterial extracellular vesicles (EVs) may reduce the effectiveness of various antimicrobials; however, the impact of antibiotics on the secretion and properties of EVs produced by Helicobacter pylori has not been established.

Methods: Using clinical H. pylori strains and culture in EV-depleted media, the influence of ¼ × MIC of clarithromycin, metronidazole and levofloxacin on EV features was determined.

Halogenated -Benzylbenzisoselenazolones Efficiently Inhibit Ureolysis .

Inspired by the recognized activity of Ebselen against urease, we optimized the structure of 1,2-benzisoselenazol-3(2)-one to provide potent inhibitors of ureolysis in cells. To achieve this goal, we combined the elongation of the N-substituent of Ebselen from phenyl to benzyl with halogenation of the aromatic fragment. The modifications implemented provided compounds with activities that were several times better compared to that of the lead compound.

Efflux Pumps: A Double-Edged Sword in Antibiotic Resistance and Biofilm Formation.

is a major pathogen associated with various gastric diseases. Despite decades of research, the treatment of remains challenging. One of the primary mechanisms contributing to failures of therapies targeting this bacterium is genetic mutations in drug target sites, although the growing body of scientific data highlights that efflux pumps may also take part in this process.

Galleria mellonella larvae as a model for Helicobacter pylori biofilm formation under antibiotic stress.

Helicobacter pylori is a common Gram-negative bacterium that inhabits the human stomach and causes a variety of gastric pathologies. One of the growing concerns is its dynamic spread of antibiotic resistance, a process in which biofilm formation is involved. Therefore, it is necessary to find an appropriate, high-throughput research model for the in vivo biofilm development by H.

What Is a Biofilm? Lessons Learned from Interactions with Immune Cells.

Biofilms are unique, multicellular life forms that challenge our understanding of the microbial functioning. The last decades of research on biofilms have allowed us to better understand their importance in the context of both health and various pathologies in the human body, although many knowledge gaps hindering their correct comprehension still exist. Biofilms are classically described as mushroom-shaped structures attached to the substrate; however, an increasing body of evidence shows that their morphology in clinical conditions may differ significantly from that classically presented.

Subinhibitory concentrations of antibiotics affect development and parameters of biofilm.

Introduction: causes chronic gastric diseases in nearly 50% of people around the world. It is suggested that biofilm formation has a pronounced effect on the dynamic resistance spread and recurrence of these infections.

Methods: To mimic the scenario of therapeutic ineffectiveness, we investigated the impact of sub-minimal inhibitory concentrations (sub-MICs) of antibiotics on the development and parameters of biofilms produced by clinical strains.

Optimization of Biofilm Formation in In Vitro Conditions Mimicking Stomach.

is one of the most common bacterial pathogens worldwide and the main etiological agent of numerous gastric diseases. The frequency of multidrug resistance of is growing and the leading factor related to this phenomenon is its ability to form biofilm. Therefore, the establishment of a proper model to study this structure is of critical need.

Antibacterial and antibiofilm agents in the group of xanthone derivatives with piperazine moiety active against drug-resistant Helicobacter pylori strains.

Helicobacter pylori (H. pylori) cause chronic inflammation of the gastric mucosa which can lead to epithelial atrophy and metaplasia resulting in peptic ulcer disease and gastric cancer. The increasing resistance of H.

Vitamin B6 inhibits activity of adenylosuccinate synthetase and growth of reference and clinical, antibiotic-resistant strains.

The current therapies against gastric pathogen are ineffective in over 20% of patients. Enzymes belonging to the purine salvage pathway are considered as novel drug targets in this pathogen. Therefore, the main aim of the current study was to determine the antibacterial activity of pyridoxal 5'-phosphate (PLP), an active form of vitamin B6, against reference and clinical strains of .

Latest Update on Outer Membrane Vesicles and Their Role in Horizontal Gene Transfer: A Mini-Review.

Outer membrane vesicles (OMVs) are spherical, lipid-based nano-structures, which are released by Gram-negative bacteria in both in vitro and in vivo conditions. The size and composition of OMVs depend on not only the producer bacterial species but also cells belonging to the same strain. The mechanism of vesicles' biogenesis has a key role in determining their cargo and the pattern of macromolecules exposed on their surface.

Ether Derivatives of Naringenin and Their Oximes as Factors Modulating Bacterial Adhesion.

Because of the close connection between adhesion and many vital cellular functions, the search for new compounds modulating the adhesion of bacteria belonging to the intestinal microbiota is a great challenge and a clinical need. Based on our previous studies, we discovered that -lkyl naringenin derivatives and their oximes exhibit antimicrobial activity against antibiotic-resistant pathogens. The current study was aimed at determining the modulatory effect of these compounds on the adhesion of selected representatives of the intestinal microbiota: , a commensal representative of the intestinal microbiota, and , a bacterium that naturally colonizes the intestines but has disease-promoting potential.

Inhibitory activity of catecholic phosphonic and phosphinic acids against Helicobacter pylori ureolysis.

Catechols have been reported to be potent covalent inhibitors of ureases, and they exhibit activity by modifying cysteine residues at the entrance to enzymatic active sites. Following these principles, we designed and synthesized novel catecholic derivatives that contained carboxylate and phosphonic/phosphinic functionalities and assumed expanded specific interactions. When studying the chemical stability of the molecules, we found that their intrinsic acidity catalyzes spontaneous esterification/hydrolysis reactions in methanol or water solutions, respectively.

Comparison of antibiofilm activity of low-concentrated hypochlorites vs polyhexanide-containing antiseptic.

Chronic wound infection is highly associated with morbidity and endangers the patient's life. Therefore, wound care products must have a potent antimicrobial and biofilm-eradicating effect. In this work, the antimicrobial/antibiofilm activity of two low-concentrated chlorine-based and releasing solutions was investigated on a total of 78 strains of methicillin-resistant , , and , using the cohesive spectrum of settings, including microtiter plate models, biofilm-oriented antiseptic test, cellulose-based biofilm model, biofilm bioreactors and Bioflux model.

Extracellular Vesicles of Probiotics: Shedding Light on the Biological Activity and Future Applications.

For many decades, the proper functioning of the human body has become a leading scientific topic. In the course of numerous experiments, a striking impact of probiotics on the human body has been documented, including maintaining the physiological balance of endogenous microorganisms, regulating the functioning of the immune system, enhancing the digestive properties of the host, and preventing or alleviating the course of many diseases. Recent research, especially from the last decade, shows that this health-benefiting activity of probiotics is largely conditioned by the production of extracellular vesicles.

Activity of Novel Ultrashort Cyclic Lipopeptides against Biofilm of Isolated from VVC in the Ex Vivo Animal Vaginal Model and BioFlux Biofilm Model-A Pilot Study.

In recent years, clinicians and doctors have become increasingly interested in fungal infections, including those affecting the mucous membranes. Vulvovaginal candidiasis (VVC) is no exception. The etiology of this infection remains unexplained to this day, as well as the role and significance of asymptomatic vaginal colonization.

Biofilm Formation of in Both Static and Microfluidic Conditions Is Associated With Resistance to Clarithromycin.

It is widely accepted that production of biofilm is a protective mechanism against various type of stressors, including exposure to antibiotics. However, the impact of this structure on the spread of antibiotic resistance in is still poorly understood. Therefore, the aim of the current research was to determine the relationship between biofilm formation and antibiotic resistance of .

In Silico Screening and In Vitro Assessment of Natural Products with Anti-Virulence Activity against .

is one of the most frequent human pathogens and a leading etiological agent of various gastric diseases. As stringent response, coordinated by a SpoT protein, seems to be crucial for the survivability of , the main goal of this article was to use in silico computational studies to find phytochemical compounds capable of binding to the active site of SpoT from and confirm the ability of the most active candidates to interfere with the virulence of this bacterium through in vitro experiments. From 791 natural substances submitted for the virtual screening procedure, 10 were chosen and followed for further in vitro examinations.

Antibiofilm and Antimicrobial-Enhancing Activity of and Extracts against Multidrug-Resistant .

is a Gram-negative bacterium that colonizes the stomach of about 60% of people worldwide. The search for new drugs with activity against is now a hotspot in the effective and safe control of this bacterium. Therefore, the aim of this research was to determine the antibacterial activity of extracts from selected plants of the Papaveraceae family against planktonic and biofilm forms of the multidrug-resistant clinical strain of using a broad spectrum of analytical in vitro methods.

Current State of Knowledge about Role of Pets in Zoonotic Transmission of SARS-CoV-2.

Pets play a crucial role in the development of human feelings, social life, and care. However, in the era of the prevailing global pandemic of COVID-19 disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), many questions addressing the routes of the virus spread and transmission to humans are dramatically emerging. Although cases of SARS-CoV-2 infection have been found in pets including dogs, cats, and ferrets, to date there is no strong evidence for pet-to-human transmission or sustained pet-to-pet transmission of SARS-CoV-2.

Myricetin as an Antivirulence Compound Interfering with a Morphological Transformation into Coccoid Forms and Potentiating Activity of Antibiotics against .

, a gastric pathogen associated with a broad range of stomach diseases, has a high tendency to become resistant to antibiotics. One of the most important factors related to therapeutic failures is its ability to change from a spiral to a coccoid form. Therefore, the main aim of our original article was to determine the influence of myricetin, a natural compound with an antivirulence action, on the morphological transformation of and check the potential of myricetin to increase the activity of antibiotics against this pathogen.