The Modulation of Leaf Cell-Wall Composition in Arabidopsis thaliana by the Drought-Mitigating Rhizobacterium, Bacillus endophyticus J13.

Plant growth-promoting rhizobacteria (PGPR) are beneficial soil bacteria that reside near plant roots (in the rhizosphere) and support plants in various ways. The specific molecular mechanisms involved in these beneficial interactions are still under scrutiny. In this context, the present study describes the role of Bacillus endophyticus J13, a multiple abiotic-stress-tolerant PGPR, in modulating various components of the leaf cell wall in Arabidopsis thaliana, under well-watered and drought conditions.

Mechanistic and metabolic exploration of neohesperidin against lung cancer cell lines through ROS-mediated mitochondrial apoptosis: An in-silico and in-vitro approach.

Lung cancer is a significant contributor to global mortality rates in the human population. However, the results of current treatment options are still unsatisfactory. Thus, the study explores low-toxic natural substances that release caspases and trigger apoptosis.

Protein profiling uncovers IGF-1R inhibition potential of 3-(2-furoyl)-indole scaffolds in hepatocellular carcinoma.

Aim: This study investigates the anti-proliferative potential and possible molecular mechanisms of 3-(2-furoyl)-indole derivatives against HepG2.

Method: Identified hit compounds (4a, 4b, 4c) using MTT screening, were further investigated for their efficacy and mechanism of action through FACS studies, in-silico molecular docking, molecular dynamics (MD) simulations, and label-free quantitative proteome and ADMET prediction.

Results: Lead compound 4a, showed IC50 of 27 µM against HepG2 cells and a binding score of -8.

Comparative analysis of plasma affinity depletion methods: Impact on protein composition and phosphopeptide abundance in human plasma.

Affinity-based protein depletion and TiO enrichment methods play a crucial role in detection of low-abundant proteins and phosphopeptides enrichment, respectively. Here, we assessed the effectiveness of HSA/IgG (HU2) and Human 7 (HU7) depletion methods and their impact on phosphopeptides coverage through comparative proteome analysis, utilizing in-solution digestion and nano-LC-Orbitrap mass spectrometry (MS). Our results demonstrated that both HU2 and HU7 affinity depletion significantly decreased high-abundant proteins by 1.