The Modulation of Leaf Cell-Wall Composition in Arabidopsis thaliana by the Drought-Mitigating Rhizobacterium, Bacillus endophyticus J13.

Plant growth-promoting rhizobacteria (PGPR) are beneficial soil bacteria that reside near plant roots (in the rhizosphere) and support plants in various ways. The specific molecular mechanisms involved in these beneficial interactions are still under scrutiny. In this context, the present study describes the role of Bacillus endophyticus J13, a multiple abiotic-stress-tolerant PGPR, in modulating various components of the leaf cell wall in Arabidopsis thaliana, under well-watered and drought conditions.

Arabidopsis GELP53 overexpression modulates polysaccharide acetylation and defense through oligosaccharide-mediated signaling.

O-acetylation is a crucial substitution found in hemicelluloses and pectin, which are necessary for maintaining the flexibility and structural integrity of the cell. Cell wall acetyl transferases and esterases maintain balanced polysaccharide O-acetylation levels, however the role of esterases in cell wall polysaccharide O-acetylation metabolism is not well explored. Therefore, we investigated the role of the Arabidopsis (Arabidopsis thaliana) GDSL Esterase/Lipase Protein (GELP) family member AtGELP53.

Arabidopsis GELP7 functions as a plasma membrane-localized acetyl xylan esterase, and its overexpression improves saccharification efficiency.

Acetyl substitution on the xylan chain is critical for stable interaction with cellulose and other cell wall polymers in the secondary cell wall. Xylan acetylation pattern is governed by Golgi and extracellular localized acetyl xylan esterase (AXE). We investigated the role of Arabidopsis clade Id from the GDSL esterase/lipase or GELP family in polysaccharide deacetylation.