Post-COVID syndrome in symptomatic COVID-19 patients: a retrospective cohort study.

Background: Although post-COVID symptoms have been documented in the literature, the risk factors and time required for full recovery remain unclear. We conducted a retrospective analysis of medical records of COVID-19 patients to investigate the prevalence of symptoms after an acute episode of COVID-19 and the risk factors for persistence of symptoms.

Methods: This retrospective cohort study analysis examined hospital records of post-COVID individuals with previously confirmed or probable SARS-CoV-2 infection and endurring symptom continuation for at least 3 months post-infection or presenting new symptoms persisting for at least 2 months.

Emergence of a carbapenem-resistant atypical uropathogenic Escherichia coli clone as an increasing cause of urinary tract infection.

Carbapenem-resistant Enterobacterales pose a critical global health threat, exemplified by increasing resistance of uropathogenic E. coli (UPEC) that cause urinary tract infections (UTIs). Here, we investigate the publicly available EnteroBase dataset and identify a signal of increasing UTI caused by phylogroup A E.

Covert communication performance evaluation in UAV-assisted rate-splitting multiple access systems.

In this paper, we investigate using rate-splitting multiple access (RSMA) to facilitate covert communication in a multi-user unmanned aerial vehicle (UAV) downlink communication network that is being monitored by a warden (Willie). We establish a comprehensive analytical framework and derive closed-form expressions for key performance metrics under Nakagami-m fading channels. Specifically, we analyze the detection error probability (DEP) at Willie to quantify system covertness, in addition to outage probability (OP) and ergodic rate (ER) experienced by legitimate users, along with asymptotic analysis in the high signal-to-noise ratio (SNR) region.

Screening benefits for breast and colorectal cancer-specific survival among medicare enrollees with HIV.

Objective: Cancer screening improves survival in the general population, but its effectiveness in people with HIV (PWH) remains underexplored, particularly for breast and colorectal cancer. This study examines the impact of cancer screening on cancer-specific survival among U.S.

Precise MRI-histology coregistration of paraffin-embedded tissue with blockface imaging.

Magnetic resonance imaging (MRI) provides 3D spatial information on tissue, yet it lacks at the molecular level. In contrast, histology provides cellular and molecular information, but it lacks the 3D spatial context and direct translation. Coregistering the two is key for the 3D embedding of histological details, validating pathological MRI findings, and identifying quantitative imaging biomarkers of neurodegenerative diseases.

Effects of cetylated fatty acids on knee osteoarthritis: a systematic review and meta-analysis of real-world evidence.

Objectives: Knee osteoarthritis (OA) continues to be a debilitating global health issue, with effective treatments that have minimal side effects still lacking in modern clinical practice. Cetylated fatty acids (CFAs), a group of esterified fatty acids administered either topically or orally to protect the synovial membrane and stabilise cell membranes, appears to be a promising therapeutic option.

Methods: We conducted a comprehensive search in PubMed, Embase, Cochrane Library, CENTRAL, and Web of Science from the inception until November 2024 to examine the therapeutic effects of CFAs.

Polymer Network Architecture of Single Network and Semi-Interpenetrating Network Hydrogels Modulates Water Retention and Degradation in Soil Conditioning Applications.

With the global population projected to reach 9.7 billion by 2050, agricultural systems must address challenges related to soil fertility, water retention, and sustainability. To address these issues, biobased hydrogels made from natural polymers, such as gelatin methacrylate (GelMA) and chitosan (CS), have shown promise as sustainable soil conditioners.

A genetic safeguard for eliminating target genes in synthetic probiotics in a gut environment.

To meet the emerging demand for constraining engineered probiotic activities, many biocontainment studies explore strategies that involve killing engineered microbes, which often create basal levels of cytotoxicity that hamper cell fitness and performance. Here, we explored a circuit design that destroys the engineered genetic materials in a probiotic strain, instead of killing these cells, under non-permissive conditions. Our safeguard circuit involves a two-layered transcriptional regulatory circuit to control the expression of a CRISPR system that targets the engineered genes for degradation.

Integrated Diagnostic Approach Using Basophil Activation Test and IgE Assays for Shrimp and Prawn Allergy.

: Shellfish allergies are common in Vietnam. The basophil activation test (BAT) is a powerful tool in the diagnosis of food allergies. We aimed to evaluate the application of BAT to distinguish shrimp allergy in comparison with skin prick test and specific IgE measurement.

Predicting 14-day readmission in middle-aged and elderly patients with pneumonia using emergency department data: a multicentre retrospective cohort study with a survival machine learning approach.

Objectives: Unplanned pneumonia readmissions increase patient morbidity, mortality and healthcare costs. Among pneumonia patients, the middle-aged and elderly (≥45 years old) have a significantly higher risk of readmission compared with the young. Given that the 14-day readmission rate is considered a healthcare quality indicator, this study is the first to develop survival machine learning (ML) models using emergency department (ED) data to predict 14-day readmission risk following pneumonia-related admissions.

Flagellar switch inverted repeats impact heterogeneity in flagellar gene expression and thus C. difficile RT027/MLST1 virulence.

Clostridioides difficile (C. difficile) RT027 strains cause infections that vary in severity from asymptomatic to lethal, but the molecular basis for this variability is poorly understood. Through comparative analyses of RT027 clinical isolates, we determine that isolates that exhibit greater heterogeneity in their flagellar gene expression exhibit greater virulence in vivo.

Precise MRI-Histology Coregistration of Paraffin-Embedded Tissue with Blockface Imaging.

Magnetic resonance imaging (MRI) provides 3D spatial information on tissue, yet it lacks at the molecular level. In contrast, histology provides cellular and molecular information, but it lacks the 3D spatial context and direct translation. Coregistering the two is key for the 3D-embedding of histological details, validating pathological MRI findings, and finding quantitative imaging biomarkers of neurodegenerative diseases.

Biochemical and molecular characterization of fungal isolates from California annual grassland soil.

Fungi play a pivotal role in ecosystem functionality, driving processes such as decomposition, nutrient cycling, and symbiotic interactions. Their wide enzymatic strategies enable the breakdown of complex organic materials and the valorization of organic waste streams, providing sustainable pathways for bioproduct development. Fungi also exhibit significant potential in industrial applications, particularly in biofuel and nutraceutical production, owing to their high lipid content and adaptability to diverse feedstocks.

Prevalence of common autosomal recessive and X-linked conditions in pregnant women in Vietnam: a cross-sectional study.

The prevalence of recessive disorder carriers among Vietnamese women is still indistinct. This study aims to assess the prevalence of carriers for common autosomal recessive and X-linked conditions among Vietnamese pregnant women and to identify common mutations within these genes. A cross-sectional study was conducted with 8,464 Vietnamese pregnant women with indications for carrier screening tests for recessive disorders from November 2022 to August 2023 at the Institute of DNA Technology and Genetic Analysis.

Deciphering linezolid-induced hematologic toxicity: Targeting TOP2A and TOP2B via its primary metabolite PNU142586.

Linezolid, an oxazolidinone antibiotic, is widely used to treat multidrug-resistant tuberculosis and drug-resistant Gram-positive infections. However, prolonged use is associated with severe hematologic toxicity, the underlying mechanisms of which remain incompletely understood, particularly regarding the role of linezolid metabolites. Our clinical study indicates that elevated exposure to PNU142586, a primary metabolite of linezolid, is associated with an increased risk of linezolid-induced toxicity, even in the absence of renal impairment.

Correction: Comparison of excitation wavelengths in surface-enhanced Raman spectroscopy coupled to thin-layer chromatography for tadalafil and vardenafil detection as adulterants in herbal healthcare products.

Correction for 'Comparison of excitation wavelengths in surface-enhanced Raman spectroscopy coupled to thin-layer chromatography for tadalafil and vardenafil detection as adulterants in herbal healthcare products' by Dao Thi Cam Minh , , 2025, , 2867-2877, https://doi.org/10.1039/D5AY00219B.

Seedling growth rate and root traits in the maize Nested Association Mapping (NAM) panel.

This paper characterizes variation in shoot and root traits collected from the founders of the Maize Nested Association Mapping panel, which was designed to maximize genetic diversity while ensuring appropriate flowering in eastern North America. Here, we present a detailed account of greenhouse experiments conducted by four cohorts of undergraduate research interns at the University of Hawai'i at Mānoa. We summarize data collection, data cleaning procedures, and present data for 38 phenotypic variables for 24 genotypes with the number of plant replicates ranging from 3 to 20.

Quantitative stable isotope probing (qSIP) and cross-domain networks reveal bacterial-fungal interactions in the hyphosphere.

Background: Interactions between fungi and bacteria have the potential to substantially influence soil carbon dynamics in soil, but we have yet to fully identify these interactions and partners in their natural environment. In this study, we stacked two powerful methods, C quantitative stable isotope probing (qSIP) and cross-domain co-occurrence network, to identify interacting fungi and bacteria in a California grassland soil. We used in-field whole plant CO labeling along with sand-filled ingrowth bags (that trap fungi and hyphae-associated bacteria) to amplify the signal of fungal-bacterial interactions, separate from the bulk soil background.

Human Papilloma Virus (HPV)-Mediated Oropharyngeal Squamous Cell Carcinoma Incidence Relative to Geographic Rates of HPV Vaccination in the United States.

Objective This study aims to analyze the trends in the incidence of human papilloma virus (HPV)-mediated oropharyngeal squamous cell carcinoma (OPSCC) in relation to geographic rates of HPV vaccination in the United States.  Methods The US Cancer Statistics (USCS) database and the National Cancer Institute's (NCI's) Surveillance, Epidemiology and End Results (SEER) program were used for incidence and population data collection; the Centers for Disease Control and Prevention's (CDC) Teen National Immunization Survey provided vaccination data. Incidence of HPV-mediated OPSCC and the HPV vaccination rate were compared and analyzed using t-tests, ANOVA analyses, and linear regression analyses.

Legacy of Repeated Cultivation Drives Cyclical Microbial Community Development in a Tropical Oxisol Soil.

Agricultural practices and the crop being actively cultivated are some of the most important contributors to soil microbial community assembly processes in agroecosystems. However, it is not well-understood how the cultivation of diverse crop species can directionally shift complex soil microbial communities, especially under continuous monoculture systems. Here, we conducted a field experiment to assess how three crop species (Lactuca sativa, Brassica juncea, and Zea mays) may shift soil microbial (bacteria/archaea and fungi) communities when planted in a monoculture and repeatedly grown for three cycles in a tropical Oxisol soil.

Description of bla-carrying Escherichia coli in patients from a Brazilian hospital over a 4-year period.

Klebsiella pneumoniae carbapenemase (KPC)-producing Escherichia coli are recognized by the World Health Organization as a critical group of bacterial priority pathogens of public health importance. Thus, increased understanding of the genetic characteristics of KPC-producing E. coli is required.

Prevalence and associated factors of pediatric uncontrolled asthma in Vietnam.

Objective: Uncontrolled asthma profoundly affects quality of life and imposes a significant societal burden. However, reports on its prevalence and contributing factors in Vietnam remain limited. This study aimed to address this gap by investigating the prevalence and factors associated with uncontrolled asthma among children in Vietnam.

Programming Nutrient Detection with Modular Regulators for Dynamic Control of Microbial Biosynthesis.

Dynamic control of biosynthetic pathways improves the bioproduction efficiency. One common approach is to use genetic sensors that control pathway expression in response to a nutrient molecule in the target feedstock. However, programming the cellular response requires the engineering of numerous genetic parts, which poses a significant barrier to explore the use of different nutrients as cellular signals.

Impact of deep learning on pediatric elbow fracture detection: a systematic review and meta-analysis.

Objectives: Pediatric elbow fractures are a common injury among children. Recent advancements in artificial intelligence (AI), particularly deep learning (DL), have shown promise in diagnosing these fractures. This study systematically evaluated the performance of DL models in detecting pediatric elbow fractures.