Estimating Dyad Level Processes in MFT Research: General Overview of the Common Fate Model and Extensions Into Mediation, Moderation, Growth, and Hybrid Models.

Traditional statistical methods and models, such as linear regression, are commonly implemented in Couple/Marriage Family Therapy (C/MFT) research, yet these methods are intractable to answer dyadic and systemic questions. In the past 30 years, dyadic analysis has emerged to address such problems, and the actor-partner interdependence model (APIM) is seemingly the default analysis. The APIM answers research questions related to mutual influence, but the APIM cannot answer questions at the dyad level (e.

Reasons for Separation and Divorce, Coparenting, and Child Behavioral and Emotional Difficulties: A Common Fate Analysis.

Despite reasons for separation and divorce being critical to understanding coparenting and children's adjustment, they are often overlooked risk factors. Additionally, by incorporating both partners' perspectives, dyadic data help us fully understand the interplay between reasons for separation/divorce, coparenting, and child adjustment. Using data from 926 separating or divorcing heterosexual couples in a coparenting educational program, we explore the indirect effects from partners' shared perceptions of two common reasons for divorce (family violence and parenting differences) to children's behavioral and emotional problems via coparenting, utilizing the common fate mediation model.

Covalent inhibition of Ubc13 impairs global protein synthesis.

The ubiquitin-conjugating enzyme 13 (Ubc13) has an essential function and putative role in artemisinin activity against . Ubc13 conjugates lysine 63-linked ubiquitin (K63-Ub) to proteins, but the role of this modification in remains largely unknown. Herein, we characterize and deploy NSC697923 to interrogate PfUbc13 function.

Extrinsic and intrinsic factors affect copper-induced protein precipitation across eukaryotic and prokaryotic proteomes.

The susceptibility of a protein to aggregation upon exposure to copper ions (Cu) has been recognized as a contributor to Cu-induced cellular dysfunction and toxicity. Different cell types succumb to Cu to varying degrees, indicating innate differences between species in the mechanisms used to tolerate exposure to Cu in excess of their biological needs. Investigated here are properties associated with metal-induced protein precipitation (MiPP) compared across cell lysates generated from three cell lines from three different species: Escherichia coli, Candida albicans, and the human prostate cancer cell line 22Rv1.

Experimental and on-sky demonstration of spectrally dispersed wavefront sensing using a photonic lantern.

Adaptive optics (AO) systems are critical in any application where highly resolved imaging or beam control must be performed through a dynamic medium. Such applications include astronomy and free-space optical communications, where light propagates through the atmosphere, as well as medical microscopy and vision science, where light propagates through biological tissues. Recent works have demonstrated common-path wavefront sensors (WFSs) for adaptive optics using the photonic lantern (PL), a slowly varying waveguide that can efficiently couple multi-moded light into single-mode fibers (SMFs).

A longitudinal examination into childhood abuse and substance use to PTSD symptoms and deviant peer association: Impact of developmental timing of abuse.

Background: Following child abuse, adolescents often endorse PTSD symptoms and use substances. However, few studies have addressed the longitudinal underlying effects of deviant peer association and revictimization and the developmental impact of PTSD symptoms and substance use.

Objective: To address these limitations, the current study utilized the susceptibility and self-medication hypothesis to examine abuse in childhood ages 0-12 and adolescence ages 12-16 as predictors of substance use in mid-adolescence at age 16 and symptoms of (PTSD) in late adolescence at age 18 mediated by associating with deviant peers and revictimization using a sample of 596 (73.

Small molecules reveal differential shifts in stability and protein binding for G-quadruplex RNA.

The potential of therapeutically targeting RNA with small molecules continues to grow yet progress is hindered by difficulties in determining specific mechanisms of action, including impacts on RNA-protein binding. RNA G-quadruplexes (rGQs) are a particularly promising target due to their range of biological functions, structural stability, and hydrophobic surfaces, which promote small molecule and protein interactions alike. Challenges arise due to 1) the low structural diversity among rGQs, thereby limiting binding selectivity, and 2) a lack of knowledge regarding how small molecules can manipulate rGQ-protein binding on a global scale.

Albumin-based delivery systems: Recent advances, challenges, and opportunities.

Albumin and albumin-based biomaterials have been explored for various applications, including therapeutic delivery, as therapeutic agents, as components of tissue adhesives, and in tissue engineering applications. Albumin has been approved as a nanoparticle containing paclitaxel (Abraxane®), as an albumin-binding peptide (Victoza®), and as a glutaraldehyde-crosslinked tissue adhesive (BioGlue®). Albumin is also approved as a supportive therapy for various conditions, including hypoalbuminemia, sepsis, and acute respiratory distress syndrome (ARDS).

Evaluating the Impacts of Community-Campus Engagement on Population Health in Ottawa and Thunder Bay, Canada: Protocol for a Mixed Methods Contribution Analysis.

Background: Municipalities play a crucial role in population health due to their community connections and influence on health determinants. Community-campus engagement (CCE), that is, collaboration between academic institutions and communities, is a promising approach to addressing community health priorities. However, evidence of CCE's impact on population health remains limited.

Integrative multiomics reveals common endotypes across PSEN1, PSEN2, and APP mutations in familial Alzheimer's disease.

Background: PSEN1, PSEN2, and APP mutations cause Alzheimer's disease (AD) with an early age at onset (AAO) and progressive cognitive decline. PSEN1 mutations are more common and generally have an earlier AAO; however, certain PSEN1 mutations cause a later AAO, similar to those observed in PSEN2 and APP.

Methods: We examined whether common disease endotypes exist across these mutations with a later AAO (~ 55 years) using hiPSC-derived neurons from familial Alzheimer's disease (FAD) patients harboring mutations in PSEN1, PSEN2, and APP and mechanistically characterized by integrating RNA-seq and ATAC-seq.

Discovery of a Pseudomonas aeruginosa-specific small molecule targeting outer membrane protein OprH-LPS interaction by a multiplexed screen.

The surge of antimicrobial resistance threatens efficacy of current antibiotics, particularly against Pseudomonas aeruginosa, a highly resistant gram-negative pathogen. The asymmetric outer membrane (OM) of P. aeruginosa combined with its array of efflux pumps provide a barrier to xenobiotic accumulation, thus making antibiotic discovery challenging.

The association of combinations of social factors and SARs-CoV-2 infection: A retrospective population-based cohort study in Ontario, 2020-2021.

Objective: The COVID-19 pandemic highlighted and exacerbated health inequities worldwide. While several studies have examined the impact of individual social factors on COVID infection, our objective was to examine how interactions of social factors were associated with the risk of testing positive for SARS-CoV-2 during the first two years of the pandemic.

Study Design And Setting: We conducted an observational cohort study using linked health administrative data for Ontarians tested for SARS-CoV-2 between January 1st, 2020, and December 31st, 2021.

Nonlinear techniques for few-mode wavefront sensors.

We present several nonlinear wavefront sensing techniques for few-mode sensors, all of which are empirically calibrated and agnostic to the choice of wavefront sensor. The first class of techniques involves a straightforward extension of the linear phase retrieval scheme to higher order; the resulting Taylor polynomial can then be solved using the method of successive approximations, though we discuss alternate methods such as homotopy continuation. In the second class of techniques, a model of the WFS intensity response is created using radial basis function interpolation.

Top-Down Stability of Proteins from Rates of Oxidation (TD-SPROX) Approach for Measuring Proteoform-Specific Folding Stability.

The crucial roles of proteoforms in biological processes and disease mechanisms have been increasingly recognized. However, the rate at which new proteoforms are being discovered using top-down proteomics has far outpaced the rate at which the functional significance of different proteoforms can be determined. Because of the close connection between protein folding and protein function, protein folding stability measurements on proteoforms have the potential to identify functionally significant proteoforms of a given protein.

Complications and Outcomes of Bone-Anchored Prostheses of the Hand: A Systematic Review.

Purpose: The purpose of this study was to conduct a systematic review evaluating the reported complications and outcomes of bone-anchored prostheses in digit and partial hand amputees.

Methods: A literature review of primary research articles on osseointegration and bone-anchored prostheses for digit and partial amputees was performed. The Medline, Embase, Scopus, and Cochrane Library databases were queried.

Challenging the use of the overall adverse childhood experiences (ACEs) score: Comparing total ACEs, maltreatment, and household dysfunction on mental health problems among White, African American, and Native American women under correctional control.

Adverse childhood experiences (ACEs) have been consistently linked to mental health problems. There have been recent conceptual and empirical critiques that suggest maltreatment and household dysfunction to not be combined to create a composite ACE score. Women in correctional custody demonstrate disproportionately high ACE levels and greater mental health problems as to racial minorities.

Conceptualizing and measuring childhood adversity: A comprehensive critique of the adverse childhood experiences measure and offering a new conceptualization of childhood adversity.

The landmark adverse childhood experiences (ACEs) study conducted by Kaiser Permanente and the Centers for Disease Control shook the bedrock of the scientific community, highlighting the commonality of ACEs and identifying a dose-response relationship with poor health outcomes. The seminal findings led to a surge in ACEs research and a growing body of empirical literature; however, the ACEs measure has numerous conceptual and measurement issues that are often overlooked in the research. Such problems include a lack of a clear conceptual definition of what constitutes an ACE, item formulation and coverage, item scoring, and lack of contextual information.

Analysis of Brain Protein Stability Changes in a Mouse Model of Alzheimer's Disease.

The stability of proteins from rates of oxidation (SPROX), thermal proteome profiling (TPP), and limited proteolysis (LiP) techniques were used to profile the stability of ∼2500 proteins in hippocampus tissue cell lysates from 2- and 8-months-old wild-type (C57BL/6J; = 7) and transgenic (5XFAD; = 7) mice with five Alzheimer's disease (AD)-linked mutations. Approximately 200-500 protein hits with AD-related stability changes were detected by each technique at each age point. The hit overlap from technique to technique was low, and all of the techniques generated protein hits that were more numerous and largely different from those identified in protein expression level analyses, which were also performed here.

The Use of Contribution Analysis in Evaluating Health Interventions: A Scoping Review.

Contribution Analysis (CA) is a promising theory-based evaluation approach for complex interventions, yet its application in health interventions remains largely unexplored. To bridge this gap, we conducted a scoping review to examine the extent of such applications and the methodologies, strengths, and limitations of this approach in health programming. Our comprehensive search strategy was developed and used in 15 databases to identify peer-reviewed articles from 1999 to 2023 that focused on using CA to evaluate health interventions.

Childhood sexual abuse and IL6 mediated by change in BMI over an 18-year period: A growth curve model.

Background: Childhood sexual abuse can increase both body weight and inflammation later in life. Higher weight or faster changes in weight, as measured by changes in body mass index (BMI), may mediate the relationship between childhood sexual abuse and inflammation, however, most studies to date have used a cross-sectional design limiting causal inferences.

Objective: The current study aimed to investigate the interrelationships between childhood sexual abuse, BMI, and C-reactive protein (CRP) and interleukin-6 (IL6).

The transition from physical sibling abuse to bullying perpetration through trait anger: A longitudinal study.

Objectives: Experiencing physical sibling abuse is a form of family violence that is common but understudied. While it is often perceived as a normative aspect of sibling relationships, there are apparent behavioral consequences. The current study aims to advance the literature by utilizing the displaced aggression model and I theory to longitudinally examine trait anger as a pathway linking physical sibling abuse to bullying perpetration.

Childhood sexual abuse and cholesterol risk: Testing body mass index as a mediator across gender in a national sample of adults.

Background: Childhood sexual abuse (CSA) is associated with health problems, including cardiometabolic outcomes. Findings directly linking CSA to cholesterol levels are mixed, and identifying mediating pathways is the next logical step. Body mass index (BMI) is one possible mediator, given its association with both CSA and cardiometabolic outcomes.

Generation of 'semi-guided' cortical organoids with complex neural oscillations.

Temporal development of neural electrophysiology follows genetic programming, similar to cellular maturation and organization during development. The emergent properties of this electrophysiological development, namely neural oscillations, can be used to characterize brain development. Recently, we utilized the innate programming encoded in the human genome to generate functionally mature cortical organoids.

"Multiplexed screen identifies a -specific small molecule targeting the outer membrane protein OprH and its interaction with LPS".

The surge of antimicrobial resistance threatens efficacy of current antibiotics, particularly against , a highly resistant gram-negative pathogen. The asymmetric outer membrane (OM) of combined with its array of efflux pumps provide a barrier to xenobiotic accumulation, thus making antibiotic discovery challenging. We adapted PROSPECT , a target-based, whole-cell screening strategy, to discover small molecule probes that kill mutants depleted for essential proteins localized at the OM.