An integrative multi-omics analysis of histone modifications and DNA methylation reveals the epigenomic landscape in apple under drought stress.

Epigenetic regulation plays a key role in plant development and stress response processes. Although previous studies have found that epigenetic modifications are involved in the drought response in apple, a comprehensive epigenomic profile of the response of apple to drought is needed. To characterize epigenomic profiles during the response of apple to drought, we conducted transcriptome, whole-genome bisulfite sequencing, and ChIP-seq analyses of six histone modifications (H3ac, H3K9ac, H3K14ac, H3K4me3, H3K27me3, and H3K36me3) in Malus hupehensis at 0, 3, 6, and 9 days after drought treatment.

Guizhitongluo Tablet inhibits atherosclerosis and foam cell formation through regulating Piezo1/NLRP3 mediated macrophage pyroptosis.

Background: Atherosclerosis (AS) is the main pathological basis for the development of cardiovascular diseases. Vascular inflammation is an important factor in the formation of AS, and macrophage pyroptosis plays a key role in AS due to its unique inflammatory response. Guizhitongluo Tablet (GZTLT) has shown clinically effective in treating patients with AS, but its mechanism is elusive.

Rapid mechanochemical synthesis of high-performance NaFeAl(PO)(PO)/C cathode material for sodium-ion storage.

NaFe(PO)(PO) is regarded as a promising cathode material for sodium-ion batteries due to its affordability, non-toxic nature, and excellent structural stability. However, its electrochemical performance is hampered by its poor electronic conductivity. Meanwhile, most of the previous studies utilized spray-drying and sol-gel methods to synthesize NaFe(PO)(PO), and the large-scale synthesis of the cathode material is still challenging.

Self-Supporting Mn-RuO Nanoarrays for Stable Oxygen Evolution Reaction in Acid.

Currently, the process of an acidic oxygen evolution reaction (OER) necessitates the use of Iridium dioxygen (IrO), which is both expensive and incredibly scarce on Earth. Ruthenium dioxygen (RuO) offers high activity for acidic OERs and presents a potential substitution for IrO. Nevertheless, its practical application is hindered by its relatively poor stability.

HISTONE DEACETYLASE 6 interaction with ABSCISIC ACID-INSENSITIVE 5 decreases apple drought tolerance.

Understanding the molecular regulation of plant response to drought is the basis of drought-resistance improvement through molecular strategies. Here, we characterized apple (Malus × domestica) histone deacetylase 6 (MdHDA6), which negatively regulates apple drought tolerance by catalyzing deacetylation on histones associated with drought-responsive genes. Transgenic apple plants over-expressing MdHDA6 were less drought-tolerant, while those with down-regulated MdHDA6 expression were more drought-resistant than nontransgenic apple plants.

Cell factory-based milk protein biomanufacturing: Advances and perspectives.

The increasing global population and protein demand cause global challenges for food supply. Fueled by significant developments in synthetic biology, microbial cell factories are constructed for the bioproduction of milk proteins, providing a promising approach for scalable and cost-effective production of alternative proteins. This review focused on the synthetic biology-based microbial cell factory construction for milk protein bioproduction.

Integrating ATAC-seq and RNA-seq Reveals the Dynamics of Chromatin Accessibility and Gene Expression in Apple Response to Drought.

Drought resistance in plants is influenced by multiple signaling pathways that involve various transcription factors, many target genes, and multiple types of epigenetic modifications. Studies on epigenetic modifications of drought focus on DNA methylation and histone modifications, with fewer on chromatin remodeling. Changes in chromatin accessibility can play an important role in abiotic stress in plants by affecting RNA polymerase binding and various regulatory factors.

The impact of avoidant attachment on marital satisfaction of Chinese married people: Multiple mediating effect of spousal support and coping tendency.

In this study, the relationship between attachment avoidance and marital satisfaction of Chinese married people as well as the multiple mediating role of spousal support and coping tendency were explored. A model was developed using data of a sample of 510 Chinese married people. Four scales (the Experience of Close Relationships Scale, the Support Scale in Intimate Relationships, the Simple Coping Style Scale, and the Olson Marital Quality Questionnaire) were used to assess attachment avoidance, spousal support, coping tendency, and marital satisfaction, respectively.

Efficient Bioproduction of Human Milk Alpha-Lactalbumin in .

Alpha-lactalbumin (α-LA; the most abundant whey protein in human milk) contributes to infant development, providing bioactive peptides and essential amino acids. Here, () was selected as the production host. We found that the host X33 was suitable for expressing the target protein, yielding 5.

Periodontal ligament stem cells in the periodontitis niche: inseparable interactions and mechanisms.

Periodontitis is characterized by the periodontium's pathologic destruction due to the host's overwhelmed inflammation to the dental plaque. The bacterial infections and subsequent host immune responses have shaped a distinct microenvironment, which generally affects resident periodontal ligament stem cells (PDLSCs). Interestingly, recent studies have revealed that impaired PDLSCs may also contribute to the disturbance of periodontal homeostasis.

Overexpression of microRNA-221 promotes the differentiation of stem cells from human exfoliated deciduous teeth to neurons through activation of Wnt/β-catenin pathway via inhibition of CHD8.

microRNAs have been proved to function in some processes of differentiation and the effect is favorable. At present, the differentiation of stem cells is not so ideal because of the high expenses and inaccessibility. Therefore, we explored the possibility that microRNA-221 (miR-221) affects differentiation from stem cells from human deciduous tooth (SHEDs) to neurons through Wnt/β-catenin pathway via binding to CHD8.

Genome-wide association study reveals novel loci associated with body size and carcass yields in Pekin ducks.

Background: Pekin duck products have become popular in Asia over recent decades and account for an increasing market share. However, the genetic mechanisms affecting carcass growth in Pekin ducks remain unknown. This study aimed to identify quantitative trait loci affecting body size and carcass yields in Pekin ducks.