Auxiliary activity family 9 (AA9) lytic polysaccharide monooxygenases (LPMOs) are monocopper enzymes that oxidatively degrade a variety of polysaccharides. Despite extensive research on this class of enzymes, the essential LPMO gene involved in pathogenicity and its characterization still requires further study. Here, we obtained the candidate effector AA9 LPMOs gene CcAA9-20333 by mining transcriptome data of Colletotrichum coccodes, the causal agent of potato anthracnose.
View Article and Find Full Text PDFThe AA9 LMPO protein, CcAA9-20333, acts as a secretory effector by inhibiting N. benthamiana cell death via its enzymatic activity and contributes to the virulence of C. coccodes.
View Article and Find Full Text PDFRecently, a new bacterial disease was detected on cucumber stalks. In order to study the pathogenesis of this disease, the pathogenic bacteria were isolated and identified on the basis of morphological and molecular characteristics, and further analyzed for pathogenicity and antagonistic evaluation. Pathogenicity analysis showed that HlJ-3 caused melting decay and cracking in cucumber stems, and the strain reisolated from re-infected cucumber stalks was morphologically identical to HlJ-3 colonies, which is consistent with the Koch's postulates.
View Article and Find Full Text PDFFront Microbiol
November 2024
Pectate lyases (PL), as important polysaccharide lyases, play an important role in the infection of host plants by pathogenic. A previous study found that the PL gene was up-regulated in the interaction between 5T-1 and potatoes. In this study, 5T-1 was used as the study object, and its gene function was investigated using bioinformatics analysis, prokaryotic expression, and CRISPR-Cas9 technology.
View Article and Find Full Text PDFAppl Biochem Biotechnol
August 2024
The plant disease Colletotrichum coccodes, which lowers potato yields, poses a severe danger to the booming potato industry. Isolated plant endophytic bacteria from highland pasture can produce a variety of metabolites that lessen the risk that the pathogen C. coccodes poses to plant growth and development.
View Article and Find Full Text PDFBiotic stress deleteriously affects growth, development, and productivity in plants. Proline (Pro) plays a significant role in enhancing plant resistance to pathogen infection. However, its effects on reducing Lelliottia amnigena-induced oxidative stress in potato tubers remain unknown.
View Article and Find Full Text PDFMicrob Pathog
November 2022
Tussilago farfara is of vital medical value. A new leaf spot disease was observed on T. farfara leaves, in Dingxi, Gansu Province, China, in October 2019.
View Article and Find Full Text PDFMicrob Pathog
February 2022
Potato scab caused by pathogenic Streptomyces is a serious soil-borne disease on potato. In this study, a new Streptomyces strain 5A-1 was isolated from potato samples in China. Based on morphological characteristics, 16S rDNA gene sequence analyses, it was identified as Streptomyces griseoplanus (Streptacidiphilus griseoplanus), pathogenicity of which was measured by the methods of small potato chips, radish slices and potato pot trial inoculation.
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