Effect of Ultrasonic Treatment on the Quality of Pumpkin Juice Fermented by Yeast.

To investigate the effect of ultrasound on the quality of pumpkin juice fermented by yeast, ultrasound (power range 0-400 W, duration 10 min) was used to assist the yeast fermentation of pumpkin juice. The effects of ultrasound-assisted fermentation at different powers on the quality characteristics of pumpkin juice, such as the color, aroma components, carotenoid content, and antioxidant activity, were studied. The optimal combination of fermentation process parameters was determined as follows: a pumpkin juice content of 20 mL, fermentation temperature of 30 °C, fermentation time of 1 day, and inoculation amount of 3% (the mass-to-volume ratio of yeast to water).

Quantitative Analysis of Trade Position Shifts of China and the United States in the Indian Ocean Rim Trade Networks Using a Weighted Centrality Approach.

The Indian Ocean Rim (IOR) is a crucial hub for global commerce, possessing key maritime corridors and competitive markets for China and the United States. Assessing the evolving positions of China and the United States in regional trade provides critical insights into their economic competition. This study quantitatively investigated their changing positions in the IOR trade networks from 1992 to 2020 through an interdisciplinary approach combining the Fisher optimal segmentation, chord-diagram visualization, and five weighted centrality indicators, including two advanced metrics derived from physical current flow theory.

Dynamic effects of ultrasonic treatment on flavor and metabolic pathway of pumpkin juice during storage based on GC-MS and GC-IMS.

In this study, the dynamic effects of ultrasonic treatment (0-400 W) on the volatile flavor compounds of pumpkin juice under different storage periods were investigated systematically using a combination of headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) and gas chromatography-ion mobility spectrometry (GC-IMS) techniques. A total of 139 and 46 volatile organic compounds (VOCs) were identified by GC-MS and GC-IMS, respectively. The results indicated that complex changes in volatile components occurred during storage.

Influence of ultrasound on the microbiological, physicochemical properties, and sensory quality of different varieties of pumpkin juice.

This study has investigated the effect of ultrasound (US) as an emerging non-thermal sterilization technique on microbial growth and quality changes in three freshly squeezed pumpkin juices ( Duchesne, Duchesne, and L.).The three pumpkin juices were ultrasonicated at different ultrasonic power (0-400 W), time (0-20 min), and temperature (0-30 °C), and the total colony counts of the treated pumpkin juices were less than 5 log CFU/mL, which complied with the food safety and consumption standards.

Hybridization chain reaction and DNAzyme-based dual signal amplification strategy for sensitive fluorescent sensing of aflatoxin B1 by using the pivot of triplex DNA.

This work developed an enzyme-free fluorescent aptasensor for sensitive aflatoxin B1 (AFB1) detection based on a dual signal amplification strategy of hybridization chain reaction (HCR) and Zn-dependent DNAzyme. In the presence of AFB1, the aptamer specifically binds to the target, releasing the blocking DNA, which can initiate HCR between hairpin probes H1 and H2. With the addition of the substrate strand (Zn-Sub) and enzyme strand (Zn-Enz) of DNAzyme, HCR product can hybridize with Zn-Sub and Zn-Enz to form triplex DNA and Y-shaped structure together, which further activates the DNAzyme to cleave Zn-Sub.

Room temperature monitoring of SFdecomposition byproduct SOFbased on TiO/NiSOcomposite nanofibers.

Sulfuryl fluoride (SOF) is one of the ideal decomposition components of sulfur hexafluoride (SF), which is widely used as an insulating and arc extinguishing medium in gas-insulated switchgear. To detect the decomposition component of SFat room temperature, the use of SOFis still a challenge. In this work, we have successfully fabricated TiOnanofibers and nickel sulfate (NiSONPs) via simple electrospun and hydrothermal methods, followed by calcination process to improve the sensing performance.

A novel ethanol gas sensor based on α-BiMoO/CoO nanotube-decorated particles.

A novel composite based on α-BiMoO/CoO nanotube-decorated particles was successfully synthesized using a highly efficient and facile two step system using electrospinning and hydrothermal techniques. The small size CoO nanoparticles were uniformly and hydrothermally developed on the electrospun α-BiMoO nanotubes. The pure α-BiMoO nanofibers and composite based on α-BiMoO/CoO were examined using X-ray powder diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), high resolution transmission electron microscopy (HRTEM), X-ray photoelectron spectroscopy (XPS) and Brunauer-Emmett-Teller (BET) analyses.

Development of high-performance sensor based on NiO/SnO heterostructures to study sensing properties towards various reducing gases.

In this work, we report the spontaneous formation of NiO nanoparticles-decorated onto smooth SnO nanofibers, which is an inexpensive and scalable method for yielding a high composite surface area via a simple two-step synthesis process based on electrospinning and the hydrothermal method. A Nickel Oxide proton-conducting electrolyte is deposited homogeneously over a large surface area in a transparent solution, mixed and decorated onto Tin dioxide nanofibers, as evidenced by cross sectional imaging of the electrospun nanofibers. The composite based on nanoparticle-decorated fibers enlarges the surface area of the exposed electrolyte, which fundamentally improves the overall gas sensing performance.

A novel self-healing power cable insulating material based on host-guest interactions.

The insulating materials used in power cables are susceptible to damage and cracks during installation and operation. To solve this problem, we have prepared a self-healing material PVP/p(HEMA--BA), which is synthesized by radical polymerization using HEMA, BA, PVP and a host-guest assembly. The host-guest assembly is constructed through interactions between host and guest molecules (CD-AlO NPs act as the host, and HEMA-Ad acts as the guest).

Sequence specific recognition of HIV-1 dsDNA in the large amount of normal dsDNA based upon nicking enzyme signal amplification and triplex DNA.

A sensitive fluorescent strategy for sequence specific recognition of HIV dsDNA was established based upon Nicking Enzyme Signal Amplification (NESA) and triplex formation. dsDNA sequence from the site 7960 to site 7991 of the HIV1 dsDNA gene was designed as target dsDNA, which was composed of two complementary strands Oligonucleotide 1 with the sequence of 3'-CTT CCT TAT CTT CTT CTT CCA CCT CTC TCT CT-5' (Oligo-1) and Oligonucleotide 2 with the sequence of 5'-GAA GGA ATA GAA GAA GAA GGT GGA GAG AGA GA-3' (Oligo-2). As a proof of concept, Oligonucleotide 5'-6-FAM-GAG GTG GAG CTG CGC GAC TCC TCC TCT CTC TCT CTC CAC CTC-BHQ-1-3'(Oligo-4) acted as molecular beacon(MB) probe, Oligonucleotide 5'-CTT CCT TAT CTT CTT CTT CCA AAA GGA GTC GCG-3' (Oligo-7) acted as assistant probe.

Homogenous assay for protein detection based on proximity DNA hybridization and isothermal circular strand displacement amplification reaction.

This work proposed a homogenous fluorescence assay for proteins, based on the target-triggered proximity DNA hybridization in combination with strand displacement amplification (SDA). It benefited from target-triggered proximity DNA hybridization to specifically recognize the target and SDA making recycling signal amplification. The system included a molecular beacon (MB), an extended probe (EP), and an assistant probe (AP), which were not self-assembly in the absence of target proteins, due to the short length of the designed complementary sequence among MB, EP, and AP.

Ultrasensitive colorimetric detection of circulating tumor DNA using hybridization chain reaction and the pivot of triplex DNA.

This work presents an amplified colorimetric biosensor for circulating tumor DNA (ctDNA), which associates the hybridization chain reaction (HCR) amplification with G-Quadruplex DNAzymes activity through triplex DNA formation. In the presence of ctDNA, HCR occurs. The resulting HCR products are specially recognized by one sequence to include one GGG repeat and the other containing three GGG repeats, through the synergetic effect of triplex DNA and asymmetrically split G-Quadruplex forming.

A colorimetric sensing platform based upon recognizing hybridization chain reaction products with oligonucleotide modified gold nanoparticles through triplex formation.

A novel colorimetric sensing strategy for biomolecule assay has been developed, which integrates the signal amplification of the hybridization chain reaction (HCR) with the assembly of gold nanoparticles (AuNPs) through triplex formation. In the presence of targets, the HCR process can be triggered, the HCR products are specifically recognized by triplex-forming oligonucleotide (TFO) functionalized AuNPs, accompanying the aggregation of AuNPs and a dramatic absorbance change at 522 nm. In addition, the DNA hairpin probes can form rigid triplex structures with TFO-functionalized AuNPs in the absence of targets, resulting in a negligible background signal.

Synthesis and spectroscopic characterization of water-soluble fluorescent ag nanoclusters.

Water-soluble fluorescent Ag nanoclusters (NCs) were synthesized at room temperature with sodium dodecyl sulfonate (SDS) as a protective agent. The effects of synthetic conditions on the fluorescence properties of Ag NCs were investigated. The results show that the fluorescence intensity of Ag NCs strongly depends on the synthetic conditions, such as the molar ratio of AgNO3 versus SDS and sodium borohydride (NaBH4), the reaction time, and the pH value of the reaction solution.

Sensitive detection of H2O2 and H2O2-related reactant with Ru(bipy)(2)(7,8-dimethyl-dipyridophenazine)2+ and oligodeoxyribonucleotide.

A sensor for H(2)O(2) and H(2)O(2)-related reactant was constructed with oligonucleotides and Ru(bipy)(2)dppx(2+) (bipy = 2,2'-bipyridine, dppx = 7,8-dimethyl-dipyridophenazine), which was performed by converting the H(2)O(2)-induced DNA cleavage into the change of luminescence. The 'DNA light switch' Ru(bipy)(2)dppx(2+) could emit strong luminescence in the presence of dsDNA. DNA cleavage occurred upon addition of H(2)O(2) due to the Fenton reaction, which resulted in the decrease of the luminescence of Ru(bipy)(2)dppx(2+).