A B3 Domain Transcription Factor NtMAB1 Regulates the Release and Outgrowth of Axillary Buds in Tobacco.

The release and outgrowth of axillary buds are essential parts of the process of shoot branching in plants, but knowledge of the underlying regulatory mechanisms remains limited. Analysis of mab1-D, a tobacco mutant with a greater number of axillary branches, showed that the mutant phenotype resulted from positive modulation of axillary buds release and outgrowth. TAIL-PCR and co-separation analyses suggested that NtMAB1-S1, a member of the Related to ABI3/VP1 (RAV) subfamily that was highly expressed in the base of axillary buds, was associated with the mutant phenotype.

The IbDof2.1-IbABF2 module regulates abscisic acid responses and proline biosynthesis to enhance drought tolerance in sweetpotato.

Drought is a major abiotic stress that impairs plant growth and development. Developing drought-tolerant crop varieties is an important goal of breeders. Transcription factors belonging to the DNA-binding with one zinc finger (Dof) family regulate plant stress responses and development.

Natural allelic variation of NAC transcription factor 22 regulates starch biosynthesis and properties in sweetpotato.

Sweetpotato (Ipomoea batatas) starch is in high demand globally as a food and industrial product. However, the regulatory mechanisms governing starch biosynthesis and starch properties in this important crop remain largely unknown. Here we identified a natural allelic variant in the promoter of IbNAC22, encoding a NAC (NAM, ATAF1/2, and CUC2) transcription factor, which is closely linked to starch content in sweetpotato.

Dimethyl carbonate replaces xylene in paraffin section as a biosafe and effective transparent reagent.

Paraffin section is an important technique in histology research, and transparency is the key step connecting the procedures of dehydration and wax immersion in paraffin sectioning technology. However, the commonly used transparent reagent xylene is toxic to the human body and the environment and included in the list of carcinogens. Few biosafe and environmentally friendly transparent reagents have been widely used to replace xylene.

Genome-wide identification of SWEET family genes and functional analysis of NtSWEET12i under drought and saline-alkali stresses in tobacco.

Background: SWEET (Sugar Will Eventually be Exported Transporter) proteins play vital roles in the transport of sugars, contributing to the regulation of plant development, hormone signaling, and responses to abiotic stress.

Results: In this study, we identified 57 NtSWEETs in tobacco (Nicotiana tabacum L.), and then their physicochemical properties, chromosomal localization, synteny, phylogenetic relationships, genomic structure, promoter cis-elements, protein interaction network, tissue specificity, and expression pattern were systematically analyzed.

Genome-wide identification and analysis of the NF-Y transcription factor family reveal its potential roles in tobacco ( L.).

Nuclear Factor Y (NF-Y) represents a group of transcription factors commonly present in higher eukaryotes, typically consisting of three subunits: NF-YA, NF-YB, and NF-YC. They play crucial roles in the embryonic development, photosynthesis, flowering, abiotic stress responses, and other essential processes in plants. To better understand the genome-wide NF-Y domain-containing proteins, the protein physicochemical properties, chromosomal localization, synteny, phylogenetic relationships, genomic structure, promoter -elements, and protein interaction network of NtNF-Ys in tobacco ( L.

"Mending with silk" enhances aged silk with mechanical and antibacterial properties.

The reinforcement and preservation of historical silk are crucial for its continued research, heritage, and display. Herein, silk fibroin (SF) and carboxymethyl chitosan (CMCS) were utilized as raw materials to reinforce aged silk via two reinforcement methods, with transglutaminase (TGase) serving as a catalytic cross-linking agent. The covalent and non-covalent bond network formed by TGase catalytic cross-linking significantly improved the mechanical properties of aging silk, and CMCS as an antibacterial material gave excellent antibacterial properties of the aged silk.

IbNF-YA1 is a key factor in the storage root development of sweet potato.

As a major worldwide root crop, the mechanism underlying storage root yield formation has always been a hot topic in sweet potato [Ipomoea batatas (L.) Lam.].

Contributes to Salt and Drought Stress Tolerance via Modulating Anthocyanin Accumulation and ROS-Scavenging System in Sweet Potato.

Basic helix-loop-helix (bHLH) transcription factors extensively affect various physiological processes in plant metabolism, growth, and abiotic stress. However, the regulation mechanism of bHLH transcription factors in balancing anthocyanin biosynthesis and abiotic stress in sweet potato ( (L.) Lam.

The IbPYL8-IbbHLH66-IbbHLH118 complex mediates the abscisic acid-dependent drought response in sweet potato.

Drought limits crop development and yields. bHLH (basic helix-loop-helix) transcription factors play critical roles in regulating the drought response in many plants, but their roles in this process in sweet potato are unknown. Here, we report that two bHLH proteins, IbbHLH118 and IbbHLH66, play opposite roles in the ABA-mediated drought response in sweet potato.

A cytochrome P450 superfamily gene, IbCYP82D47, increases carotenoid contents in transgenic sweet potato.

The cytochrome P450 superfamily (CYP450) is one of the largest protein families in plants, and its members play diverse roles in primary and secondary metabolic biosynthesis. In this study, the CYP450 family gene IbCYP82D47 was cloned from the high carotenoid line HVB-3 of sweet potato (Ipomoea batatas). The IbCYP82D47 protein harbored two transmembrane domains and dynamically localized between plastid stroma and membrane.

Comorbid Anxiety and Depression and Related Factors Among Pregnant and Postpartum Chinese Women During the Coronavirus Disease 2019 Pandemic.

To identify the prevalence of comorbid anxiety and depression (CAD) and analyze the relationship between CAD and sociodemographic and obstetric-related variables in pregnant and postpartum Chinese women during the COVID-19 pandemic. Participants were 2,237 pregnant and postpartum women (aged 19-47 years) who visited various medical institutions in China between February 28, 2020, and April 26, 2020. They were asked to complete an online survey assessing the anxiety and depression, obstetric characteristics, and sociodemographic variables.

A lycopene β-cyclase gene, IbLCYB2, enhances carotenoid contents and abiotic stress tolerance in transgenic sweetpotato.

Lycopene β-cyclase (LCYB) is an essential enzyme that catalyzes the conversion of lycopene into α-carotene and β-carotene in carotenoid biosynthesis pathway. However, the roles and underlying mechanisms of the LCYB gene in plant responses to abiotic stresses are rarely known. This gene has not been used to improve carotenoid contents of sweetpotato, Ipomoea batatas (L.