Risk-Stratified Pretransplant Immunosuppressive Strategy Improves Outcomes of Unrelated Cord Blood Transplantation in Pediatric β-Thalassemia Major.

Unrelated umbilical cord blood transplantation (UD-UCBT) is a curative option for children with β-thalassemia major (β-TM) who lack HLA-matched sibling donors. However, challenges such as low CD34+ cell dose, pre-existing immune activation, and iron overload contribute to poor engraftment and high transplant-related mortality (TRM) in high-risk patients. To evaluate the safety and efficacy of a novel 5-factor risk-stratified pretransplant immunosuppressive strategy, combined with a myeloablative conditioning regimen featuring "early" reduced-dose anti-thymocyte globulin (ATG), in improving transplant outcomes and immune reconstitution in pediatric patients undergoing UD-UCBT for β-TM.

Upregulating vascular endothelial K2.3 channels alleviates pulmonary hypertension in mice.

Endothelial dysfunction of pulmonary arteries is important in the initiation of pulmonary hypertension (PH). Pulmonary vascular tone is regulated by endothelium-dependent hyperpolarization (EDH) that induces vasodilation. Although K2.

Glutaminolysis and α-ketoglutarate-stimulated K3.1 expression contribute to β-adrenoceptor activation-induced myocardial fibrosis in mice.

Heart failure is associated with myocardial fibrosis, a pivotal histopathological feature arising from β-adrenergic receptor (β-AR) stimulation through sympathetic nervous system activation. Augmented glutaminolysis with increased bioavailability of α-ketoglutarate (α-KG) is suggested to contribute to fibrogenesis and changes in cellular gene expression. K3.

Efficient Separation and Enrichment of Rubidium in Salt Lake Brine Using High-Performance PAN-KCuFC-PEG Adsorption Composite.

Salt lake brine contains abundant rubidium resources; however, the separation of rubidium from brine with a high K content remains a significant challenge in metallurgical processes and materials science. In this study, PAN-KCuFC-PEG particles were synthesized by phase transformation, using hydrophilic polyacrylonitrile (PAN) as the skeleton structure, potassium cupric ferricyanide (KCuFC) as the active component and water-soluble polymer polyethylene glycol (PEG) as the pore regulator. Characterization revealed that the addition of PEG increased the pore volume of PAN-KCuFC-PEG by 63% and the BET surface area by 172%.

Hippo pathway activation mediates cardiomyocyte ferroptosis to promote dilated cardiomyopathy through downregulating NFS1.

Cardiomyocyte loss by regulated death modes, like apoptosis and ferroptosis, has been implicated in the development of dilated cardiomyopathy (DCM). It remains unclear whether cardiomyocyte ferroptosis occurs as a consequence of Hippo pathway activation. Using a mouse model of DCM by overexpression of Mst1 transgene (Mst1-TG) leading to Hippo pathway activation, we showed that cardiomyocyte ferroptosis was evident by transcriptomic profiles, elevated mitochondrial Fe content, increased levels of lipid peroxidation and obvious mitochondrial damage.

Mitochondrial Calcium Uniporter knockdown improves the viability of HT22 hippocampal neurons in Alzheimer's disease.

Alzheimer's disease (AD) is a degenerative disorder that poses a serious threat because it has no cure. Recently, mitochondrial dysfunction has been shown to directly promote the development of AD. An imbalance in mitochondrial calcium (mCa) homeostasis is an important cause of mitochondrial dysfunction.

Optimization and Clinical Application Potential of Single Nucleotide Polymorphism Detection Method Based on CRISPR/Cas12a and Recombinase Polymerase Amplification.

Conventional methods for detecting single nucleotide polymorphisms (SNPs) in clinical practice often require substantial time, labor, and specialized equipment, limiting their widespread application. To address this limitation, we refined our previous SNP detection method, IMAS-RPA [introducing an extra mismatched base adjacent to the single-base mutant site by recombinase polymerase amplification (RPA)], resulting in an updated version termed IMAS-RPAv2. We began by introducing a suboptimal protospacer adjacent motif (PAM) sequence, GTTG, into the double-stranded DNA (dsDNA) products using either RPA or reverse transcription RPA.

Epidemiology of cryptococcal meningitis and fluconazole heteroresistance in isolates from a teaching hospital in southwestern China.

Cryptococcal meningitis (CM), a common and serious opportunistic infection mostly caused by , is primarily treated with fluconazole. Nevertheless, strains that undergo repeated exposure to azoles can gradually acquire heteroresistance to fluconazole. The management of this specific CM infection poses a substantial challenge.

Green one-step synthesis of N-doped carbon quantum dots for fluorescent detection of lemon yellow in soft drinks.

A new fluorescent sensor for the determination of lemon yellow was developed based on nitrogen-doped carbon quantum dots (N-CQDs), which were prepared via a hydrothermal method with dried pomelo peel and L-tyrosine. The N-CQDs exhibited the blue fluorescence with a quantum yield of 28 %. The sensing principle of N-CQDs was quenched by lemon yellow via static quenching.