CXCR4-LASP1-G9a-SNAIL axis drives NEPC transdifferentiation via induction of EMT and downregulation of REST.

Phenotypic switching is an emerging driver of cancer treatment resistance, yet early signals regulating this process remain unclear. Here, using longitudinal single-cell RNA sequencing, we mapped differentiation trajectories in the LTL331 prostate adenocarcinoma patient-derived xenograft (PDX) model undergoing neuroendocrine prostate cancer (NEPC) transformation post castration. Our analyses identified a key differentiation node marked by epithelial-mesenchymal transition (EMT) and repressor element-1 silencing transcription factor (REST) downregulation driven by the CXCR4-LASP1-G9a-SNAIL axis.

Squamous cell carcinoma of unknown primary (SCCUP): a genomic landscape study.

Background: Squamous cell carcinoma (SCC) of unknown primary (SCCUP) refers to any SCC for which the primary tumor origin cannot be identified despite guideline-directed evaluation. Although strategies employing comprehensive genomic profiling (CGP) to identify targets for non-SCC carcinomas of unknown primary (CUPs) have shown benefit, the genomic landscape in SCCUP remains poorly defined. Here we describe results of CGP in patients with SCCUP to identify potential therapy targets and characteristic biomarkers.

Construction and evaluation of platelet concentrate/mesoporous bioactive glass composite scaffolds for bone repair: injectable platelet-rich fibrin (i-PRF) concentrated growth factors (CGF).

Rapid repair and functional reconstruction of maxillofacial bone defects is a significant challenge in the field of stomatology. Artificial bone substitute materials show promising potential in maxillofacial bone regeneration. This study investigated the bone regenerative effects of platelet concentrate/mesoporous bioactive glass (MBG) composite scaffolds, comparing injectable platelet-rich fibrin (i-PRF) and gel-phase concentrated growth factors (gpCGF).

Cost per responder for teclistamab and elranatamab in relapsed or refractory multiple myeloma in the United States.

Aims: Teclistamab and elranatamab are bispecific antibodies recently approved for the treatment of triple class-exposed relapsed/refractory multiple myeloma (RRMM). This study assessed the relative efficacy and economic value of teclistamab and elranatamab through a matching-adjusted indirect comparison (MAIC) and cost per responder analysis using data from the MajesTEC-1 (NCT03145181/NCT04557098) and MagnetisMM-3 (NCT04649359) trials.

Materials And Methods: The MAIC compared overall response rate (ORR) between the therapies after weighting individual patient data from MajesTEC-1 to match key baseline characteristics in MagnetisMM-3.

Enamel remineralization enhancement by mesoporous bioactive glass (MBG) in commercial fluoride varnish and development of an MBG/fluoride chitosan composite hydrogel.

With its excellent bioactivity and mesoporosity that endows controlled release of chemical or biological cues, mesoporous bioactive glass (MBG) is widely used in bone regeneration, yet less recognized in dentistry. This study investigates the enamel remineralization effect of MBG in commercial fluoride varnishes (FVs), and developes an MBG/fluoride chitosan composite hydrogel. MBG powders are added into commercial FVs (3M™ Clinpro™, Duraphat™) at different ratios to screen out the optimized proportion for mineralization.

Lipid-targeted echo-planar spectroscopic imaging technique for in vivo lipid composition measurement.

Purpose: Accurate in vivo assessment of lipid composition with high resolution and full abdominal coverage is essential for understanding lipid metabolism. This study aimed to develop and evaluate a novel lipid-targeted imaging technique, chemical shift-selective adiabatic refocusing spin-echo echo-planar spectroscopic imaging (EPSI) (Csar-EPSI), for rapid, precise, and reliable lipid composition measurements.

Methods: Csar-EPSI was designed with adiabatic pulses to selectively refocus lipid signals, suppress water, minimize chemical-shift displacement errors, and enable flexible voxel selection.

Patterns of intra- and intertumor phenotypic heterogeneity in lethal prostate cancer.

Metastatic prostate cancer (mPC) is a clinically and molecularly heterogeneous disease. While there is increasing recognition of diverse tumor phenotypes across patients, less is known about the molecular and phenotypic heterogeneity present within an individual. In this study, we aimed to define the patterns, extent, and consequences of inter- and intratumoral heterogeneity in lethal prostate cancer.

Airflow: A double-edged sword in shaping white tea quality.

Airflow significantly impacts white tea quality by regulating moisture loss and physiochemical reactions. LF-NMR, TEM, SEM, GC-TOF-MS, and UPLC-TQS-MS were used to compare the impacts of withering with different airflows, 0 m/s (AF0), 0.5 m/s (AF0.

Integrating ultrasound and serum indicators for evaluating outcomes of targeted immunotherapy in advanced liver cancer.

Background: Hepatocellular carcinoma (HCC) is a major global contributor to cancer-related mortality, with advanced stages presenting substantial therapeutic challenges. Although targeted immunotherapy shows potential, many patients exhibit poor responses, underscoring the need for predictive tools to optimize treatment strategies. Emerging data indicate that ultrasound features (, tumor stiffness) and serum biomarkers may serve as predictors of treatment outcomes.

Bioactive metallic nanoparticles for synergistic cancer immunotherapy.

Cancer immunotherapy has emerged as a promising strategy. However, low response rates and immune-related side effects have plagued immunotherapy. Metallic nanoparticles, utilizing metals as their framework, are gaining prominence in cancer immunotherapy.

Hiding in the kidney: a series of 13 lymphoid proliferations clinically mimicking renal carcinoma.

Masses are commonly detected in the kidneys either as incidental findings or upon workup of urinary symptoms on an initial presentation. We describe a large series of rare cases in which individuals presented with symptoms or imaging findings of kidney masses concerning for primary carcinoma of renal cell or urothelial origin but were found on pathological analysis to be lymphomas or atypical lymphoid proliferations, with or without an accompanying renal malignancy. These mass lesions, including some unique mimics of renal carcinomas such as reactive follicular hyperplasia and IgG4-related disease primary to the kidney, are all lymphoid kidney proliferations from a single institution.

Observation of the W-Annihilation Process D_{s}^{+}→ωρ^{+} and Measurement of D_{s}^{+}→ϕρ^{+} in D_{s}^{+}→π^{+}π^{+}π^{-}π^{0}π^{0} Decays.

We present the first amplitude analysis and branching fraction measurement of the decay D_{s}^{+}→π^{+}π^{+}π^{-}π^{0}π^{0}, using e^{+}e^{-} collision data collected with the BESIII detector at center-of-mass energies between 4.128 and 4.226 GeV corresponding to an integrated luminosity of 7.

Denoising Motion-Corrupted Seismocardiogram Signals Using Score-Based Generative Diffusion Models.

Noninvasive monitoring of hemodynamic parameters is essential for assessing cardiovascular function to enable early identification of high-risk individuals and help prevent injuries. However, current wearable solutions that use the electrocardiogram or photoplethysmogram offer a limited view of cardiovascular health, particularly in capturing cardiomechanical function. The seismocardiogram (SCG), a cardiomechanical signal, has shown promise in filling this gap with features previously shown to reflect key hemodynamic parameters.

3-Hydroxybutyrate Suppresses Colon Cancer Growth by Inducing Intracellular Lactate Accumulation and Oxidative Stress.

Cancer cachexia (CAC) remains a significant hurdle in the treatment of colon cancer, often resulting in poor clinical outcomes. This study explores the therapeutic potential of 3-hydroxybutyrate (3-HB) in the treatment of colon CAC by assessing its effects on tumor growth and cellular metabolism in a colon CAC mouse model and CT26 colon cancer cells. Using NMR-based metabolomics and molecular biology techniques, we show that 3-HB significantly suppresses tumor growth in CAC mice, possibly through lactate accumulation in tumor tissue and modulation of key metabolic pathways.

Mie-optimized PMMA particles for fully polymer-based radiative cooling coatings with high reflectance and hydrophobicity.

Passive radiative cooling materials achieve cooling without energy consumption by reflecting sunlight and emitting thermal radiation to the cold outer space (3 K). This study synthesizes poly(methyl methacrylate) (PMMA) particles with Mie scattering properties through emulsifier-free emulsion and dispersion polymerization to control particle size, enhancing their scattering and reflectivity. A high-emissivity polydimethylsiloxane (PDMS) matrix is used to fabricate a composite material, enabling complementary radiative effects between PMMA and PDMS within the atmospheric transparency window, thus enhancing the cooling efficiency.

Deep Blood Proteomics Identifies over 12,000 Proteins Providing Valuable Information about the State of the Human Body.

Blood is a valuable resource for clinical research, offering insight into physiological and pathological states. However, the specific proteins detectable in blood and the optimal proteomic methods for their detection have not been rigorously investigated and documented. To address this, we conducted various blood proteomic strategies, including directly blood proteomic analysis, high-abundance protein depletion, low-abundance protein enrichment, and extracellular vesicle enrichment using data-independent acquisition or targeted proteomics.

Perivascular matrix densification dysregulates angiogenesis and activates pro-inflammatory endothelial cells.

Fibrosis is central to numerous fatal conditions including solid cancers, pulmonary fibrosis, cirrhosis and post-infarct cardiac fibrosis amongst many others, thereby collectively contributing to 45% of all deaths in developed nations. The potential for fibrosis across most organ systems may stem from its connections to wound healing and the ubiquitous presence of vascular endothelium. Endothelial cells (ECs) and angiogenesis, cells and associated biological program central to wound healing, have been heavily implicated in many organ-specific fibroses, but the relationship between angiogenesis and fibrogenesis remains debated and little has been established in terms of how the EC phenotype governs tissue healing vs.

The effect of daily physical activity on bidirectional transitions of elevated blood pressure status: the first longitudinal evidence from the CHARLS.

Purpose Review: Physical activity (PA) is known to prevent and manage hypertension, but its impact on dynamic blood pressure (BP) transitions remains unclear. This study explores how daily PA influences bidirectional BP transitions using data from a Chinese national cohort.

Methods And Results: Using data from 2,785 CHARLS participants, BP states (normal, elevated, hypertensive) and PA intensity (vigorous, moderate, walking) were analyzed via multi-state Markov models.

Overlooked Trigger of Migraine: Clinical Significance of Small- and Medium-Shunt Patent Foramen Ovale.

Introduction: Patent foramen ovale (PFO) is a prevalent congenital heart malformation closely linked with migraine. The effect of PFO size on migraine remains controversial.

Methods: This study analyzed migraine patients who underwent PFO closure at our institution from January 2020 through December 2022.

Analysis of the factors influencing the dissemination of Mazu culture in Southeast Asia during the Qing Dynasty.

Mazu culture has had a profound impact on Southeast Asia. Its widespread dissemination during the Qing Dynasty resulted from the combined influence of various factors. This study uses the number of Mazu temples in Southeast Asia during the Qing Dynasty as a quantitative indicator and employs Pearson, Spearman, and Kendall correlation analyses to reveal the central role of geographical environment in cultural diffusion.

Burden of illness among patients with relapsed or refractory multiple myeloma, and eligible for B-cell maturation antigen-targeted therapies.

Background: B-cell maturation antigen (BCMA)-targeted therapies were approved for triple-class exposed relapsed/refractory multiple myeloma (RRMM) after ≥4 lines of therapy.

Methods: This claims-based retrospective analysis examined clinical and economic burden among United States patients who met eligibility for BCMA-targeted therapies and started a new therapy between 1 January 2016 and 30 September 2022.

Results: Of 228 eligible patients (median age: 64, 52.

Climate warming fuels the global antibiotic resistome by altering soil bacterial traits.

Understanding the implications of global warming on the spread of antibiotic resistance genes (ARGs) and virulence factor genes (VFGs) within soil ecosystems is crucial for safeguarding human well-being and sustaining ecosystem health. However, there is currently a lack of large-scale, systematic underpinning data needed to examine this issue. Here, using an integrative approach that combines field experiments, extensive global metagenomic data and microbial culturing, we show that warming enriches bacteria with ARGs and VFGs, increases metabolic complexity and adaptability in bacteria, and accelerates genetic alterations related to ARG and VFGs development.

Localization and characterization of cutaneous neurogenic inflammation in acute gastric mucosal injury in rats: A possible morphological explanation for visceral sensitization?

This investigation transcends traditional methodologies by providing a quantitative analysis of the dynamic relationship between visceral pathologies and neurogenic spots, employing an acute gastric mucosal injury (AGMI) rat model to map the somatotopic distribution of visceral sensitization. Through hydrochloric acid-induced plasma extravasation and Evans Blue dye (EB) marking, coupled with a geospatial grid system and multivariate statistical analysis, we identified Feature Regions (FRs) with distinct neurogenic responses. Notably, the right T10-13 dermatomere, or FR-11', exhibited elevated levels of nociceptive neuropeptides and serotonin, indicative of its significant role in pain perception.