State-of-the-art Neurointervention for Extended Window and Large Ischemic Core.

The implementation of endovascular therapy as a treatment for acute ischemic stroke has developed greatly in recent years. The primary trials demonstrated negative to mixed results, and it wasn't until the MR CLEAN trial that thrombectomy was established as a beneficial treatment. Since then, further studies have continued to provide additional data allowing for expansion of patient eligibility to receive such therapy.

Ethnic-Specific and UV-Independent Mutational Signatures of Basal Cell Carcinoma in Koreans.

Basal cell carcinoma (BCC), the most common skin cancer, is primarily driven by Hedgehog (Hh) and TP53 pathway alterations. Although additional pathways were implicated, the mutational landscape in Asian populations, particularly Koreans, remains underexplored. We performed whole-exome sequencing of BCC tumor tissues from Korean patients and analyzed mutations in 11 established BCC driver genes (, , , , , , , , and ).

Deep-Learning Model for Real-Time Prediction of Recurrence in Early-Stage Non-Small Cell Lung Cancer: A Multimodal Approach (RADAR CARE Study).

Purpose: The surveillance protocol for early-stage non-small cell lung cancer (NSCLC) is not contingent upon individualized risk factors for recurrence. This study aimed to use comprehensive data from clinical practice to develop a deep-learning model for practical longitudinal monitoring.

Methods: A multimodal deep-learning model with transformers was developed for real-time recurrence prediction using baseline clinical, pathological, and molecular data with longitudinal laboratory and radiologic data collected during surveillance.

Cerebral Tissue Oximetry Monitoring for Severe Brain Injury.

Neurocritical care management for severe brain injuries (SBI) has traditionally focused on intracranial pressure (ICP) and cerebral perfusion pressure (CPP) monitoring and treatment to prevent brain herniation and ischemia. However, the complex pathophysiology of secondary brain injury in SBI patients involves various mechanisms, including oxygen metabolism and transport disturbances, mitochondrial dysfunction, and signaling cascade disruption. Multimodal neuromonitoring (MMN) techniques and modern high-technology resources are being utilized to detect these detrimental phenomena and guide individualized clinical management strategies.

Development and validation of a transformer model-based early warning score for real-time prediction of adverse outcomes in the emergency department.

This study aimed to develop and validate a transformer-based early warning score (TEWS) system for predicting adverse events (AEs) in the emergency department (ED). We conducted a retrospective study analyzing adult ED visits at a tertiary hospital. The TEWS was developed to predict five AEs within 24 h: vasopressor use, respiratory support, intensive care unit admission, septic shock, and cardiac arrest.

BOOTStrap-SCI: Beyond One Option of Treatment for Spinal Trauma and Spinal Cord Injury: Consensus-Based Stratified Protocols for Intensive Care and Surgical Management.

Objective: Spinal trauma (STx), with or without spinal cord injury (SCI), represents a significant global health burden, particularly in low- and middle-income countries (LMICs). International guidelines often assume the availability of resources for intensive care and surgical management, limiting their applicability in resource-constrained settings. A resource-adapted approach may improve management in these contexts.

ASOptimizer: optimizing chemical diversity of antisense oligonucleotides through deep learning.

Antisense oligonucleotides (ASOs) are a promising class of gene therapies that can modulate the gene expression. However, designing ASOs manually is resource-intensive and time-consuming. To address this, we introduce a user-friendly web server for ASOptimizer, a deep learning-based computational framework for optimizing ASO sequences and chemical modifications.

Assessing the Effect of Automated Thermo-Mechanical Therapy on Abdominal Blood Flow With Magnetic Resonance Imaging.

Thermal and mechanical tissue stimulation is widely utilized in various medical contexts, particularly to enhance local circulation, alleviate pain, and restore movement. Techniques to objectively quantify the physiological effects of these interventions support therapeutic efficacy and explain clinical benefits. Here we conducted a pilot trial to evaluate the feasibility of magnetic resonance imaging (MRI) technology to provide an objective assessment of acute treatment effects in enhancing blood flow.

Evolutionary Insights into Irisin/FNDC5: Roles in Aging and Disease from to Mammals.

The Irisin/FNDC5 protein family has emerged as a pivotal link between exercise and the prevention of age-associated diseases. Irisin is highly expressed during exercise from skeletal and cardiac muscle cells, playing a critical role in mediating systemic health benefits through its actions on various tissues. However, Irisin levels decline with age, correlating with a heightened incidence of diseases such as muscle weakness, cardiovascular disorders, and neurodegeneration.

Transcription factor RUNX1 regulates coagulation factor XIII-A (): decreased platelet-megakaryocyte expression and clot contraction in haplodeficiency.

Background: Germline haplodeficiency (RHD) is associated with thrombocytopenia, platelet dysfunction, and predisposition to myeloid malignancies. Platelet expression profiling of an RHD patient showed decreased , encoding for the A subunit of factor (F)XIII a transglutaminase that cross-links fibrin and induces clot stabilization. FXIII-A is synthesized by hematopoietic cells, megakaryocytes, and monocytes.

Diagnosis of Chronic Kidney Disease Using Retinal Imaging and Urine Dipstick Data: Multimodal Deep Learning Approach.

Background: Chronic kidney disease (CKD) is a prevalent condition with significant global health implications. Early detection and management are critical to prevent disease progression and complications. Deep learning (DL) models using retinal images have emerged as potential noninvasive screening tools for CKD, though their performance may be limited, especially in identifying individuals with proteinuria and in specific subgroups.

Takotsubo Cardiomyopathy After Subarachnoid Hemorrhage: Who Is At Risk?

IntroductionTakotsubo Cardiomyopathy (TCM) is a rare but well recognized complication of subarachnoid hemorrhage associated with increased morbidity and poor clinical outcomes. There is a scarcity of literature describing the prevalence and risk factors associated with this complication. The aim of this study was to identify patients who are at risk of developing TCM in non-traumatic subarachnoid hemorrhage.

Light Quality Plays a Crucial Role in Regulating Germination, Photosynthetic Efficiency, Plant Development, Reactive Oxygen Species Production, Antioxidant Enzyme Activity, and Nutrient Acquisition in Alfalfa.

Light is a vital regulator of photosynthesis, energy production, plant growth, and morphogenesis. Although these key physiological processes are well understood, the effects of light quality on the pigment content, oxidative stress, reactive oxygen species (ROS) production, antioxidant defense systems, and biomass yield of plants remain largely unexplored. In this study, we applied different light-emitting diode (LED) treatments, including white light, red light, blue light, and a red+blue (1:1) light combination, to evaluate the traits mentioned above in alfalfa ( L.

Transcription Factor RUNX1 Regulates Coagulation Factor XIII-A ( ): Decreased Platelet-Megakaryocyte Expression and Clot Contraction in Haplodeficiency.

Background: Germline haplodeficiency (RHD) is associated with thrombocytopenia, platelet dysfunction and predisposition to myeloid malignancies. Platelet expression profiling of a RHD patient showed decreased encoding for the A subunit of factor XIII, a transglutaminase that cross-links fibrin and induces clot stabilization. FXIII-A is synthesized by hematopoietic cells, megakaryocytes and monocytes.

Changes in microbial dynamics and fermentation characteristics of alfalfa silage: A potent approach to mitigate greenhouse gas emission through high-quality forage silage.

Feeding ruminants with high-quality forage can enhance digestibility and reduce methane production. Development of high-quality silage from leguminous plants with lactic acid bacteria can improve digestibility and it mitigate the greenhouse gas emissions. In this study, we developed a high-quality alfalfa silage with improved fermentation index and microbial dynamics using Levilactobacillus brevis-KCC-44 at low or high moisture (LM/HM) conditions and preserved it for 75 or 150 days.

Studies of infused megakaryocytes into mice support a "catch-and-release" model of pulmonary-centric thrombopoiesis.

Unlabelled: Many aspects of thrombopoiesis, the release of platelets from megakaryocytes (Mks), remain under debate, including where this process occurs. Murine lung -microscopy studies suggested that a significant fraction of circulating platelets were released from lung-entrapped, marrow-derived Mks. We now confirm these studies that endogenous mMks are entrapped in the lungs and show that intravenously infused -differentiated, mature murine (m) and human (h) Mks are similarly entrapped followed by shedding of their cytoplasm over ∼30 minutes with a peak number of released platelets occurring 1.

ASOptimizer: Optimizing antisense oligonucleotides through deep learning for IDO1 gene regulation.

Recent studies have highlighted the effectiveness of using antisense oligonucleotides (ASOs) for cellular RNA regulation, including targets that are considered undruggable; however, manually designing optimal ASO sequences can be labor intensive and time consuming, which potentially limits their broader application. To address this challenge, we introduce a platform, the ASOptimizer, a deep-learning-based framework that efficiently designs ASOs at a low cost. This platform not only selects the most efficient mRNA target sites but also optimizes the chemical modifications for enhanced performance.

Brief Report: Self-Reported HIV-Positive Status but Subsequent HIV-Negative Test Results in Population-Based HIV Impact Assessment Survey Participants-11 Sub-Saharan African Countries, 2015-2018.

Background: HIV testing is a critical step to accessing antiretroviral therapy (ART) because early diagnosis can facilitate earlier initiation of ART. This study presents aggregated data of individuals who self-reported being HIV-positive but subsequently tested HIV-negative during nationally representative Population-Based HIV Impact Assessment surveys conducted in 11 countries from 2015 to 2018.

Method: Survey participants aged 15 years or older were interviewed by trained personnel using a standard questionnaire to determine HIV testing history and self-reported HIV status.

Altered platelet-megakaryocyte endocytosis and trafficking of albumin and fibrinogen in RUNX1 haplodeficiency.

Platelet α-granules have numerous proteins, some synthesized by megakaryocytes (MK) and others not synthesized but incorporated by endocytosis, an incompletely understood process in platelets/MK. Germ line RUNX1 haplodeficiency, referred to as familial platelet defect with predisposition to myeloid malignancies (FPDMMs), is associated with thrombocytopenia, platelet dysfunction, and granule deficiencies. In previous studies, we found that platelet albumin, fibrinogen, and immunoglobulin G (IgG) were decreased in a patient with FPDMM.

Corrigendum: Use of electroencephalogram, gait, and their combined signals for classifying cognitive impairment and normal cognition.

[This corrects the article DOI: 10.3389/fnagi.2022.

Association of plasma amyloid-β oligomerization with theta/beta ratio in older adults.

Background: Oligomeric Aβ (OAβ) is a promising candidate marker for Alzheimer's disease (AD) diagnosis. Electroencephalography (EEG) is a potential tool for early detection of AD. Still, whether EEG power ratios, particularly the theta/alpha ratio (TAR) and theta/beta ratio (TBR), reflect Aβ burden-a primary mechanism underlying cognitive impairment and AD.

Altered Platelet-Megakaryocyte Endocytosis and Trafficking of Albumin and Fibrinogen in Haplodeficiency.

Unlabelled: Platelet α-granules have numerous proteins, some synthesized by megakaryocytes (MK) and others not synthesized but incorporated by endocytosis, an incompletely understood process in platelets/MK. Germline haplodeficiency, referred to as familial platelet defect with predisposition to myeloid malignancies (FPDMM), is associated with thrombocytopenia, platelet dysfunction and granule deficiencies. In previous studies, we found that platelet albumin, fibrinogen and IgG levels were decreased in a FPDMM patient.

Correlation between electrodiagnostic severity and Boston carpal tunnel questionnaire in surgically treated carpal tunnel syndrome patients.

Objective: The objectives of this study were to (1) investigate whether electrodiagnostic (EDX) findings reflect the preoperative symptom severity and functional impairment in patients with carpal tunnel syndrome (CTS) and (2) evaluate the relationship between EDX findings and the clinical outcomes reported according to the self-administered patient questionnaires.

Methods: Patients diagnosed with idiopathic CTS who underwent carpal tunnel release between May 2016 and July 2018 were included. Carpal tunnel syndrome was clinically diagnosed and confirmed based on the EDX findings.