Auramine O, food dye induces cognitive impairment, anxiety, alters synaptic transmission and neural growth in zebrafish model.

Synthetic food dyes, widely used to enhance the visual appeal of food and beverages, are associated with potential neurobehavioral impacts, particularly in children. Despite its banned status in many regions, Auramine O (AO) has been detected as a contaminant in various food products. In silico studies have demonstrated that AO can induce neurotoxic effects.

Regulation of oxytocin receptor by zinc coumarin derivatives: a mechanistic approach to alleviate anxiety and enhance folliculogenesis in letrozole-induced PCOS in zebrafish model.

Polycystic ovary syndrome (PCOS) is a common endocrine disorder in women, characterized by insulin resistance and mood disturbances. The therapeutic potential of traditional treatments is often limited by side effects, highlighting the need for novel interventions. This study investigated the efficacy of newly synthesized thiosemicarbazone coumarin zinc complexes, TSCO6-Zn (T1) and TSCO13-Zn (T2) were assessed in a letrozole-induced PCOS in-vivo zebrafish model.

Association of CYP27B1 promoter gene variants of vitamin D pathway with pulmonary tuberculosis and vitamin D levels.

Cyp27b1 polymorphisms are stated to be associated with different diseases including tuberculosis (TB). Since the gene variants located in the promoter region may have a significant influence on gene transcription/translation and Cyp27b1 enzyme is involved in critical steps in vitamin D metabolism, we aim to study whether Cyp27b1 gene promoter variants namely -1077 (C/G), -1260 (C/A) and the region immediately 5' to the promoter -1918 (C/T) have any linkage with pulmonary tuberculosis risk/defence and to determine their influence on vitamin D level in normal healthy controls (HCs) and pulmonary tuberculosis (PTB) patients of the South Indian population. The polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) method were used to genotype the genomic DNA after it was extracted using the salting-out approach.

Renal protective efficiency of Isatin-linked Pyrazole (3E) derivative which mitigates gentamicin-induced nephrotoxicity and its anti-fibrotic mechanisms in an in-vivo zebrafish model.

Gentamicin (Gen), a commonly used aminoglycoside antibiotic, is associated with significant nephrotoxicity driven by oxidative stress, inflammation, and fibrosis, leading to renal impairment. This study investigates the therapeutic potential of the Isatin-linked Pyrazole (3E) derivative (or compound) in mitigating Gen-induced nephrotoxicity using in-vivo zebrafish as a model organism. The anti-inflammatory, antioxidant, and anti-fibrotic properties of the 3E derivative were assessed through biochemical, molecular, and histopathological analyses.

Effectiveness of 6-nitrobenzo[d]thiazol-2-amine derivative (N2) in ameliorating ethanol-induced fatty liver through lipogenesis inhibition and anti-inflammatory expression in a zebrafish model.

This study examines the effectiveness of the 6-nitrobenzo[d]thiazol-2-amine derivative (N2) in mitigating the effects of ethanol-induced fatty liver disease in zebrafish by assessing its anti-inflammatory, antioxidant, and antihyperlipidemic properties. The investigation encompasses the evaluation of N2 derivatives on hemolysis, cytotoxicity, developmental toxicity, oxidative stress, and lipid metabolism within zebrafish models. Antioxidant assays highlighted N2's significant radical scavenging activity.

Efficacy of 6-nitrobenzo[d]thiazol-2 Amine Derivative (N3) in Mitigating PTZ-Induced Epileptic Conditions Via Modulation of Inflammatory and Neuroprotective Pathways in-vivo Zebrafish.

Epilepsy, a chronic neurological illness affecting 50 million people worldwide, causes recurring seizures due to abnormal brain activity. Current anti-epileptic medications have serious side effects and low efficacy, requiring alternative treatments. The current study investigated the anti-inflammatory, antioxidant, and neuroprotective effects of 6-nitrobenzo[d]thiazol-2-amine (N3) derivatives in a zebrafish larvae model of epilepsy caused by 6 mM pentylenetetrazole (PTZ).

Development and evaluation of chitosan conjugated Isatin-linked Pyrazole derivative to target anti-inflammatory and anti-fibrotic in ethanol induced liver fibrosis using an in-vivo zebra fish model.

Alcoholic liver disease (ALD) remains a health burden, characterized by hepatic steatosis to fibrosis, a significant contributor to global morbidity and mortality, with limited therapeutic options for advanced stages like liver fibrosis. This study explores the antifibrotic and anti-inflammatory potential of a novel isatin-linked pyrazole derivative (3F) conjugated with chitosan-EDTA (CS) in mitigating ethanol (EtOH) induced liver fibrosis in zebrafish model. We demonstrated hepatic fibrosis using a chronic low-dose EtOH model (0.

SG06, a Chalcone Derivative Targets PI3K/AKT1 Pathway for Neuroprotection and Cognitive Enhancement in an Alzheimer's Disease-Like Zebrafish Model.

Alzheimer's disease (AD) and Alzheimer's dementia (ADM) are common neurodegenerative disorders marked by progressive cognitive decline, memory impairment, and behavioral deficits, which impose a significant burden on individuals and healthcare systems worldwide. Due to the complex nature of AD pathophysiology, effective treatment strategies may require targeting multiple pathways. This study explored the neuroprotective effects of the chalcone derivative SG06 in a scopolamine-induced AD-like zebrafish model using network pharmacology and molecular docking.

Isatin-linked pyrazole K1 derivative alter the phosphatidylinositol-3-kinase pathway by enhancing the metabolic function and folliculogenesis in the triclosan-induced PCOS-like condition in zebrafish model.

Polycystic ovarian syndrome (PCOS), which causes hormonal imbalance, inflammation, and metabolic disorders, requires several treatments. This study aimed to examine the Isatin-linked pyrazole K1 derivative's effectiveness in PCOS induced by environmental contaminants such as triclosan, specifically assessing its biochemical, metabolic, and reproductive impacts. Isatin-linked pyrazole K1 derivative was synthesised in the lab and tested in vitro and in vivo, including cytotoxicity testing in CHO cells, apoptosis analysis in AO/PI staining, and developmental toxicity in zebrafish embryos.

Exploring the potential of bacterial-derived EVs for targeted enzyme replacement therapy: mechanisms, applications, and future directions.

Extracellular vesicles (EVs) are membrane-bound vesicles produced by cells which promote intercellular communication by delivering different contents such as DNA, RNA, and proteins. These vesicles, nano-sized and released into the extracellular space, are present everywhere under both normal and pathological conditions. Probiotic-derived EVs can serve as nanocarriers for therapeutic cargo, particularly in enzyme replacement therapy (ERT).

Black seed (Nigella sativa) extract enhances early and late apoptosis through activation of caspase-3 mediated regulatory pathway in LC540 cells: A network pharmacological and molecular docking approach.

Testicular cancer continues to rise in incidence globally, and conventional chemotherapy is often associated with severe side effects that significantly impact patient's quality of life. Identifying safer alternative therapies is becoming crucial day by day which leads to focus on naturally available phytocompounds with high bioactivity. Over the period of time, Nigella sativa (N.

Position: Topological Deep Learning is the New Frontier for Relational Learning.

Topological deep learning (TDL) is a rapidly evolving field that uses topological features to understand and design deep learning models. This paper posits that TDL is the new frontier for relational learning. TDL may complement graph representation learning and geometric deep learning by incorporating topological concepts, and can thus provide a natural choice for various machine learning settings.

Exposure to bisphenol A and sodium nitrate found in processed meat induces endocrine disruption and dyslipidemia through PI3K/AKT/SREBP pathway in zebrafish larvae.

Meat is a staple in many cultural diets, and the consumption of processed meats has increased significantly worldwide. The widespread use of sodium nitrate (NaNO) as a preservative and the unintentional leaching of bisphenol A (BPA) from packaging into meats have raised health concerns. This study evaluates the combined toxicity of BPA and NaNO despite their individual safety assessments.

Liquid mosquito repellent induces toxic effects in zebrafish.

Mosquitoes serve as vectors for life-threatening parasitic diseases, presenting a continuous threat throughout human history. This has resulted in the extensive utilization of various mosquito repellents, including liquid mosquito repellents (LMRs), roll-ons, and topical creams. While these products demonstrate significant efficacy, the toxicological implications associated with their use are not yet fully understood and continue to be a subject of debate.

A combinational threat of micro- and nano-plastics (MNPs) as potential emerging vectors for per- and polyfluoroalkyl substances (PFAS) to human health.

Micro- and nano-plastics (MNPs) and per- and polyfluoroalkyl substances (PFAS) are prevalent in ecosystems due to their exceptional properties and widespread use, profoundly affecting both human health and ecosystem. Upon entering the environment, MNPs and PFAS undergo various transformations, such as weathering, transport, and accumulation, potentially altering their characteristics and structural dynamics. Their interactions, governed by factors like hydrogen bonding, hydrophobic interactions, Van der Waals forces, electrostatic attractions, and environmental conditions, can amplify or mitigate their toxicity toward human health within ecological conditions.

Protective role of 2-aminothiazole derivative against ethanol-induced teratogenic effects in-vivo zebrafish.

Teratology investigates the origins of congenital disabilities, often linked to environmental factors such as ethanol (EtOH) exposure. Ethanol at 150 μM has been associated with teratogenic effects, oxidative stress, immunological responses, and endocrine disruptions. Fetal alcohol spectrum disorder (FASD) arises from maternal alcohol consumption during pregnancy, leading to developmental delays and cognitive impairment.

Neurotoxic and developmental effects of scented incense stick smoke: Network toxicology and zebrafish model study.

Burning incense sticks is a traditional practice in many cultures, especially in Southeast Asia. While it is often regarded as sacred and beneficial, modern incense sticks contain various chemicals that can pose health risks. A GCMS analysis of the ICS revealed potential compounds.

Combined effects of vitamin D3 and dioxopiperidinamide derivative on lipid homeostasis, inflammatory pathways, and redox imbalance in non-alcoholic fatty liver disease in vivo zebrafish model.

Liver damage and metabolic dysfunctions, the defining features of non-alcoholic fatty liver disease (NAFLD), are marked by inflammation, oxidative stress, and excessive hepatic fat accumulation. The current therapeutic approaches for NAFLD are limited, necessitating exploring novel treatment strategies. Dioxopiperidinamide derivatives, particularly DOPA-33, have shown effective anti-inflammatory and antioxidant properties, potentially offering therapeutic benefits against NAFLD.

Is Laccase derived from Pleurotus ostreatus effective in microplastic degradation? A critical review of current progress, challenges, and future prospects.

Exploration of Pleurotus ostreatus as a biological agent in the degradation of persistent plastics like polyethylene, polystyrene, polyvinyl chloride, and polyethylene terephthalate, revealing a promising avenue toward mitigating the environmental impacts of plastic pollution. Leveraging the intrinsic enzymatic capabilities of this fungus, mainly its production of laccase, presents a sustainable and eco-friendly approach to breaking down complex polymer chains into less harmful constituents. This review focused on enhancements in the strain's efficiency through genetic engineering, optimized culture conditions, and enzyme immobilization to underscore the potential for scalability and practical application of this bioremediation process.

Textile azo dye, Sudan Black B, inducing hepatotoxicity demonstrated in in vivo zebrafish larval model.

Environmental pollution, particularly from textile industry effluents, raises concerns globally. The aim of this study is to investigate the hepatotoxicity of Sudan Black B (SBB), a commonly used textile azo dye, on embryonic zebrafish. SBB exposure led to concentration-dependent mortality, reaching 100% at 0.

Cadmium and ketoprofen accumulation influences aquatic ecosystem demonstrated using zebrafish model.

The growing concern about pollution and toxicity in aquatic as well as terrestrial organisms is predominantly caused due to waterborne exposure and poses a risk to environmental systems and human health. This study addresses the co-toxic effects of cadmium (Cd) and ketoprofen (KPF), representing heavy metal and pharmaceutical discharge pollutants, respectively, in aquatic ecosystems. A 96-h acute toxicity assessment was conducted using zebrafish embryos.

Hues of risk: investigating genotoxicity and environmental impacts of azo textile dyes.

The textile industry, with its extensive use of dyes and chemicals, stands out as a significant source of water pollution. Exposure to certain textile dyes, such as azo dyes and their breakdown products like aromatic amines, has been associated with health concerns like skin sensitization, allergic reactions, and even cancer in humans. Annually, the worldwide production of synthetic dyes approximates 7 × 10 tons, of which the textile industry accounts for over 10,000 tons.

PI-Net: A Deep Learning Approach to Extract Topological Persistence Images.

Topological features such as persistence diagrams and their functional approximations like persistence images (PIs) have been showing substantial promise for machine learning and computer vision applications. This is greatly attributed to the robustness topological representations provide against different types of physical nuisance variables seen in real-world data, such as view-point, illumination, and more. However, key bottlenecks to their large scale adoption are computational expenditure and difficulty incorporating them in a differentiable architecture.

Learning Stable Multilevel Dictionaries for Sparse Representations.

Sparse representations using learned dictionaries are being increasingly used with success in several data processing and machine learning applications. The increasing need for learning sparse models in large-scale applications motivates the development of efficient, robust, and provably good dictionary learning algorithms. Algorithmic stability and generalizability are desirable characteristics for dictionary learning algorithms that aim to build global dictionaries, which can efficiently model any test data similar to the training samples.

Multiple kernel sparse representations for supervised and unsupervised learning.

In complex visual recognition tasks, it is typical to adopt multiple descriptors, which describe different aspects of the images, for obtaining an improved recognition performance. Descriptors that have diverse forms can be fused into a unified feature space in a principled manner using kernel methods. Sparse models that generalize well to the test data can be learned in the unified kernel space, and appropriate constraints can be incorporated for application in supervised and unsupervised learning.