Identification of Two Critical Contact Residues in a Pathogenic Epitope from Tetranectin for Monoclonal Antibody Binding and Preparation of Single-Chain Variable Fragments.

Sepsis is a fetal disease that requires a clear diagnostic biomarker for timely antibiotic treatment. Recent research has identified a pyroptosis-inducing epitope known as P5-5 in tetranectin (TN), a plasma protein produced by monocytes. Previously, we produced a 12F1 monoclonal antibody against the P5-5 and discovered that it could not only diagnose the presence but also monitor the progress of sepsis in the clinic.

Deciphering the complete mitochondrial genome of Halogeton glomeratus structural features and RNA editing events.

Halogeton glomeratus, a halophytic species in the Amaranthaceae family, is well adapted to extreme saline-alkaline environments. To better understand its adaptive mechanisms at the genomic level, we assembled and analyzed the complete mitochondrial genome (mitogenome) of H. glomeratus.

Transcriptome Analysis and Functional Characterization of the Gene Involved in Barley Resistance to .

Barley leaf stripe, caused by (), significantly reduces yields across various regions globally. Understanding the resistance mechanisms of barley to is crucial for advancing disease resistance breeding efforts. In this study, two barley genotypes-highly susceptible Alexis and immune Ganpi2-were inoculated with the highly pathogenic isolate QWC for 7, 14, and 18 days.

Endothelial plasticity in atrial fibrosis by integrating single-cell sequencing and genetic lineage tracing.

Background And Aims: Atrial fibrosis represents a critical determinant in atrial fibrillation (AF) pathogenesis. Although endothelial dysfunction is a hallmark feature of AF, the precise mechanisms by which endothelial cells (ECs) contribute to atrial fibrosis remain incompletely understood.

Methods: This study employed single-cell RNA sequencing (scRNA-seq) to analyze intercellular communication networks in atrial tissues from both sinus rhythm (SR) and AF patients.

Targeting PERP promotes anti-tumor immunity in HNSCC by regulating tumor immune microenvironment and metabolic homeostasis.

Background: PERP may have the potential to function as an oncogene. However, the precise function, prognostic value, and predictive significance remain shrouded in ambiguity.

Methods: We conducted an in-depth analysis using pan-cancer RNA sequencing data and various online web tools to investigate the correlation between PERP and crucial clinical outcomes such as prognosis, tumor microenvironment, and tumor metabolism.

Engineering overexpressing SYNGR1 inhibited the progression of GBM cells by suppressing the intracellular FGF1-mediated LDs accumulation and cytoskeleton remodeling.

Background: Glioblastoma (GBM) is the most common and aggressive glioma subtype in adults, characterized by disrupted lipid homeostasis. The accumulation of lipid droplets (LDs) is involved in the actin cytoskeleton remodeling in different types of cells, the intracellular fibroblast growth factor 1 (FGF1) plays an important role in this process. The stability of cytoskeleton is crucial for the proliferation, invasion and other malignant behavior of GBM.

Efflux of N1-acetylspermidine from hepatoma fosters macrophage-mediated immune suppression to dampen immunotherapeutic efficacy.

Metabolic reprogramming is a hallmark of tumor progression. Here, we examined the metabolic profile of hepatocellular carcinoma (HCC), a disease that responds poorly to immune checkpoint blockade (ICB). Polyamine metabolism increased in HCC samples.

The effect of clonal hematopoiesis on long-term outcomes in patients undergoing coronary artery bypass grafting.

Background: Clonal hematopoiesis of indeterminate potential (CHIP), characterized by age-related somatic mutations in hematopoietic stem and progenitor cells, has been identified as a potential risk factor for cardiovascular events and mortality. However, the significance of CHIP in the context of coronary artery bypass grafting (CABG) remains unexplored. We aim to investigate the potential impact of CHIP on long-term outcomes of patients who underwent CABG.

Peripheral eosinophils and immunotherapy response in patients with recurrent or metastatic HNSCC.

This study investigates the relationship between absolute eosinophil count (AEC) and eosinophil percentage (EOS%) with response rates to immune checkpoint inhibitors (ICIs) in recurrent or metastatic head and neck squamous cell carcinoma (R/M HNSCC) patients. The analysis included data from R/M HNSCC patients treated with ICIs at Second Xiangya Hospital (2016-2021), assessing baseline characteristics, tumor specifics, immune-related adverse events, AEC, and EOS%. The correlation between these factors and ICI response rates was evaluated using logistic regression, while survival outcomes were analyzed through Kaplan-Meier curves and Cox models.

Interface Engineering by Small Molecules toward Efficient Hole Transport Layer-Free Sn-Pb Perovskite Solar Cells with High Fill Factors.

Low-bandgap (LBG) tin-lead (Sn-Pb) perovskites are essential for tandem solar cells but face challenges because the use of a hygroscopic PEDOT:PSS hole transport layer (HTL) reduces device stability. A HTL-free structure can overcome these issues but introduces new drawbacks like imbalanced carrier transport and severe recombination. This study introduces 3-amino-5-mercapto-1,2,4-triazole (AMTZ) as a rear interface passivator to simultaneously mitigate defects and stabilize Sn-Pb perovskite films.

Enhancing Diagnosis of Psoriasis and Inflammatory Skin Diseases: A Spatially Aligned Multimodal Model Integrating Clinical and Dermoscopic Images.

Psoriasis is a chronic inflammatory disease with significant physical and psychological impacts. To overcome the limitations of single-modality artificial intelligence models in diagnosing inflammatory skin diseases, we propose a multimodal framework-spatial alignment multimodal contrastive learning-integrating dermoscopic and clinical photographs and trained on the developed Peking Union Medical College Hospital - Inflammatory Skin Diseases dataset, which includes 8 inflammatory skin diseases. On the Peking Union Medical College Hospital - Inflammatory Skin Diseases dataset, our model achieved classification accuracies of 0.

Characterizing the wheat ( L.) phosphate transporter gene family and analyzing expression patterns in response to low phosphorus stress during the seedling stage.

Introduction: Inorganic phosphorus (Pi) is an indispensable nutrient for plant growth, with phosphate transporter proteins (PHTs) having key roles in Pi uptake, transport, and signal transduction in plants. However, a systematic and comprehensive genomic analysis of the wheat family (covering and ) is lacking.

Methods: In view of this, we successfully identified 180 () members in 6 PHT families using bioinformatics, and performed in-depth phylogenetic analyses between these protein sequences and family proteins from and an important rice crop.

Customized Multifunctional Additive Regulates 1.67 eV-Wide-Bandgap Perovskite Crystallization for Four-Terminal Perovskite/Silicon Tandem Solar Cells.

Wide-bandgap (WBG) perovskite solar cells (PSCs, E > 1.6 eV), serving as the top cell in perovskite/silicon tandem solar cells (PSTSCs), play an indispensable role in absorbing high energy photons and increasing overall efficiency. However, WBG PSCs often suffer from severe light-induced phase segregation and significant non-radiative recombination losses due to uncontrolled rapid crystallization.

The Essentials of , a Polygalacturonase-Encoding Gene for the Invasion of to .

Barley leaf stripe, caused by , significantly reduces yield. Polygalacturonase, a key fungal pectinase, facilitates cell wall degradation for nutrition acquisition and colonization. To determine whether contains polygalacturonase ()-encoding genes and their role in pathogenicity, four genes (-) were identified in the genome.

Numerical Modeling of Multiphysics Constitutive Relations Governing Water Migration into Variably Saturated Sorptive Shale.

The water invasion within shales easily causes damage to the hydrocarbon flow. However, the water imbibition mechanisms are still unclear, and the mathematical connection between stress-dependent permeability and water imbibition was rarely known. As a result, in this paper, a numerical simulation model coupled with water imbibition, shale deformation, and shale permeability alteration was established to analyze how water imbibition lead to an alteration in geomechanical properties and shale gas permeability.

Metagenomic analysis reveal the phytoremediation effects of monocropping and intercropping of halophytes Halogeton glomeratus and Suaeda glauca in saline soil of Northwestern China.

Aims: Planting halophytes is a widely used method of phytoremediation for saline soils. The succulent halophytes Halogeton glomeratus and Suaeda glauca are widely used for remediation of saline soil in the arid region of Northwestern China. However, whether intercropping of H.

Genetic Basis of Seedling Root Traits in Common Wheat ( L.) Identified by Genome-Wide Linkage Mapping.

Common wheat production is significantly influenced by abiotic stresses. Identifying the genetic loci for seedling root traits and developing the available molecular markers are crucial for breeding high yielding and stable varieties. In this study, five wheat seedling root traits, including root length (RL), root surface area (RA), root volume (RV), number of root tips (RT), and root dry weight (RW), were measured in the Wp-072/Wp-119 recombinant inbred line (RIL) population.

Transarterial Chemoembolization with Radiofrequency Ablation versus Surgical Resection for Small Late-Recurrence Hepatocellular Carcinoma.

Background Radiofrequency ablation (RFA) has comparable clinical outcomes to surgical resection (SR) for treating small recurrent hepatocellular carcinoma (HCC). However, whether combined transarterial chemoembolization (TACE) with RFA (hereafter, TACE-RFA) outperforms SR for treating small late-recurrence HCCs remains unknown. Purpose To compare the clinical outcome of TACE-RFA with that of SR in patients with small late-recurrence HCCs.

Hierarchical braking accurate control of electrohydraulic composite braking system for electric vehicles.

For the electrohydraulic composite braking system, the general total braking force calculation strategy frequently ignores the resist forces, thereby cannot track the braking intention of driver perfectly. Moreover, the torque allocation process reduces the control reliability and energy recovery effect. In this research, a novel hierarchical braking accurate control (HBAC) algorithm is designed to achieve both the control accuracy and the ideal energy recovery efficiency.

Biomaterial-assisted organoid technology for disease modeling and drug screening.

Developing disease models and screening for effective drugs are key areas of modern medical research. Traditional methodologies frequently fall short in precisely replicating the intricate architecture of bodily tissues and organs. Nevertheless, recent advancements in biomaterial-assisted organoid technology have ushered in a paradigm shift in biomedical research.

Dual targeting PPARα and NPC1L1 metabolic vulnerabilities blocks tumorigenesis.

Dysregulated lipid metabolism is linked to tumor progression. In this study, we identified Niemann-Pick C1-like 1 (NPC1L1) as a downstream effector of PKM2. In breast cancer cells, PKM2 knockout (KO) enhanced NPC1L1 expression while downregulating peroxisome proliferator-activated receptor α (PPARα) signaling pathway.

Comparing PD-L1 and PD-1 inhibitors plus bevacizumab combined with hepatic arterial interventional therapies in unresetable hepatocellular carcinoma: A single-center, real-world study.

With the rise of anti-vascular endothelial growth factor antibody and programmed cell death-ligand 1 (PD-L1) regimens, particularly bevacizumab and atezolizumab, as first-line treatments for advanced hepatocellular carcinoma (HCC), there is a need to explore PD-L1 and programmed cell death 1 inhibitors in combination therapies for unresectable HCC (uHCC). Integrating systemic therapies with locoregional approaches is also emerging as a potent strategy. This study compares the outcomes of atezolizumab (PD-L1 inhibitor) and sintilimab (programmed cell death 1 inhibitor) with bevacizumab or its biosimilar, combined with hepatic arterial interventional therapies (HAIT) in uHCC patients.

Influences of physical stimulations on the migration and differentiation of Schwann cells involved in peripheral nerve repair.

Peripheral nerve injury repair has always been a research concern of scientists. At the tissue level, axonal regeneration has become a research spotlight in peripheral nerve repair. Through transplantation of autologous nerve grafts or other emerging biomaterials functional recovery after facial nerve injury is not ideal in clinical scenarios.

Differential impact of TIM-3 ligands on NK cell function.

Background: The transmembrane protein T-cell immunoglobulin and mucin-domain containing molecule 3 (TIM-3) is an immune checkpoint receptor that is expressed by a variety of leukocyte subsets, particularly in the tumor microenvironment. An effective TIM-3-targeting therapy should account for multiple biological factors, including the disease setting, the specific cell types involved and their varying sensitivities to the four putative TIM-3 ligands (galectin-9, phosphatidylserine, high mobility group protein B1 and carcinoembryonic antigen cell adhesion molecule 1), each of which engages a unique binding site on the receptor's variable immunoglobulin domain. The primary objectives of this study were to assess the prevalence and function of TIM-3 natural killer (NK) cells in patients with head and neck squamous cell carcinoma (HNSCC), determine whether the four TIM-3 ligands differentially affect TIM-3 NK cell functions, identify the most immunosuppressive ligand, and evaluate whether targeting ligand-mediated TIM-3 signaling enhances NK cell effector functions.