Erucic acid impairs male fertility by suppressing retinoic acid synthesis in sertoli cells.

Although dietary factors are increasingly implicated as crucial determinants of male fertility, specific dietary risk factors and their metabolic mechanisms remain poorly understood. In this study, patients with non-obstructive azoospermia (NOA) or severe oligospermia (EO) demonstrated significantly elevated erucic acid (EA) levels, with a nearly 3-fold increase in serum (P < 0.0001) and a 27 % increase in semen (P = 0.

Association between chronic kidney disease and depression: evidence from the NHANES 2015-2018 and Mendelian randomization analysis.

Background: There was a link between depression and chronic kidney disease (CKD), yet causal relationship between them remains underexplored. This study was conducted to explore this relationship and offer fresh perspectives on how CKD affected depression risk.

Methods: Initially, 7537 subjects from National Health and Nutrition Examination Survey (NHANES) were categorized into depression and control groups based on Patient Health Questionnaire 9 (PHQ-9).

Global, regional, and national burden and trend of infertility and its subtypes from 1990 to 2021, with projections to 2035.

Purpose: To study the global, regional and national burden and trend of primary and secondary infertility, by sex and sociodemographic index (SDI), over the period 1990-2021.

Methods: Data on the prevalence and disability-adjusted life years (DALYs) due to infertility and its subtypes were retrieved from the GBD study 2021 for 204 countries and territories from 1990 to 2021. The counts and age-standardized rates are presented, along with their corresponding 95% uncertainty intervals (UIs).

Triclosan inhibits human trophoblast cell migration via TCF12-mediated RASSF8 regulation.

Triclosan (TCS), a fat-soluble broad-spectrum antimicrobial agent widely used in personal care products and medical disinfectants, has been linked to adverse reproductive outcomes including disrupted embryo implantation. The aim of the present study was to investigate the functional consequences and molecular mechanisms of TCS exposure on trophoblast cells, using the HTR-8/SVneo cell line as an established in vitro model of human extravillous trophoblasts. Exposure to environmentally relevant TCS concentrations (0-100 μM) revealed dose-dependent toxicity.

Preimplantation genetic testing for monogenic disorders to prevent MSH6 germline pathogenic variant related colorectal cancer.

Background: Colorectal cancer (CRC) is the third most deadly cancer in the world, accounting for approximately 10% of all cancer diagnoses and cancer-related deaths worldwide each year. In addition to recognized risk factors such as obesity, physical inactivity, and smoking, genetic predispositions significantly contribute to the risk of CRC.

Methods: Whole -exome sequencing (WES) was performed on a proband from a Chinese family diagnosed with CRC to identify potential variants.

Identification of Ferroptosis-Related Genes Associated With Cryptorchidism via Bioinformatics and Experimental Verification.

Cryptorchidism is a notorious innate malformation in children that always leads to oligospermatism or azoospermatism. Moreover, there is a relationship between oxidative stress and spermatogenesis dysfunction caused by cryptorchidism. Ferroptosis is associated with iron metabolism and oxidative stress as a novel form of cell death regulation, which is involved in the pathogenesis of many diseases.

Endometrial echo patterns of embryo transfer day affect pregnancy outcomes in frozen embryo transfer cycles: a retrospective clinical study.

Background: The echo pattern constitutes an ultrasonic index that reflects the characteristics of the endometrium across various phases of the menstrual cycle. However, consensus of endometrial echo pattern and pregnancy outcomes is lacking in clinical application.

Methods: The retrospective cohort study analyzed the data from the electronic records of women who underwent frozen embryo transfer (FET) with hormone replacement treatment (not only one cycle per patient) between July 2020 to August 2021 at Reproduction Medicine Center of Jbnminling Hospital of Medical College of Nanjing University.

A novel homozygous frameshift mutation in MCM8 causes primary gonadal dysgenesis in both genders.

Purpose: We aimed to demonstrate the genetic factors of primary gonadal dysgenesis in a consanguineous family characterized by underdeveloped testes and non-obstructive azoospermia (NOA) in a male and primary amenorrhoea and primary ovarian insufficiency (POI) in a female.

Methods: DNA was extracted from the male proband with infertility from the consanguineous family for whole-exome sequencing and bioinformatics analysis to screen for potential pathogenic genes and mutations. Sanger sequencing was used for further validation of his family pedigree.

Melatonin protects aged oocytes from depalmitoylation-mediated quality reduction by promoting PPT1 degradation and antioxidation.

Oocyte aging is closely related to a decline in female fertility, accompanied by increased reactive oxygen species levels and changes in protein posttranslational modifications. However, the role of protein palmitoylation in oocyte aging has not been investigated. In the present study, a new association between redox and palmitoylation in aging oocytes was found.

Multi-omics analysis and experimental verification reveal testicular fatty acid metabolism disorder in non-obstructive azoospermia.

Increasing evidence implicates disruptions in testicular fatty acid metabolism as a contributing factor in non-obstructive azoospermia (NOA), a severe form of male infertility. However, the precise mechanisms linking fatty acid metabolism to NOA pathogenesis have not yet been fully elucidated. Multi-omics analyses, including microarray analysis, single-cell RNA sequencing (scRNA-seq), and metabolomics, were utilized to investigate disruptions in fatty acid metabolism associated with NOA using data from public databases.

Exogenous 2,4-Epibrassinolide Alleviates Alkaline Stress in Cucumber by Modulating Photosynthetic Performance.

Brassinosteroids (BRs) are recognized for their ability to enhance plant salt tolerance. While considerable research has focused on their effects under neutral salt conditions, the mechanisms through which BRs regulate photosynthesis under alkaline salt stress are less well understood. This study investigates these mechanisms, examining plant growth, photosynthetic electron transport, gas exchange parameters, Calvin cycle dynamics, and the expression of key antioxidant and Calvin cycle genes under alkaline stress conditions induced by NaHCO.

Effects of Increasing the Nitrogen-Phosphorus Ratio on the Structure and Function of the Soil Microbial Community in the Yellow River Delta.

Nitrogen (N) deposition from human activities leads to an imbalance in the N and phosphorus (P) ratios of natural ecosystems, which has a series of negative impacts on ecosystems. In this study, we used 16s rRNA sequencing technology to investigate the effect of the N-P supply ratio on the bulk soil (BS) and rhizosphere soil (RS) bacterial community of halophytes in coastal wetlands through manipulated field experiments. The response of soil bacterial communities to changing N and P ratios was influenced by plants.

Effect of dialdehyde nanocellulose-tannin fillers on antioxidant, antibacterial, mechanical and barrier properties of chitosan films for cherry tomato preservation.

In this study, bio-based composite films from nanocellulose, tannin and chitosan were fabricated. First, tannin was covalently immobilized onto dialdehyde CNCs (DACNCs) through the nucleophilic reaction to obtain TA-CNCs. TA-CNCs were then added into chitosan matrix as the nanofillers to obtain chitosan-TA-CNC (CS-TA-CNC) films.

A novel homozygous mutation of CFAP300 identified in a Chinese patient with primary ciliary dyskinesia and infertility.

Primary ciliary dyskinesia (PCD) is a clinically rare, genetically and phenotypically heterogeneous condition characterized by chronic respiratory tract infections, male infertility, tympanitis, and laterality abnormalities. PCD is typically resulted from variants in genes encoding assembly or structural proteins that are indispensable for the movement of motile cilia. Here, we identified a novel nonsense mutation, c.

[Live birth achieved by oocyte donation in a patient with 45,X/46,XY mixed gonadal dysgenesis: A case report and literature review].

Objective: To investigate the etiology, diagnosis and treatment of 45,X/46,XY mixed gonadal dysgenesis and the patients' clinical characteristics of conception, pregnancy and delivery, with purpose of improving the treatment and pregnancy management of the patients.

Methods: We retrospectively analyzed the clinical data on a pregnant patient with 45,X/46,XY mixed gonadal dysgenesis.

Results: Based on the findings of hypoplasia of secondary sexual characteristics, streak gonads, chromosome karyotype incompatibility with social sex, and chromosome aberration in the gonadal tissue, the patient was diagnosed with 45,X/46,XY mixed gonadal dysgenesis, received oocyte donation and intracytoplasmic sperm injection-embryo transfer (ICSI-ET), and achieved a live birth.

A Novel Compound Heterozygous Mutation in TDRD9 Causes Oligozoospermia.

Oligozoospermia is an important cause of male infertility for which treatment options are limited. Spermatogenesis is complex, and the causes of oligozoospermia remain largely unknown. Because genetic mutations are important factors of oligozoospermia pathogenesis, our study aimed to explore the genetic causes of oligozoospermia.

Root growth characteristics and antioxidant system of in response to the short-term nitrogen and phosphorus addition in the Yellow River Delta.

Human activities have increased nitrogen (N) and phosphorus (P) inputs to the Yellow River Delta and the supply level of N and P affects plant growth as well as ecosystem structure and function directly. However, the root growth, stoichiometry, and antioxidant system of plants in response to N and P additions, especially for herbaceous halophyte in the Yellow River Delta (YRD), remain unknown. A field experiment with N addition (0, 5, 15, and 45 g N m yr, respectively) as the main plot, and P addition (0 and 1 g N m yr, respectively) as the subplot, was carried out with a split-plot design to investigate the effects on the root morphology, stoichiometry, and antioxidant system of .

Omega-3 polyunsaturated fatty acids and its metabolite 12-HEPE rescue busulfan disrupted spermatogenesis via target to GPR120.

Busulfan is an antineoplastic, which is always accompanied with the abnormal of spermatogonia self-renewal and differentiation. It has been demonstrated that the omega-3 polyunsaturated fatty acids (PUFAs) benefits mature spermatozoa. However, whether omega-3 can protect endogenous spermatogonia and the detailed mechanisms are still unclear.

A novel splicing mutation in helicase for meiosis 1 leads to non-obstructive azoospermia.

Purpose: Non-obstructive azoospermia (NOA) is an essential cause of male infertility for which treatment options are limited. The pathogenic mechanism of NOA, especially idiopathic NOA, remains unclear. Gene variations are associated with the occurrence of NOA.

Age-related elevation of O-GlcNAc causes meiotic arrest in male mice.

In recent years, the postponement of childbearing has become a critical social issue. Male fertility is negatively associated with age because of testis aging. Spermatogenesis is impaired with age, but the molecular mechanism remains unknown.

Plants changed the response of bacterial community to the nitrogen and phosphorus addition ratio.

Introduction: Human activities have increased the nitrogen (N) and phosphorus (P) supply ratio of the natural ecosystem, which affects the growth of plants and the circulation of soil nutrients. However, the effect of the N and P supply ratio and the effect of plant on the soil microbial community are still unclear.

Methods: In this study, 16s rRNA sequencing was used to characterize the response of bacterial communities in () rhizosphere and non-rhizosphere soil to N and P addition ratio.