Multiple recognition elements in biosensors: A review of antibiotic detection technologies.

Due to their bacteriostatic or bactericidal effects, antibiotics are widely used in the prevention and treatment of human and animal diseases. However, their irrational utilization has caused severe environmental pollution and threatened human health and safety through food chain. Given the critical limitations of traditional antibiotic detection methods, such as high costs, technical complexity, and time-consuming operations, it is essential to develop robust, accurate, sensitive, and field-deployable technologies for antibiotic detection.

Carbon Metabolism Modification in for Improving Fengycin Production and Investigating Antifungal Activity of Its Homologous Components.

As a lipopeptide, fengycin exhibits environmentally friendly, safe, and long-lasting biocontrol efficacy. However, due to its complex structure and the challenges in chemical synthesis, it is primarily produced through biosynthesis. This study reports an improvement in fengycin production in by engineering the central carbon metabolic pathway and blocking the carbon overflow pathway.

ComQXPA Quorum Sensing Dynamic Regulation Enhanced Fengycin Production of .

Fengycin is an antifungal drug that could be used as a biocontrol agent if it could be produced in high amounts. The ComQXPA quorum sensing (QS) system is a natural mechanism, regulating cell density-dependent behaviors in . This study employed the QS-targeted promoter to express the gene cluster in , coupling the ComQXPA system to produce fengycin.

Targeted knockout and plasmid-based transfer of NRPS/PKS for improving lipopeptide iturin A synthesis.

The nonribosomal peptide synthetase (NRPS)/polyketide synthase (PKS) assembly lines are a large enzymatic machinery that facilitates the transfer and synthesis of intermediates between multimodular megasynthases through complex protein-protein interactions. Although NRPS/PKS systems display a highly sophisticated biosynthetic pathway, a similar strategy for the holistic editing of these gene clusters has not been described. In this study, practical gene-editing tools were developed for long gene clusters, allowing the efficient knockout of entire NRPS/PKS gene clusters.

Multiple Strategies Enhance 7-Dehydrocholesterol Production from Kitchen Waste by Engineered .

7-Dehydrocholesterol (7-DHC) is an important precursor of vitamin D. The microbial synthesis of 7-DHC has attracted substantial attention. In this study, multiple strategies were developed to create a sustainable green route for enhancing 7-DHC yield from kitchen waste by engineered .

Improved engineered fungal-bacterial commensal consortia simultaneously degrade multiantibiotics and biotransform food waste into lipopeptides.

Resource utilization of food waste is necessary to reduce environmental pollution. However, antibiotics can enter the environment through food waste, resulting in antibiotic residues, which pose potential risks to human health. In this study, commensal artificial consortia were constructed through intercellular adaptation to simultaneously degrade antibiotics and bioconvert food waste into lipopeptides.

Integrated Biofilm Modification and Transcriptional Analysis for Improving Fengycin Production in Bacillus amyloliquefaciens.

Although fengycin exhibits broad-spectrum antifungal properties, its application is hindered due to its low biosynthesis level and the co-existence of iturin A and surfactin in Bacillus amyloliquefaciens HM618, a probiotic strain. In this study, transcriptome analysis and gene editing were used to explore the potential mechanisms regulating fengycin production in B. amyloliquefaciens.

Improving salt-tolerant artificial consortium of Bacillus amyloliquefaciens for bioconverting food waste to lipopeptides.

High-salt content in food waste (FW) affects its resource utilization during biotransformation. In this study, adaptive laboratory evolution (ALE), gene editing, and artificial consortia were performed out to improve the salt-tolerance of Bacillus amyloliquefaciens for producing lipopeptide under FW and seawater. High-salt stress significantly decreased lipopeptide production in the B.

Combinatorial metabolic engineering of Bacillus subtilis for de novo production of polymyxin B.

Polymyxin is a lipopeptide antibiotic that is effective against multidrug-resistant Gram-negative bacteria. However, its clinical development is limited due to low titer and the presence of homologs. To address this, the polymyxin gene cluster was integrated into Bacillus subtilis, and sfp from Paenibacillus polymyxa was expressed heterologously, enabling recombinant B.

Enhancing fengycin production in the co-culture of Bacillus subtilis and Corynebacterium glutamicum by engineering proline transporter.

Fengycin possesses antifungal activity but has limited application due to its low yields. Amino acid precursors play a crucial role in fengycin synthesis. Herein, the overexpression of alanine, isoleucine, and threonine transporter-related genes in Bacillus subtilis increased fengycin production by 34.

Improved Production of Fengycin in by Integrated Strain Engineering Strategy.

Fengycin is a lipopeptide with broad-spectrum antifungal activity. However, its low yield limits its commercial application. Therefore, we iteratively edited multiple target genes associated with fengycin synthesis by combinatorial metabolic engineering.

Mutualistic microbial community of Bacillus amyloliquefaciens and recombinant Yarrowia lipolytica co-produced lipopeptides and fatty acids from food waste.

Bioconversion is an important method for transforming food waste (FW) into high value-added products, rendering it harmless, and recycling resources. An artificial microbial consortium (AMC) was constructed to produce FW-based lipopeptides in order to investigate the strategy of FW bioconversion into value-added products. Exogenous fatty acids as a precursor significantly improved the lipopeptide production of Bacillus amyloliquefaciens HM618.

Artificial microbial consortium producing oxidases enhanced the biotransformation efficiencies of multi-antibiotics.

Antibiotic mixtures in the environment result in the development of bacterial strains with resistance against multiple antibiotics. Oxidases are versatile that can bio-remove antibiotics. Various laccases (LACs), manganese peroxidases (MNPs), and versatile peroxidase (VP) were reconstructed in Pichia pastoris.

Artificial consortia of Bacillus amyloliquefaciens HM618 and Bacillus subtilis for utilizing food waste to synthetize iturin A.

Food waste is a cheap and abundant organic resource that can be used as a substrate for the production of the broad-spectrum antifungal compound iturin A. To increase the efficiency of food waste biotransformation, different artificial consortia incorporating the iturin A producer Bacillus amyloliquefaciens HM618 together with engineered Bacillus subtilis WB800N producing lipase or amylase were constructed. The results showed that recombinant B.

Co-culture of Bacillus amyloliquefaciens and recombinant Pichia pastoris for utilizing kitchen waste to produce fengycins.

Kitchen waste (KW) is a vast potential source of fermentable substrates. To bio-convert the KW into high-value chemicals, we used KW as substrate for the production of fengycin by an artificial consortium containing Bacillus amyloliquefaciens HM618 producing fengycin and the engineering Pichia pastoris producing amylase, glucosidase, or lipases. The maximal amylase activity of the constructed amylase-producing engineering strain (recombinant P.

Fermentation optimization of surfactin production of Bacillus amyloliquefaciens HM618.

This work isolated a strain named Bacillus amyloliquefaciens HM618 from the soil, which can inhibit the growths of Botrytis cinerea, Rhizoctonia solani, and Escherichia coli DH5α. Based on the results of response surface methodology, the surfactin levels of strain HM618 were elevated from 0.724 to 1.

Improved the lipopeptide production of Bacillus amyloliquefaciens HM618 under co-culture with the recombinant Corynebacterium glutamicum producing high-level proline.

The application of antibacterial lipopeptides is limited by high cost and low yield. Herein, the exogenous L-proline significantly improved lipopeptide production by Bacillus amyloliquefaciens HM618. A recombinant Corynebacterium glutamicum producing high levels of proline using genetically modifying proB and putA was used to establish consortium, to improve lipopeptide production of strain HM618.

Biodegradation of sulfonamide antibiotics through the heterologous expression of laccases from bacteria and investigation of their potential degradation pathways.

In this study, seven laccase genes from different bacteria were linked with the signal peptides PelB, Lpp or Ompa for heterologous expression in E. coli. The recombinant strains were applied for the removal of sulfadiazine (SDZ), sulfamethazine (SMZ), and sulfamethoxazole (SMX).

Potential biotransformation pathways and efficiencies of ciprofloxacin and norfloxacin by an activated sludge consortium.

Fluoroquinolones (FQs), such as ciprofloxacin (CIP) and norfloxacin (NOR), are types of emerging trace pollutants that have attracted great attention. In this study, an activated sludge (AS) consortium with high bio-removal capability to CIP and NOR was obtained by acclimating with CIP and NOR for 10 d. Meanwhile, a CIP- and NOR- transforming bacterial strain (S5), which is highly homologous to the 16S rRNA gene sequence of Enterobacter sp.

Control of the polymyxin analog ratio by domain swapping in the nonribosomal peptide synthetase of Paenibacillus polymyxa.

Polymyxins are used as the last-line therapy against multidrug-resistant bacteria. However, their further clinical development needs to solve problems related to the presence of heterogeneous analogs, but there is still no platform or methods that can regulate the biosynthesis of polymyxin analogs. In this study, we present an approach to swap domains in the polymyxin gene cluster to regulate the production of different analogs.

Bio-removal of tetracycline antibiotics under the consortium with probiotics Bacillus clausii T and Bacillus amyloliquefaciens producing biosurfactants.

The contamination of the aquatic environments by tetracycline antibiotics (TCs) is an increasingly pressing issue. Here, we used the addition of exogenous surfactants and in situ biosynthesis of biosurfactants to remove tetracycline (TC), oxytetracycline (OTC), chlortetracycline (CTC), and their mixtures using the co-culture of probiotic Bacillus clausii T and Bacillus amyloliquefaciens HM618 producing surfactin. The addition of exogenous biosurfactants to remove TCs was superior to nonionic surfactants.

The biodegradation of cefuroxime, cefotaxime and cefpirome by the synthetic consortium with probiotic Bacillus clausii and investigation of their potential biodegradation pathways.

Cephalosporin residues in the environment are a great concern, but bioremediation options do exist. Bacillus clausii T reached a removal rate of 100% within 8 h when challenged with a mixture of cefuroxime (CFX), cefotaxime (CTX), and cefpirome (CPR). The co-culture of B.