Endoplasmic reticulum stress-related genes contribute to lung cancer risk: a multiomics data integration study.

Background: The role of endoplasmic reticulum stress (ERS) in lung cancer remains inadequately explored, with existing studies reporting conflicting results. This study aimed to investigate the causal relationships between ERS-related genes and lung cancer risk.

Methods: This study used two large-scale genome-wide association studies (GWAS) datasets on lung cancer and integrated multiomics data, including methylation, expression and protein quantitative trait loci, to determine the causal relationships between methylation, gene expression, protein abundance, and lung cancer risk via summary data-based Mendelian randomization (SMR) and colocalization analyses.

Novel HSPB8 mutations in severe early-onset myopathy with involvement of respiratory and cardiac muscles cause proteostasis defects in cell models.

Heat shock protein family B (small) member 8 (HSPB8) promotes chaperone-assisted selective autophagy (CASA), which assures proteostasis in muscles and neurons. HSPB8 frameshift mutations found in neuromyopathies are translated on the same frame, generating the same C-terminal extension, which causes HSPB8 aggregation and proteostasis defects. Here, we describe three novel HSPB8 frameshift variants, translated to protein using the third alternative frame to stop codons downstream to the canonical one and to the one used by other known HSPB8 frameshift mutants.

Analysis of intrinsic effects of compound curing agents on small molecule metabolites and microbial communities in Xuanwei ham based on metabolomics and high-throughput sequencing.

In order to reduce the salt content of Xuanwei ham and prevent the fading of the ham section, 18 hind legs of local black pigs were selected. The groups were cured with 6 % salt, 6 % compound curing agent I, and 6 % compound curing agent II, respectively. The differences in small molecule metabolites and microbial communities in Xuanwei ham among the groups were analyzed.

Impact of unilateral ureteral obstruction on cognition and neurodegeneration.

Introduction: Cognitive impairment is a common complication in chronic kidney disease (CKD) patients. Currently, limited types of animal models are available for studying cognitive impairment in CKD. We used unilateral ureteral obstruction (UUO) in mice as an animal model to study the cognitive changes and related pathology under prolonged renal impairment METHODS: UUO was performed in 8-week-old male C57BL/6 N mice with double-ligation of their left ureter.