Microglial-derived nitric oxide regulates amygdala synaptic plasticity to drive chronic pain and depression induced by lumbar disc herniation.

Lumbar disc herniation (LDH) is a major driver of chronic low back pain often accompanied by depression-like behaviors, yet the supraspinal mechanisms that link nociception to affective disturbance remain unclear. Here, we investigated the potential mechanisms by which microglia-derived nitric oxide (NO) modulates synaptic plasticity in the amygdala of a rat model of LDH. Behavioral assessments confirmed the presence of mechanical hyperalgesia and depression-like behaviors in LDH rats.

Microbiota composition-based donor selection affects FMT efficacy in a murine colitis model.

Background: Growing evidence links gut microbial dysbiosis to inflammatory bowel disease (IBD) pathogenesis, establishing fecal microbiota transplantation (FMT) as a microbiota-targeted therapy; however, variable outcomes in randomized trials highlight the need to identify compositional features of donor microbiota associated with FMT efficacy.

Objective: This study aimed to investigate how the composition of the donor gut microbiota influences the therapeutic efficacy of FMT in IBD.

Method: Fecal DNA from 39 IBD patients and 42 healthy donors was analyzed via 16S rRNA sequencing.

Induction immunochemotherapy followed by definitive chemoradiotherapy and consolidation immunotherapy for unresectable stage III non-small cell lung cancer: a multi-institutional retrospective cohort study.

Background: For unresectable stage III non-small cell lung cancer (NSCLC), the standard regimen is definitive concurrent chemoradiotherapy (CRT) followed by consolidation immunotherapy. We investigated whether incorporating induction immunochemotherapy enhances the efficacy.

Materials And Methods: From June 2018 to December 2022, 294 patients with unresectable stage III NSCLC were included, who did (162, I-CRT-I group) or did not (132, CRT-I group) receive induction immunochemotherapy, followed by definitive CRT and consolidation immunotherapy.

Anti-Inflammatory Secondary Metabolites from sp. NX-S-6.

Five new natural products, including two sorbicillinoids (-), one indolinone alkaloid (), one tetracyclic steroid (), and one α-pyrone derivative (), were identified from the endophytic sp. NX-S-6, together with thirteen known natural products. The structures of new compounds were unambiguously elucidated by comprehensive spectroscopic analyses (NMR, MS), as well as electronic circular dichroism (ECD) calculation.

Eutopic macrophages facilitate endometriosis progression via ferroptosis-mediated release of S100A9.

Endometriosis is a chronic inflammatory disorder in which immune cells, specifically macrophages, play a pivotal yet incompletely understood role. Aberrations within the eutopic endometrium are implicated in the initiation and progression of endometriosis. In this study, we reported a notable increase in the proportion of a distinct S100A9+ macrophage subpopulation undergoing ferroptosis in the eutopic endometrium of endometriosis patients compared with normal endometrium, as evidenced by single-cell RNA sequencing and experimental validation.

Antimycobacterial Muraymycins Isolated from sp. NRRL 30475 Using OSMAC and Precursor-Feeding Strategies.

Three mutant strains of sp. NRRL 30471 were screened with eight different media based on "One Strain Many Compounds" (OSMAC) and precursor-feeding strategies. Five new muraymycins, D5-D9 (-), together with three known congeners were isolated and identified from sp.

Mechanisms underlying the impacts of subtropical natural secondary succession on soil nitrogen mineralization.

To explore the changes in soil nitrogen (N) mineralization and underlying mechanisms during the succession of subtropical natural secondary forests, we employed a space-for-time substitution approach to study five forests at different developmental stages (, 5, 8, 21, 27, and 40 years), with the primary forests over 100 years old as a control. We measured soil net N mineralization rate under microcosm cultivation, as well as soil physi-cochemical properties, microbial biomass, and the richness of understory. The results showed that soil net N mine-ralization rates significantly increased with the succession of natural secondary forests, which varied from (-0.

Soil extracellular enzyme activities and their stoichiometric characteristics following the conversion of natural forests to plantation in subtropical region.

We investigated the responses of soil extracellular enzyme activities and stoichiometric characteristics following the conversion of mixed conifer-broadleaf forest to plantation in the subtropical region. The results showed that the conversion from natural forest to plantation significantly reduced activities of cellulose hydrolase (CBH), β-D-glucosidase (βG), β-N-acetylglucosaminidase (NAG), acid phosphatase (AP) by 70.6%, 53.

Enhanced Reaction Kinetics in Sodium-Ion Batteries Achieved by 3D Heterostructure CoS/CoS with Self-Induced Internal Electric Field.

The sluggish charging and restricted mass transfer of cobalt-based sulfides have provoked in cycling stability, poor rate, and low initial coulombic efficiency, impeding their practical application. Developing electronic configurations and heterostructures are effective methods to improve conductivity and accelerate mass transfer. In this work, heterostructured carbon/cobalt sulfides embedded in honeycomb-like nitrogen-doped carbon (HC@CoS/CoS/NC) were proposed as a cost-effective strategy.

Kaempferide enhances type I interferon signaling as a novel broad-spectrum antiviral agent.

Broad-spectrum antivirals (BSAs) possess unique advantages of being effective against a wide range of both existing and unpredictable emerging viral infections. The host type I interferon (IFN) response serves as a universal defense against diverse viral infections nonspecifically, providing attractive targets to develop novel BSAs. In this study, we identified the flavonoid kaempferide as an enhancer of the type I IFN activated Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway, promoting the expression of IFN stimulated genes (ISGs) and the establishment of cellular antiviral status.

Ectopic mouse TMC1 and TMC2 alone form mechanosensitive channels that are potently modulated by TMIE.

The mechanotransduction (MT) channel expressed in cochlear and vestibular hair cells converts the mechanical stimulation of sound and head movements into electrochemical signals. Recently, TMC1 and TMC2 (TMC1/2) have been recognized as the pore-forming subunit of the MT channel, but TMC1/2 functional expression in heterologous cells-which is critical for unequivocally identifying them as the bona fide pore-forming subunit of the MT channel-has not been achieved because ectopic TMC1/2 become trapped in the ER. Here, we report that adding a Fyn lipidation tag to mouse TMC1/2 (mTMC1/2) drove their cell-surface expression, and, importantly, full-length mTMC1/2 expressed alone functioned as mechanosensitive channels, underscoring the view that TMC1/2 constitute the sole pore-forming subunit of the MT channel.

Multi-Plant Concentrated Powder Improved Skin Whitening: A Double-Blinded, Randomized, and Placebo-Controlled Clinical Study.

Introduction: With the growing demand for skin-enhancing products in the market, research into edible plants has expanded significantly. Numerous studies have shown that plant extracts rich in phytochemicals can effectively improve skin issues such as wrinkles, pigmentation, and dullness. However, clinical studies focusing on the effects of combined ingredients are still limited.

Biodegradable Vanadium-Based Nanomaterials for Photothermal-Enhanced Tumor Ferroptosis and Pyroptosis.

The designability and high reactivity of nanotechnology provide strategies for antitumor therapy by regulating the redox state in tumor cells. Here, we synthesize a kind of vanadium dioxide nanoparticle encapsulated in bovine serum albumin and containing disulfide bonds (VSB NPs) for photothermal-enhanced ferroptosis and pyroptosis effects. Mechanism studies show that disulfide bonds can effectively consume overexpressed glutathione (GSH) in the tumor microenvironment, leading to a decrease in glutathione peroxidase 4 (GPX4) activity.

A stable open-shell peri-hexacene with remarkable diradical character.

[n]Peri-acenes ([n]PA) have attracted great interest as promising candidates for nanoelectronics and spintronics. However, the synthesis of large [n]PA (n > 4) is extremely challenging due to their intrinsic open-shell radical character and high reactivity. Herein, we report the successful synthesis and isolation of a derivative (1) of peri-hexacene in crystalline form.

Process-based modeling to reveal spatio-temporal variations of coastal wetland-mediated nutrient removal.

Nutrient pollution intensifies the strain on coastal ecosystems globally. Despite wetlands' significant nutrient removal potential, process-based assessments of nutrient removal in large-scale coastal wetlands face limitations that hinder accurate quantification of water quality improvements. This study proposes a novel quantitative approach by developing a coupled hydrodynamic-water quality-wetland biogeochemical model.

Quantitatively unveiling the role of coastal wetlands in regulating eutrophication and enhancing water environmental capacity.

Human activities have intensified the global challenge of coastal eutrophication. Recently, water resource managers have encountered difficulties in formulating precise pollutant reduction strategies to mitigate coastal eutrophication. Despite the recognized importance of coastal wetlands and pollution sources in influencing coastal nutrient levels, accurately quantifying their impact remains difficult.

AgNP Composite Silicone-Based Polymer Self-Healing Antifouling Coatings.

Biofouling poses a significant challenge to the marine industry, and silicone anti-biofouling coatings have garnered extensive attention owing to their environmental friendliness and low surface energy. However, their widespread application is hindered by their low substrate adhesion and weak static antifouling capabilities. In this study, a novel silicone polymer polydimethylsiloxane (PDMS)-based poly(urea-thiourea-imine) (PDMS-PUTI) was synthesized via stepwise reactions of aminopropyl-terminated polydimethylsiloxane (APT-PDMS) with isophorone diisocyanate (IPDI), isophthalaldehyde (IPAL), and carbon disulfide (CS).

A type of multifunctional cellulose nanocrystal composite silicone-based polymer coating for marine antibiofouling.

Nanocomposite polymer coatings are being used as a new generation of marine antibiofouling coatings because of their toxin-free chemical composition and ease of large-scale adoption. Cellulose nanocrystal (CN) exhibits significant potential for composite reinforcement. Herein, CN was surface-modified via α,ω-bis(3-(2-hydroxyl-terminated polydimethylsiloxane (HTPDMS), resulting in dihydroxyl-terminated poly(dimethylsiloxane)-grafted CN (HP-g-CN).

A broadly protective antibody targeting glycoprotein Gn inhibits severe fever with thrombocytopenia syndrome virus infection.

Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging bunyavirus that causes severe viral hemorrhagic fever and thrombocytopenia syndrome with a fatality rate of up to 30%. No licensed vaccines or therapeutics are currently available for humans. Here, we develop seven monoclonal antibodies (mAbs) against SFTSV surface glycoprotein Gn.

The 3d-4f electron transition of the CoS/CeO heterojunction for efficient oxygen evolution.

CoS/CeO, exhibiting the 3d-4f orbital coupling effect, is developed and shows exceptional OER activity, with an overpotential of 140 mV at 10 mA cm. DFT calculation and Raman spectra show the existence of a d-p-f electron transport ladder that can accelerate electron transfer through the Co-O(S)-Ce bond, optimize the adsorption free energy, and enhance the catalytic activity.

In Situ Electrochemical Synthesis of Squamous-like CuS Induced by Sulfate-Reducing Bacteria as a Fenton-like Catalyst in Wastewater Treatment: Catalytic Performance and Mechanism.

In this paper, a novel method was proposed for the synthesis of CuS on copper mesh via electrolysis in SRB culture medium. It was found that following electrolysis in SRB medium, squamous-like CuS arrays were obtained on the copper mesh, and the CuS loading contents varied with the electrolyzing parameters. The resultant CuS on copper mesh in SRB (CSCM-SRB) with the highest catalytic MB degradation properties was produced by electrolysis at 3.

Ultrasound assisted electrodeposition of photocatalytic antibacterial MoS-Zn coatings controlled by sodium dodecyl sulfate.

Photocatalytic MoS with visible light response is considered as a promising bactericidal material owing to its non-toxicity and high antibacterial efficiency. However, photocatalysts always exist as powder, so it is difficult to settle photocatalysts on the metal surface, which limits their application in aqueous environments. To solve this problem, ultrasound and sodium dodecyl sulfate (SDS) were introduced into the co-deposition process of MoS and zinc matrix, so that novel MoS-Zn coatings were obtained.

Incorporating a built-in electric field into a NiFe LDH heterojunction for enhanced oxygen evolution and urea oxidation.

Herein, a N-doped carbon-supported Co and NiFe LDH (Co-NC@NiFe LDH) array was developed, which demonstrated superior catalytic activities for both the OER and UOR in an alkaline medium. The intrinsic electron transfer is effectively regulated by the construction of a built-in electric field, which reduces the reaction energy barrier and consequently leads to a significant enhancement in electrocatalytic activity.