Comprehensive Molecular and Epidemiological Characterization of Isolated from Bovine Mastitis in Water Buffalo of the Peshawar Division, Khyber Pakhtunkhwa, Pakistan.

Water buffalo () are a primary source of milk in Pakistan, where bovine mastitis is a significant health issue among cattle, leading to substantial economic losses. is a predominant pathogen associated with mastitis; however, a detailed molecular characterization of the strains in the country remains limited. We previously characterized mastitis strains from the Hazara division of Khyber Pakhtunkhwa, Pakistan.

The 2024 monkeypox outbreak in Pakistan: assessing the nation's public health preparedness.

The new subclade Ib of the monkeypox virus (MPXV) is spreading rapidly across Africa and has now been reported outside the continent. Historically, clade I has been associated with more severe disease than clade II, and its newly mutated variant, clade Ib is raising further concerns. Pakistan is among the first non-African countries to confirm a case of mpox in the recent outbreak, amid the World Health Organization's sounding warning of a public health emergency of international concern (PHEIC).

Performance of disc diffusion and gradient strip test on different Mueller-Hinton agar media plates and ComASP® and Bruker UMIC® Cefiderocol Microdilution Panels for cefiderocol susceptibility testing in carbapenem-resistant Pseudomonas aeruginosa.

Background: Cefiderocol antimicrobial susceptibility testing requires standardized iron levels to produce consistent results. Here we assessed the analytical performances of two commercialized broth microdilution (BMD) methods (Bruker's UMIC and Liofilchem's ComASP), of disc diffusion (DD) and the gradient strip method on a collection of clinical carbapenem-resistant-Pseudomonas aeruginosa (CRPA) isolates.

Methods: A collection of 117 WGS-CRPA was used.

Mortality Trends Associated With Acute Myocardial Infarction and Psychoactive Substance Use in Older Adults: A US Nationwide Analysis (1999-2020).

Background: Acute myocardial infarction (AMI) remains a leading cause of mortality in the USA, particularly among individuals aged 65 and older. There is limited research about the association between psychoactive substance use and cardiovascular death due to AMI. This study aims to analyze trends in AMI-related mortality among older adults (aged ≥ 65) associated with psychoactive substance use in the USA from 1999 to 2020, with a focus on demographic and geographic variations.

Does an improved HDI trigger tourism outflows for China? New evidence from the ARDL cointegration approach.

Extant studies have predominantly focused on understanding the effects of inbound tourism on economic growth. At the same time, it ignores the key factors that promote outbound tourism in a country. Outbound tourism not only plays a crucial role in the sustainable development of the host country but also helps foster an understanding of cross-cultural similarities and differences, promoting goodwill towards the home country.

Trends in Cardiovascular Mortality in Patients With Chronic Kidney Disease From 1999 to 2020: A Retrospective Study in the United States.

Background: Chronic kidney disease (CKD) may be associated with fatal cardiovascular diseases (CVDs). We aim to identify CVD-related mortality trends in patients with CKD in the US, examining the variation by sex, race, and region, and compare them to CVD-related mortality trends in general.

Methods: The CDC-WONDER database was used to obtain age-adjusted mortality rates (AAMRs) per 100,000 population.

Phytoremediation potential of native medicinal plant species in mining degraded soil of Mohmand Northwest Pakistan.

The phytoremediation efficiency of sixteen native medicinal plants was studied in soil degraded by mining in Mohmand, Northwest Pakistan. Elemental analyses of samples were carried out by using an atomic absorption spectrophotometer. The average values of soil pH (7.

Chlorophyllum molybdites-synthesized manganese oxide nanoparticles (MnO-NPs): morphology, biocompatibility, and anticancer properties against liver cancer (HepG2) cell line.

Nanoparticles synthesized from natural sources are gaining prominence for their unique physicochemical properties and biocompatibility. This study investigates the potential of wild mushrooms, specifically Chlorophyllum molybdites (Lepiota morganii, green-spored parasol), for the green synthesis of manganese oxide nanoparticles. Mycosynthesized nanoparticles (NPs) were characterized via spectroscopic techniques such as XRD, UV spectroscopy, FTIR, and microscopic techniques such as SEM, and EDX, confirming their crystalline structure, spherical morphology, and a nanoscale size of 19.

Ecological and health risks assessment of potentially toxic elements in edible plants across mining and non-mining areas of Northwest, Pakistan.

The current study evaluated the concentrations, ecological and health risks, and phytoremediation potential of potentially toxic elements (PTEs) in soil and edible plant samples from mining and non-mining areas of District Mohmand, Pakistan. Soil and plant samples were collected, oven-dried, acid-digested (HNO:HCl, 3:1) and analyzed using atomic absorption spectrophotometry (Perkin-Elmer AA400). Traditionally, many of the studied plant species serve both as food and in ethnomedicines.

Revealing the Micro-Size Effect in Alloy Anodes for High-Capacity and Long-Cycling Sulfide-Based Solid-State Batteries.

Solid-state batteries (SSBs) are competitive contenders for energy storage due to their inherent safety and high energy. However, the lack of an appropriate anode has hindered their development. Graphite and lithium metal are widely used anode materials, but graphite suffers from a low capacity, whereas lithium metal presents severe dendrite and reactivity challenges.

Exploring the rising death rates among older US citizens with heart failure and sepsis: Need for health-care policy reform.

Background: Heart failure (HF) patients, often multimorbid and immunocompromised, are particularly vulnerable to sepsis, which increases their risk for mortality. It is therefore crucial to understand the mortality patterns in older adults (≥65) with HF and sepsis for improving care strategies in the United States.

Methods: A cross-sectional evaluation of death certificates from the Centers for Disease Control and Prevention's Wide-Ranging Online Data for Epidemiologic Research (CDC WONDER) was conducted to examine HF and sepsis mortality trends.

Reproductive health crisis amidst a natural disaster in Pakistan: A call to action.

In 2022, Pakistan and the South Asian region experienced unprecedented natural disasters. The country was devastated by severe floods caused by unusually high monsoon rains and river overflows. The Pakistani government reported that at least 80 districts nationwide were impacted, leading to the loss of one million homes, over 1000 fatalities, and 1600 injuries.

Trends in atrial fibrillation and flutter mortality associated with disorders of thyroid gland in the United States from 1999 to 2020.

Background: Thyroid gland disorders are a known risk factor for atrial fibrillation and flutter (AF/AFL). Despite being a well-established risk factor, most studies have primarily examined prevalence, comorbidities, and treatment patterns with little to no research on mortality trends for this association. Objective: We aimed to analyze the trends in AF/AFL-related mortality in patients with thyroid gland disorders.

Safety and efficacy of pemigatinib in patients with cholangiocarcinoma: a systematic review.

Background: Cholangiocarcinoma (CCA) is an aggressive bile duct cancer with limited therapeutic options and poor prognosis. pemigatinib, a selective inhibitor, has emerged as a promising targeted therapy for CCA patients harboring fusions or rearrangements. This systematic review evaluated the safety and efficacy of pemigatinib in this patient population.

Comparison of mortality trends in patients with rheumatic mitral valve disease and nonrheumatic mitral valve disease: A retrospective study in US from 1999 to 2020.

Background: Mitral valve disease (MVD) can have both rheumatic and nonrheumatic etiologies. However, differences in mortality remain unknown.

Methods: We extracted age-adjusted mortality rates (AAMRs) per 100,000 persons from the CDC WONDER database and stratified them by sex, region, and race.

Diagnostic algorithm for the detection of carbapenemases and extended-spectrum β-lactamases in carbapenem-resistant .

can acquire carbapenem resistance through various mechanisms, including genomic mutations leading to the overexpression of efflux pumps, intrinsic AmpC-β-lactamase, and/or reduced permeability, and/or through the acquisition of plasmid-mediated carbapenemases and/or extended-spectrum-β-lactamases (ESBLs). Unfortunately, carbapenemase/ESBL-producing-carbapenem-resistant- (CP/ESBL-CRPA) cannot be differentiated from non-CP/ESBL-CRPA based solely on susceptibility testing results of conventional β-lactam (BL)-antibiotics. Knowing that these two groups display different activity profiles toward novel BL/β-lactamase-inhibitor (BLI) combinations, we developed and verified a cost-effective and easy-to-use diagnostic algorithm for screening and differentiation of carbapenemase and ESBL production in CRPA.

Triangular Lunulae in Papillon-Lefèvre Syndrome: A Case Report.

Triangular lunulae, typically associated with Nail-Patella Syndrome, may also present in other syndromes, as seen in this case. Papillon-Lefèvre syndrome is a rare genodermatosis linked to significant morbidity. Consanguinity is a recognized high-risk factor.

Treatment and long-term outcome of mental disorders: The grim picture from a quasi-epidemiological investigation in 54,826 subjects from 40 countries.

Introduction: This study registered rates of specific treatment options for mental disorders as well as their long-term outcome.

Material And Methods: The history of mental disorders was used as a proxy for diagnosis. The data came from the COMET-G study (40 countries; 54,826 subjects, 64.

Smallpox Vaccines for Monkeypox: Is Emergency Vaccination Imminent?

Monkeypox virus (MPXV) is a contagious disease that has been endemic in central and west Africa since 1970, characterized by symptoms such as fever, headache, and skin rash. While there is no approved treatment for MPXV infections, vaccination has proven effective in limiting its transmission, and previous smallpox vaccinations may also provide protection against monkeypox. However, the dependence of monkeypox on animal hosts makes eradication more complicated than with smallpox.

Recovery, modifications, physicochemical properties, and MB removal efficiency of marble waste powder.

A large quantity of marble waste is produced worldwide which imposes serious environmental problems. The marble rocks are made of valuable inorganic oxides and carbonates. Unfortunately, the recovery of marble waste is only limited to mechanical uses.

Revolutionizing electrocardiography: the role of artificial intelligence in modern cardiac diagnostics.

Electrocardiography (ECG) remains a cornerstone of non-invasive cardiac diagnostics, yet manual interpretation poses challenges due to its complexity and time consumption. The integration of Artificial Intelligence (AI), particularly through Deep Learning (DL) models, has revolutionized ECG analysis by enabling automated, high-precision diagnostics. This review highlights the recent advancements in AI-driven ECG applications, focusing on arrhythmia detection, abnormal beat classification, and the prediction of structural heart diseases.

Impact of geo-climatic factors on the prevalence and diversity of tick fauna in Achai cattle of the northern Hindukush Mountains, Khyber pakhtunkhwa, Pakistan.

Background: Ticks are blood-feeding ectoparasites that can transmit different diseases in livestock and humans. Globally, 994 tick species are recognized, belonging to three families, that is, Argasidae (220 species), Ixodidae (773 species) and Nuttalliellidae (one species). The current study investigated the impact of geo-climatic conditions on the prevalence and distribution of ticks in Achai cattle of the northern Hindukush Mountains of Khyber Pakhtunkhwa.

Gut microbiota therapy in gastrointestinal diseases.

The human gut microbiota, consisting of trillions of microorganisms, plays a crucial role in gastrointestinal (GI) health and disease. Dysbiosis, an imbalance in microbial composition, has been linked to a range of GI disorders, including inflammatory bowel disease (IBD), irritable bowel syndrome (IBS), celiac disease, and colorectal cancer. These conditions are influenced by the interactions between the gut microbiota, the host immune system, and the gut-brain axis.

Concentrated Chloride Electrolytes Enable High-Efficiency, Long-Cycling, and Dendrite-Free Aqueous Trivalent Antimony Batteries.

Aqueous trivalent metal batteries are promising energy storage systems, which can leverage unique three-electron redox reactions to deliver high capacity and high energy. Among them, antimony (Sb) stands out with a high capacity (660 mAh g), abundant availability, and low cost. However, the severe Sb hydrolysis reaction drastically hinders the development of aqueous antimony batteries.

Ethnobotanical knowledge and ethnomedicinal uses of plant resources by urban communities of Khyber Pakhtunkhwa, Pakistan: a novel urban ethnobotanical approach.

Study Background: Ethnobotanical knowledge of plants in rural and remote areas is more common in Pakistan than in urban areas. This study was carried out to find the indigenous knowledge of plants in anthropogenically disturbed urban areas of Khyber Pakhtunkhwa, Pakistan.

Materials And Methods: Eight visits (Two per season) were carried out from March 2023 to March 2024.