Deep learning-based colorimetric indicator on polylactic acid packaging for nondestructive monitoring of fresh-cut fruits and vegetables.

The perishability of fruits and vegetables (F&v) presents a significant challenge in maintaining food quality and safety. However, current methods for monitoring the freshness of fresh-cut F&v remains limited. This study introduces a novel deep learning-based colorimetric indicator system designed for the nondestructive monitoring of freshness in fresh-cut F&v packed in polylactic acid (PLA) bags.

Aroma-Driven Differentiation of Wuyi Shuixian Tea Grades: The Pivotal Role of Linalool Revealed by OAV and Multivariate Analysis.

Wuyi Shuixian tea, a premium oolong tea known for its complex floral-fruity aroma, exhibits significant quality variations across different grades. This study systematically analyzed the aroma characteristics and key fragrant compounds of four grades (Grand Prize SA, First Prize SB, Outstanding Award SC, and Non-award SD) using headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS), odor activity value (OAV) analysis, and multivariate statistical methods. A total of 159 volatile compounds were identified, with similar compound categories but distinct concentration gradients between grades.

Enhancing bullfrog farming sustainability: circular water management through effluent treatment.

Ensuring effective wastewater treatment is crucial for promoting sustainable water use and reducing environmental pollution in intensive bullfrog aquaculture. This study presents the design and implementation of an integrated treatment system, composed of a flotation tank, a biochemical tank, and constructed wetlands, aimed at facilitating the reuse of treated effluent. The system, operated under optimal conditions-at a flow rate of 250 m/h, hydraulic retention time of 6 h, and aeration intensity of 2,000 m/h-demonstrated significant removal efficiencies.

MOGAN for LUAD Subtype Classification by Integrating Three Omics Data Types.

Background: Lung adenocarcinoma (LUAD) is a highly heterogeneous cancer type with a poor prognosis. Accurate subtype identification can help guide its treatment. The traditional subtype identification methods using a single-omics approach make it difficult to comprehensively characterize the molecular features of LUAD.

Structure-function analysis of 2-sulfamoylacetic acid synthase in altemicidin biosynthesis.

Altemicidin and its analogs are valuable sulfonamide antibiotics with valuable antitumor and antibacterial activities. Structures of altemicidin and congeners feature an unusual sulfonamide side chain. In the biosynthesis of altemicidin, the aldehyde dehydrogenase SbzJ catalyzes the conversion of 2-sulfamoylacetic aldehyde into 2-sulfamoylacetic acid, a key step in producing the sulfonamide side chain.

Impact of tidal fluctuations on bacterial community structure in Wuyuan Bay: A comparative analysis of waters inside and outside the tidal barrage.

The tidal barrage at Wuyuan Bay effectively mitigated the odor from the tidal flat during ebb tide, however, its effect on bacterial community structure in waters are still unclear. In this study, high-throughput sequencing was used to analyze the structure of the microbial community in waters inside and outside the tidal barrage during flood and ebb tides. Results showed bacterial diversity was higher in water outside the barrage during flood tide.

PcAvh87, a virulence essential RxLR effector of Phytophthora cinnamomi suppresses host defense and induces cell death in plant nucleus.

Plants have developed intricate immune mechanisms to impede Phytophthora colonization. In response, Phytophthora secretes RxLR effector proteins that disrupt plant defense and promote infection. The specific molecular interactions through which Phytophthora RxLR effectors undermine plant immunity, however, remain inadequately defined.

Integrated Single-Cell and Transcriptome Sequencing Analyses Develop a Ubiquitination-Associated Signature in Gastric Cancer and Identified OTULIN as a Novel Biomarker.

Background: Gastric cancer (GC) is the most commonly diagnosed digestive system malignancy with a dismal survival outcome. The prognostic value of ubiquitination-related genes (URGs) in GC has yet to be discovered.

Methods: Two GC cohort datasets were obtained from the Cancer Genome Atlas Program (TCGA) and Gene Expression Omnibus (GEO) databases.

Soil metagenomic analysis on changes of functional genes and microorganisms involved in nitrogen-cycle processes of acidified tea soils.

Nitrogen (N) is the first essential nutrient for tea growth. However, the effect of soil acidification on soil N cycle and N forms in tea plantation are unclear. In this study, the nitrogen contents, soil enzyme activity and N mineralization rate in acidified soil of tea plantation were measured.

Rapid detection of based on a new target gene .

causes crown and root wilting in more than 5,000 plant species and represents a significant threat to the health of natural ecosystems and horticultural crops. The early and accurate detection of is a fundamental step in disease prevention and appropriate management. In this study, based on public genomic sequence data and bioinformatic analysis of several , , and species, we have identified a new target gene, Pcinn13739; this allowed us to establish a recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) assay for the detection of .

Circular RNA circTTBK2 facilitates non-small-cell lung cancer malignancy through the miR-873-5p/TEAD1/DERL1 axis.

The underlying mechanisms by which circular RNAs (circRNAs) regulate non-small-cell lung cancer (NSCLC) progression remain elusive. This study investigated the role of circRNA circTTBK2 in NSCLC tumorigenesis. Quantitative reverse transcriptase polymerase chain reaction analysis of circTTBK2 in NSCLC tissues and cell lines was performed.

Spatial-Temporal Distribution of Allelopathic Rice Roots in Paddy Soil and Its Impact on Weed-Suppressive Activity at the Seedling Stages.

Background: Allelochemicals secreted by allelopathic rice roots are transmitted to the receptor rhizosphere through the soil medium to inhibit the growth of the surrounding weeds. This research aimed to explore the relationships between the spatial-temporal distribution of rice roots in soil and weed-suppression ability at its seedling stage.

Results: This study first examined the root distribution of three rice cultivars in paddy soil in both vertical and horizontal directions at 3-6 leaf stage.

Identification of 9 Gene Signatures by WGCNA to Predict Prognosis for Colon Adenocarcinoma.

Background: A risk assessment model for prognostic prediction of colon adenocarcinoma (COAD) was established based on weighted gene co-expression network analysis (WGCNA).

Methods: From the Cancer Genome Atlas (TCGA) database, RNA-seq data and clinical data of COAD patients were retrieved. After screening of differentially expressed genes (DEGs), WGCNA was performed to identify gene modules and screen those associated with COAD progression.

Expression and Prognosis Value of the KLF Family Members in Colorectal Cancer.

Krüppel-like factors (KLFs) are some kind of transcriptional regulator that regulates a broad range of cellular functions and has been linked to the development of certain malignancies. KLF expression patterns and prognostic values in colorectal cancer (CRC) have, however, been investigated rarely. To investigate the differential expression, predictive value, and gene mutations of KLFs in CRC patients, we used various online analytic tools, including ONCOMINE, TCGA, cBioPortal, and the TIMER database.

Hypoxia-inducible long noncoding RNA NPSR1-AS1 promotes the proliferation and glycolysis of hepatocellular carcinoma cells by regulating the MAPK/ERK pathway.

Hepatocellular carcinoma (HCC), which accounts for approximately 90% of primary liver cancer, is commonly treated with surgical resection. However, most patients lose the opportunity to receive this therapeutic strategy due to delayed diagnosis and rapid tumor progression. Long noncoding RNAs (lncRNAs) have been demonstrated to play essential roles in the initiation and progression of HCC.

Enzymatic Synthesis of Puerarin Glucosides Using Cyclodextrin Glucanotransferase with Enhanced Antiosteoporosis Activity.

Puerarin () is the most abundant isoflavone from the root of and exhibits a broad range of pharmacological activities. However, poor water solubility and low bioavailability limit its use. Enzymatic transglycosylation is emerging as a new strategy to improve the pharmacodynamic and pharmacokinetic properties of natural products for drug development.

Effectiveness associated with different therapies for senile osteoporosis: a network Meta-analysis.

Objective: The aim of this study was to evaluate the effectiveness of Chinese herbal medicines for invigorating the kidney (CHMIK) on senile osteoporosis.

Methods: We searched for studies in English-language databases (PubMed, the Cochrane Library, and Web of Science) and Chinese-language databases (China National Knowledge Infrastructure, Wan Fang Data, VIP Chinese periodical service platform, and China Biology Medicine disc from their inception to September 2017. Randomized controlled trials comparing the effectiveness of Traditional Chinese Medicine therapies (alone or in combination) and conventional clinical medicine therapies among older adult patients with osteoporosis were identified.

Analysis of the absorption and accumulation characteristics of rare earth elements in Chinese tea.

Background: Rare earth elements (REEs) played an important role in enhancing the yield and quality of tea, and have been widely used in tea cultivation. In the present study, 3011 tea samples of four tea types, namely black tea, green tea, oolong tea and dark tea, were collected from the mainly tea producing areas in different provinces of China to analyze the contents of 16 REEs by inductively coupled plasma mass spectrometry.

Results: The results obtained showed that the higher average content of REEs was 2.

MYB57 transcriptionally regulates MAPK11 to interact with PAL2;3 and modulate rice allelopathy.

Rice allelopathy is a natural method of weed control that is regarded as an eco-friendly practice in agroecology. The allelopathic potential of rice is regulated by various genes, including those that encode transcription factors. Our study characterized a MYB transcription factor, OsMYB57, to explore its role in the regulation of rice allelopathy.

Fine-root traits of allelopathic rice at the seedling stage and their relationship with allelopathic potential.

Background: Allelopathic rice releases allelochemicals through its root systems, thereby exerting a negative effect on paddy weeds. This research aimed to evaluate the relationship between fine-root traits and the rice allelopathic potential at the seedling stage.

Methods: Two allelopathic rice cultivars, 'PI312777' and 'Taichung Native1,' and one non-allelopathic rice cultivar, 'Lemont,' were grown to the 3-6 leaf stage in a hydroponic system.

MiR-210-3p inhibits osteogenic differentiation and promotes adipogenic differentiation correlated with Wnt signaling in ERα-deficient rBMSCs.

MicroRNAs (miRNAs) regulate activities in living organisms through various signaling pathways and play important roles in the development and progression of osteoporosis. The balance between osteogenic and adipogenic differentiation of rBMSCs is closely related to the occurrence of osteoporosis. ERα regulates bone metabolism in various tissues.

Transcriptome analysis reveals that barnyard grass exudates increase the allelopathic potential of allelopathic and non-allelopathic rice (Oryza sativa) accessions.

Background: Allelopathy in rice (Oryza sativa) is a chemically induced response that is elevated by the exogenous application of chemical compounds and barnyard grass root exudates. An in-depth understanding of the response mechanisms of rice to chemical induction is necessary for the identification of target genes for increasing the allelopathic potential of rice. However, no previous studies have evaluated the transcriptomic changes associated with allelopathy in rice in response to barnyard grass exudates treatment.