Comparative physiological and cytological analysis, and omics approach provide clues to the coloring mechanism of the pumpkin yellow stems.

Background: The abnormal chloroplast and pigment accumulation could lead to plant yellowing. However, the plant tissue chlorisis is species-specific and could display various phenotypes due to the genetic and environmental impacts. The molecular mechanisms underlying the plant stem yellowing are less understood than the flower or leaf coloring mechanisms.

Genome-wide analysis of respiratory burst oxidase homolog gene family in pea ( L.).

Plant respiratory burst oxidase homologs (RBOHs) are key enzymes regulating superoxide production, which is important for plant development and responses to biotic and abiotic stresses. This study aimed to characterize the gene family in pea ( L.).

Candidate regulators of drought stress in tomato revealed by comparative transcriptomic and proteomic analyses.

Drought is among the most common abiotic constraints of crop growth, development, and productivity. Integrating different omics approaches offers a possibility for deciphering the metabolic pathways and fundamental mechanisms involved in abiotic stress tolerance. Here, we explored the transcriptional and post-transcriptional changes in drought-stressed tomato plants using transcriptomic and proteomic profiles to determine the molecular dynamics of tomato drought stress responses.

Comprehensive Analysis to Reveal Amino Acid Metabolism-Associated Genes as a Prognostic Index in Gastric Cancer.

Background: Amino acid metabolism (AAM) is related to tumor growth, prognosis, and therapeutic response. Tumor cells use more amino acids with less synthetic energy than normal cells for rapid proliferation. However, the possible significance of AAM-related genes in the tumor microenvironment (TME) is poorly understood.

Metabolic and molecular mechanisms underlying the foliar Zn application induced increase of 2-acetyl-1-pyrroline conferring the 'taro-like' aroma in pumpkin leaves.

Introduction: Fresh pumpkin leaf is popular vegetable for its rich nutrition. The pleasant taro-like odour is important aroma quality of crops, and mostly contributed by 2-acetyl-1-pyrroline in pumpkin. Element Zn can impact metabolite biosynthesis in plants, including aroma formation.

QTL mapping reveals candidate genes for main agronomic traits in based on a high-resolution genetic map.

is an important medicinal and edible vegetable crop of Cucurbitaceae. Strong heterosis effects and strikingly complementary characteristics were found between the two domesticated cultivars, and . To explore the genetic basis underlying their important agronomic traits, we constructed the first interspecific high-density genetic linkage map using a BC population of 110 lines derived from a cross between S1174 () and P93075 ().

Assessment of the 'taro-like' aroma of pumpkin fruit ( D.) via E-nose, GC-MS and GC-O analysis.

'Taro-like' aroma is a pleasant flavor and value-added trait in pumpkin species imparted by unknown key volatile compounds. In this study, we used the electronic nose (E-nose), gas chromatography-mass spectrometry (GC-MS), and GC-Olfactometry (GC-O) to study the aroma profile, volatile compounds, and key contributors, respectively. By E-nose and GC-MS, we found significant differences in the aroma profiles and volatile compounds between fruits from five samples with/without 'taro-like' aroma.

Metabolome and Transcriptome Analyses of Cucurbitacin Biosynthesis in ().

Cucurbitacins are extremely bitter compounds mainly present in Cucurbitaceae, where belongs. However, there is no comprehensive analysis of cucurbitacin biosynthesis in fruit. Therefore, this study analyzed bitter (WM709) and non-bitter (S1174) genotypes of to reveal the underlying mechanism of cucurbitacin biosynthesis by integrating metabolome and transcriptome analyses.

Identification of suitable reference genes for quantitative reverse transcription PCR in ().

Unlabelled: Reverse transcription real-time quantitative PCR is widely used to quantify gene expression. Reference genes are usually used as internal controls to measure the target gene expression level. To date, there is no consensus on the use of systematically validated reference genes in different tissues of .

A mutation in LacDWARF1 results in a GA-deficient dwarf phenotype in sponge gourd (Luffa acutangula).

A dwarfism gene LacDWARF1 was mapped by combined BSA-Seq and comparative genomics analyses to a 65.4 kb physical genomic region on chromosome 05. Dwarf architecture is one of the most important traits utilized in Cucurbitaceae breeding because it saves labor and increases the harvest index.

Dysregulated Expression Levels of Circular RNAs Serve as Prognostic and Clinicopathological Markers in Gastric Cancer: a Meta-Analysis.

Background: Circular RNAs (circRNAs) are novel biomarkers that are widely investigated in various cancers. There is increasing evidence that the expression levels of circRNAs are upregulated or downregulated in various cancers, but the overall prognostic efficiency of circRNAs in gastric cancer (GC) remains unclear. Therefore, this meta-analysis studies the relationship between circRNA expression and the prognosis of gastric malignancies.

A high-quality sponge gourd () genome.

Sponge gourd () is an important cultivated vegetable and medicinal plant in the family Cucurbitaceae. In this study, a draft genome sequence of the sponge gourd inbred line P93075 was analyzed. Using Illumina, PacBio, and 10× Genomics sequencing techniques as well as new assembly techniques such as FALCON and chromatin interaction mapping (Hi-C), a chromosome-scale genome of approximately 656.

Identification of drought-responsive microRNAs in tomato using high-throughput sequencing.

Drought is a major abiotic stress affecting crop productivity and quality. As a class of noncoding RNA, microRNA (miRNA) plays important roles in plant growth, development, and stress response. However, their response and roles in tomato drought stress is largely unknown.

Profiling of drought-responsive microRNA and mRNA in tomato using high-throughput sequencing.

Background: Abiotic stresses cause severe loss of crop production. Among them, drought is one of the most frequent environmental stresses, which limits crop growth, development and productivity. Plant drought tolerance is fine-tuned by a complex gene regulatory network.