Erratum: Inertial Migration in a Pressure-Driven Channel Flow: Beyond the Segre-Silberberg Pinch [Phys. Rev. Lett. 132, 054002 (2024)].

This corrects the article DOI: 10.1103/PhysRevLett.132.

Four Years in, What Are the Research Priorities for Long COVID? A Research Priority-Setting Partnership Between People With Lived Experience, Carers, Clinicians and Researchers.

Introduction: Long COVID is a life-limiting condition that affects 65 million people worldwide. It devastates lives with uncertain illness trajectories, and yet, there are many research uncertainties as there is a lack of understanding of its causes, effective treatments and management plans. We set out to identify current research priorities for people with Long COVID, carers, healthcare professionals and researchers.

Allopurinol and blood pressure variability following ischemic stroke and transient ischemic attack: a secondary analysis of XILO-FIST.

Blood Pressure Variability (BPV) is associated with cardiovascular risk and serum uric acid level. We investigated whether BPV was lowered by allopurinol and whether it was related to neuroimaging markers of cerebral small vessel disease (CSVD) and cognition. We used data from a randomised, double-blind, placebo-controlled trial of two years allopurinol treatment after recent ischemic stroke or transient ischemic attack.

Inertial Migration in a Pressure-Driven Channel Flow: Beyond the Segre-Silberberg Pinch.

We examine theoretically the inertial migration of a neutrally buoyant rigid sphere in pressure-driven channel flow, accounting for its finite size relative to the channel width (the confinement ratio). For sufficiently large channel Reynolds numbers (Re_{c}), a small but finite confinement ratio qualitatively alters the inertial lift velocity profiles obtained using a point-particle formulation. Finite size effects lead to new equilibria, in addition to the well-known Segre-Silberberg pinch locations.

Two-fluid kinetic theory for dilute polymer solutions.

We provide a Boltzmann-type kinetic description for dilute polymer solutions based on two-fluid theory. This Boltzmann-type description uses a quasiequilibrium based relaxation mechanism to model collisions between a polymer dumbbell and a solvent molecule. The model reproduces the desired macroscopic equations for the polymer-solvent mixture.

Arterial and venous thrombotic stroke after ChAdOx1 nCoV-19 vaccine.

Vaccine-induced thrombosis with thrombocytopenia (VITT) is a recently-described condition associated with arterial and venous thrombosis following vaccination with the ChAdOx1 nCoV-19 (AstraZeneca) vaccine. This report describes two cases of stroke caused by arterial and venous thromboses presenting within 28 days of receiving the AstraZeneca vaccine. The patients were otherwise young and healthy with minimal risk factors for thrombosis yet developed a rapid, ultimately fatal neurological deterioration.

Continuous Pathway between the Elasto-Inertial and Elastic Turbulent States in Viscoelastic Channel Flow.

Viscoelastic plane Poiseuille flow is shown to become linearly unstable in the absence of inertia, in the limit of high elasticities, for ultradilute polymer solutions. While inertialess elastic instabilities have been predicted for curvilinear shear flows, this is the first ever report of a purely elastic linear instability in a rectilinear shear flow. The novel instability continues up to a Reynolds number (Re) of O(1000), corresponding to the recently identified elasto-inertial turbulent state believed to underlie the maximum-drag-reduced regime.

Early Identification, Intervention and Management of Post-stroke Spasticity: Expert Consensus Recommendations.

Stroke patients with spasticity usually require long-lasting care and interventions but frequently report that outpatient and community treatment is limited, reflecting a significant unmet need in health and social care provision. Rehabilitation and spasticity management services are essential for patient recovery, with improvements in both activity and participation reducing the burden on patients, family and society. Current clinical guidance provides scope for improvements in both post-stroke management and spasticity prevention.

Orientation Dynamics of Sedimenting Anisotropic Particles in Turbulence.

We examine the dynamics of small anisotropic particles (spheroids) sedimenting through homogeneous isotropic turbulence using direct numerical simulations and theory. The gravity-induced inertial torque acting on sub-Kolmogorov spheroids leads to pronouncedly non-Gaussian orientation distributions localized about the broadside-on (to gravity) orientation. Orientation distributions and average settling velocities are obtained over a wide range of spheroid aspect ratios, Stokes, and Froude numbers.

Evaluation of stroke thrombectomy including patients where IV thrombolysis is contraindicated or has failed: a randomized trial of two novel thrombectomy devices.

Background: Study was a PROBE design phase II randomized controlled trial (RCT). We assessed trial feasibility and technical efficacy and safety of two novel thrombectomy devices - ERIC (a retriever device) and SOFIA (a distal access catheter) - used alone or in combination depending on operator preference.

Methods: Four UK neuroscience centers enrolled adults with proximal large artery occlusion (LAO) stroke on imaging where arterial puncture was achievable within 5.

High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source.

Since the first successful serial crystallography (SX) experiment at a synchrotron radiation source, the popularity of this approach has continued to grow showing that third-generation synchrotrons can be viable alternatives to scarce X-ray free-electron laser sources. Synchrotron radiation flux may be increased ∼100 times by a moderate increase in the bandwidth ('pink beam' conditions) at some cost to data analysis complexity. Here, we report the first high-viscosity injector-based pink-beam SX experiments.

Viscoelastic Pipe Flow is Linearly Unstable.

Newtonian pipe flow is known to be linearly stable at all Reynolds numbers. We report, for the first time, a linear instability of pressure-driven pipe flow of a viscoelastic fluid, obeying the Oldroyd-B constitutive equation commonly used to model dilute polymer solutions. The instability is shown to exist at Reynolds numbers significantly lower than those at which transition to turbulence is typically observed for Newtonian pipe flow.

Enzyme intermediates captured "on the fly" by mix-and-inject serial crystallography.

Background: Ever since the first atomic structure of an enzyme was solved, the discovery of the mechanism and dynamics of reactions catalyzed by biomolecules has been the key goal for the understanding of the molecular processes that drive life on earth. Despite a large number of successful methods for trapping reaction intermediates, the direct observation of an ongoing reaction has been possible only in rare and exceptional cases.

Results: Here, we demonstrate a general method for capturing enzyme catalysis "in action" by mix-and-inject serial crystallography (MISC).

Direct Structural and Chemical Characterization of the Photolytic Intermediates of Methylcobalamin Using Time-Resolved X-ray Absorption Spectroscopy.

Cobalt-carbon bond cleavage is crucial to most natural and synthetic applications of the cobalamin class of compounds, and here we present the first direct electronic and geometric structural characteristics of intermediates formed following photoexcitation of methylcobalamin (MeCbl) using time-resolved X-ray absorption spectroscopy (XAS). We catch transients corresponding to two intermediates, in the hundreds of picoseconds and a few microseconds. Highlights of the picosecond intermediate, which is reduced in comparison to the ground state, are elongation of the upper axial Co-C bond and relaxation of the corrin ring.

Knowledge and Risk Perceptions of Occupational Infections Among Health-care Workers in Malaysia.

Health-care workers are at risk of exposure to occupational infections with subsequent risk of contracting diseases, disability, and even death. A systematic collection of occupational disease data is useful for monitoring current trends in work situations and disease exposures; however, these data are usually limited due to under-reporting. The objective of this study was to review literature related to knowledge, risk perceptions, and practices regarding occupational exposures to infectious diseases in Malaysian health-care settings, in particular regarding blood-borne infections, universal precautions, use of personal protective equipment, and clinical waste management.

Serial millisecond crystallography of membrane and soluble protein microcrystals using synchrotron radiation.

Crystal structure determination of biological macromolecules using the novel technique of serial femtosecond crystallography (SFX) is severely limited by the scarcity of X-ray free-electron laser (XFEL) sources. However, recent and future upgrades render microfocus beamlines at synchrotron-radiation sources suitable for room-temperature serial crystallography data collection also. Owing to the longer exposure times that are needed at synchrotrons, serial data collection is termed serial millisecond crystallography (SMX).

Angular correlations of photons from solution diffraction at a free-electron laser encode molecular structure.

During X-ray exposure of a molecular solution, photons scattered from the same molecule are correlated. If molecular motion is insignificant during exposure, then differences in momentum transfer between correlated photons are direct measurements of the molecular structure. In conventional small- and wide-angle solution scattering, photon correlations are ignored.

Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein.

A variety of organisms have evolved mechanisms to detect and respond to light, in which the response is mediated by protein structural changes after photon absorption. The initial step is often the photoisomerization of a conjugated chromophore. Isomerization occurs on ultrafast time scales and is substantially influenced by the chromophore environment.

Serial femtosecond X-ray diffraction of enveloped virus microcrystals.

Serial femtosecond crystallography (SFX) using X-ray free-electron lasers has produced high-resolution, room temperature, time-resolved protein structures. We report preliminary SFX of Sindbis virus, an enveloped icosahedral RNA virus with ∼700 Å diameter. Microcrystals delivered in viscous agarose medium diffracted to ∼40 Å resolution.

A novel inert crystal delivery medium for serial femtosecond crystallography.

Serial femtosecond crystallography (SFX) has opened a new era in crystallo-graphy by permitting nearly damage-free, room-temperature structure determination of challenging proteins such as membrane proteins. In SFX, femtosecond X-ray free-electron laser pulses produce diffraction snapshots from nanocrystals and microcrystals delivered in a liquid jet, which leads to high protein consumption. A slow-moving stream of agarose has been developed as a new crystal delivery medium for SFX.

Exploring the impact of workplace cyberbullying on trainee doctors.

Objectives: Workplace bullying is an occupational hazard for trainee doctors. However, little is known about their experiences of cyberbullying at work. This study examines the impact of cyberbullying among trainee doctors, and how attributions of blame for cyberbullying influence individual and work-related outcomes.

Solving protein nanocrystals by cryo-EM diffraction: multiple scattering artifacts.

The maximum thickness permissible within the single-scattering approximation for the determination of the structure of perfectly ordered protein microcrystals by transmission electron diffraction is estimated for tetragonal hen-egg lysozyme protein crystals using several approaches. Multislice simulations are performed for many diffraction conditions and beam energies to determine the validity domain of the required single-scattering approximation and hence the limit on crystal thickness. The effects of erroneous experimental structure factor amplitudes on the charge density map for lysozyme are noted and their threshold limits calculated.