Sbmyb111 act as a transcriptional activator of flavonoid synthesis in Scutellaria baicalensis.

Scutellaria baicalensis, a traditional medicinal plant originating in China, is widely cultivated for its therapeutic properties. The main bioactive substances in S. baicalensis are flavonoids, which exhibit extensive antibacterial and antiviral activities.

Multiomic Analysis Provided Insights into the Responses of Carbon Sources by Wood-Rotting Fungi .

is a newly identified species of wood-rotting fungi, with substantial aspects of its biology and ecological function yet to be clarified. A Nanopore third-generation sequencer was employed for de novo genome assembly to examine the genetic characteristics. The genome consisted of 35.

Enhancing Postharvest Quality of Fresh-Cut Changgen Mushrooms by Exogenous -Cysteine Treatment: Aspects of Accumulating Amino Acids, Triggering Energy Metabolism and Enhancing Endogenous HS Regulation.

As a rare and functional edible mushroom, the market potential of ready-to-eat fresh-cut Changgen mushrooms () is booming in developing countries. However, fresh-cut mushrooms preservation is challenging in distribution and consumption. The present study discovered that exogenous -cysteine (-Cys) treatment delayed the weight loss, browning degree, nutrition depletion and microbial contamination of fresh-cut Changgen mushrooms at 4 °C.

Dynamic changes and correlation of quality, flavor and microorganisms of Mei (Prunus mume) vinegar during fermentation and clarification.

Mei (Prunus mume) is a highly nutritious fruit whose value is often underutilized due to its perishable nature and challenges in post-maturation storage. This study evaluated the changes and correlations among quality, volatile flavor compounds (VFCs), and microorganisms in Mei vinegar (MV) during acetic acid fermentation (AAF) and low temperature clarification (LTC) using various clarifiers. The results indicated that AAF enhanced the bioactive components and antioxidant capacity of MV.

sp. alone or combined with salicylic acid inhibited spp. infection on harvested white .

Introduction: White is a popular edible mushroom. It is rich in nutrition and flavor but vulnerable to fungal disease, resulting in nutrient loss and aging.

Methods: In this study, the pathogenic fungus spp.

l-Cysteine Treatment Delayed the Quality Deterioration of Fresh-Cut Button Mushrooms by Regulating Oxygen Metabolism, Inhibiting Water Loss, and Stimulating Endogenous HS Production.

Although fresh-cut button mushrooms are popular with consumers, quality deterioration presents a significant shelf-life challenge. In this study, fresh-cut button mushrooms were treated with 0.25 g/L lcysteine (lCys) and evaluated in terms of quality, physiology, and transcriptome sequencing.

Gibberellic acid maintains postharvest quality of Agaricus bisporus mushroom by enhancing antioxidative system and hydrogen sulfide synthesis.

The application of gibberellic acid (GA ) treatment to the postharvest quality maintenance of white button mushroom (Agaricus bisporus) was investigated. The optimum concentration of exogenous GA was 100 mg/L. At this concentration, the color change was inhibited, the firmness was maintained, and the weight loss and respiratory rates were reduced.

A composite chitosan derivative nanoparticle to stabilize a W/O/W emulsion: Preparation and characterization.

For preparing stable water-in-oil-in-water emulsion, the role of nanoparticles in stabilizing the interface is very important. In this study, chitosan hydrochloride-carboxymethyl chitosan (CHC-CMC) nanoparticles were prepared considering electrostatic interactions; then the emulsion was prepared and the stability characteristics in presence of NaCl (0-200 mmol/L) and 30 d storage were studied. CHC-CMC nanoparticles (261 nm) were obtained when the CHC: CMC ratio was 1:2.

Double enzymatic hydrolysis preparation of heme from goose blood and microencapsulation to promote its stability and absorption.

Iron deficiency anemia (IDA) is the most common nutritional deficiency worldwide. This deficiency could be solved by preparing stable, edible, and absorbable iron food ingredients using environmentally friendly methods. This study investigated enzymatic hydrolysis and microencapsulation process of goose blood.

SERS optrode as a "fishing rod" to direct pre-concentrate analytes from superhydrophobic surfaces.

SERS optrodes were used to "fish" aqueous drops from superhydrophobic surfaces. The technique led to an improvement of 2-3 orders of magnitude in the lowest detectable amount of the Raman probe nile blue A, reaching 25 fg (34 attomoles). Further tests run on samples containing pesticide revealed that 20 pg of triazophos could be clearly detected from a single drop.

Fabrication of SERS swab for direct detection of trace explosives in fingerprints.

Swab sampling is of great importance in surface contamination analysis. A cotton swab (cotton Q-tip) was successfully transformed into surface-enhanced Raman scattering (SERS) substrate (SERS Q-tip) through a bottom-up strategy, where Ag NPs were first self-assembled onto the Q-tip followed by in situ growing. The capability for direct swab detection of Raman probe Nile Blue A (NBA) and a primary explosive marker 2,4-dinitrotoluene (2,4-DNT) using the SERS Q-tip was explored.

Single point calibration for semi-quantitative screening based on an internal reference in thin layer chromatography-SERS: the case of Rhodamine B in chili oil.

Thin layer chromatography (TLC) has been used in combination with surface enhanced Raman spectroscopy (SERS) for onsite screening of various analytes. In this work, we propose a novel concept for future field semi-quantitative SERS screening applications, where calibration curves were pre-built in the lab but subjected to onsite single point known standard amendment. Rhodamine B (RhB) in chili oil, a case of food scandal reported in China, was chosen as our model sample.

A silver nanoparticle embedded hydrogel as a substrate for surface contamination analysis by surface-enhanced Raman scattering.

A surface enhanced Raman scattering (SERS) substrate, capable of extracting small amounts of organic species from surfaces of different types of materials with variable roughness, has been fabricated. The substrate consists of Ag NPs encapsulated in poly(vinyl alcohol) (PVA) hydrogels, commonly known as PVA "slime". Unlike traditional SERS substrates, such as colloidal suspensions, the resulting PVA slime SERS substrate presents good viscoelasticity, allowing it to conform to the surface of various materials of arbitrary roughness.

Development of multicolor carbon nanoparticles for cell imaging.

Multicolor carbon nanoparticles (CNPs) were prepared, characterized and developed as fluorescent probes for cell imaging. The fluorescent CNPs were prepared with a facile hydrothermal oxidation route by using linear polysaccharide cellulose and cyclic oligosaccharide cyclodextrin as carbon sources. The characterizations by transmission electron microscopy show that the prepared cellulose-CNPs and cyclodextrin-CNPs are spherical, well-dispersed in water with average diameters of 100 nm and 76 nm, respectively.

Novel xylanase from a holstein cattle rumen metagenomic library and its application in xylooligosaccharide and ferulic Acid production from wheat straw.

A novel gene fragment containing a xylanase was identified from a Holstein cattle rumen metagenomic library. The novel xylanase (Xyln-SH1) belonged to the glycoside hydrolase family 10 (GH10) and exhibited a maximum of 44% identity to the glycoside hydrolase from Clostridium thermocellum ATCC 27405. Xyln-SH1 was heterologously expressed, purified, and characterized.

A protease-insensitive feruloyl esterase from China Holstein cow rumen metagenomic library: expression, characterization, and utilization in ferulic acid release from wheat straw.

A metagenomic library of China Holstein cow rumen microbes was constructed and screened for novel gene cluster. A novel feruloyl esterase (FAE) gene was identified with a length of 789 bp and encoded a protein displaying 56% identity to known esterase sequences. The gene was functionally expressed in Escherichia coli BL21 (DE3), and the total molecular weight of the recombined protein was 32.

[Quantitative structure-gas chromatographic retention relationship of polycyclic aromatic sulfur heterocycles using molecular electronegativity-distance vector].

The chemical structures of 114 polycyclic aromatic sulfur heterocycles (PASHs) have been studied by molecular electronegativity-distance vector (MEDV). The linear relationships between gas chromatographic retention index and the MEDV have been established by a multiple linear regression (MLR) model. The results of variable selection by stepwise multiple regression (SMR) and the powerful predictive abilities of the optimization model appraised by leave-one-out cross-validation showed that the optimization model with the correlation coefficient (R) of 0.

[Relationship between the laccase production of Pleurotus ostreatus and the full wavelength scan for the fermentation liquid].

Laccase, widely distributed in fungi lacking high substrate specificity, plays an important role in lignin degradation in nature and environmental protection. In order to determine or estimate the laccase production during the fermentation of liquid media, the authors studied the full-length wave scan on the rough fermentation liquid of the Pleurotus ostreatus, which produces laccase high. Combined with the normal chemical method and diameter of the laccase and mycelium stain, which grew on the PDA (potato dextrose agar) plate with guaiacol added, we could get the exact information of laccase production.