Probing the Demulsification Mechanism of Emulsion with SPAN Series Based on the Effect of Solid Phase Particles.

The solid particles in the produced fluids from the oil wells treated by compound flooding can greatly stabilize the strength of the interfacial film and enhance the stability of the emulsion, increasing the difficulty of processing these produced fluids on the ground. In this paper, the oil phase and the water phase were separated from the SPAN series emulsions by electrical dehydration technology and adding demulsifier agents. The changing trends of the current at both ends of the electrodes were recorded during the process.

The impact of social support on the quality of life among older adults in China: An empirical study based on the 2020 CFPS.

Background: As aging issues become serious, how to guarantee and improve the quality of life among older adults has become a hot topic in China. This article is aimed to discuss the impact of formal and informal social support on the quality of life among older adults and the differences in gender and urban-rural areas.

Methods: The data used in this article are from the 2020 China Family Panel Studies (CFPS).

Empathy in Chinese eight-year medical program students: differences by school year, educational stage, and future career preference.

Backgroud: To examine the psychometric properties of a Chinese translation of the Jefferson Scale of Empathy (Student-version, JSE-S), and to study differences in empathy scores among eight-year undergraduate medical students across gender, year of study, and future career preference.

Methods: The JSE-S was administered to 442 participants from December 2016 to July 2017, who were all first- to seventh-year students on an eight-year medical education course at Fudan University. Factor analysis was used to examine the underlying components of the Chinese version of the JSE-S.

Prednisone may induce immunologic tolerance by activating the functions of decidual immune cells in early pregnancy.

The objective of this study was to investigate alterations in human first-trimester decidual immune cells (DICs) and relevant cytokines after treatment with prednisone. Decidual lymphocytes were treated with prednisone alone, cytokines alone or the combination of prednisone and cytokines. Levels of STAT3, STAT5, RORC and FOXP3 mRNA were assayed using quantitative real-time PCR, proportions of CD4 T helper 17 (Th17) and CD4 T regulatory (Treg) cells were measured using flow cytometry, and concentrations of interleukin (IL)-17A and IL-10 were determined using enzyme-linked immunosorbent assay.

Effects of electroacupuncture on uterine morphology and expression of oestrogen receptors in ovariectomised rats.

Aim: To observe the effects of electroacupuncture (EA) on uterine morphology and expression of oestrogen receptor (ER) α and β in ovariectomised (OVX) rats.

Methods: Thirty female Sprague-Dawley rats with regular 4-day oestrus cycles were divided into a sham operation group (Control, n=10) and two OVX groups that remained untreated (OVX group, n=10) or received EA treatment (OVX+EA group, n=10). In the latter group, EA was applied at CV4, CV3, SP6 and bilateral (30 min per day) for 3 days.

Role of the medial preoptic area in electroacupuncture treatment of reproductive dysfunction in ovariectomised rats.

Objective: To investigate the role of the medial preoptic area (MPOA) of the hypothalamus in electroacupuncture (EA) treatment of reproductive dysfunction in ovariectomised (OVX) rats and to examine the underlying mechanisms of action.

Methods: 62 female Sprague-Dawley rats (200-220 g) underwent bilateral ovariectomy (OVX). Three weeks later, 32 rats assigned to experiment 1 were randomly divided into four groups: two groups underwent MPOA lesioning (n=16) followed by EA at CV4, CV3, SP6 and bilateral Zigong (30 min per day) for 3 days (OVX+MPOA+EA group, n=8) or no treatment (OVX+MPOA group, n=8); and two groups underwent sham (S) surgery (n=16) followed by EA (OVX+SMPOA+EA group, n=8) or no treatment (OVX+SMPOA group, n=8).

Targeting Cellular Metabolism Chemosensitizes the Doxorubicin-Resistant Human Breast Adenocarcinoma Cells.

Metabolic energy preferentially produced by glycolysis was an advantageous metabolic phenotype of cancer cells. It is also an essential contributor to the progression of multidrug resistance in cancer cells. By developing human breast cancer MCF-7 cells resistant to doxorubicin (DOX) (MCF-7/MDR cells), the effects and mechanisms of 2-deoxy-D-glucose (2DG), a glucose analogue, on reversing multidrug resistance were investigated.

The actin-polymerizing activity of SipA is not essential for Salmonella enterica serovar Typhimurium-induced mucosal inflammation.

Salmonella enterica serovar Typhimurium depends on type III secretion systems to inject effector proteins into host cells to promote bacterial invasion and to induce intestinal inflammation. SipA, a type III effector, is known to play important roles in both the invasion and the elicitation of intestinal inflammation. The actin-modulating activity of SipA has been shown to promote Salmonella entry into epithelial cells.

Involvement of TIP60 acetyltransferase in intracellular Salmonella replication.

Background: Salmonella enterica is a facultative intracellular pathogen that replicates within a membrane-bound compartment termed Salmonella containing vacuole (SCV). The biogenesis of SCV requires Salmonella type III protein secretion/translocation system and their effector proteins which are translocated into host cells to exploit the vesicle trafficking pathways. SseF is one of these effectors required for SCV formation and Intracellular Salmonella replication through unknown mechanisms.

[Adipogenic potential of bone marrow mesenchymal stem cells derived from ovariectomized rats].

In order to study the effect of ovariectomy on the capacity of rat MSCs' differentiation into adipocytes, we established the animal models of osteoporosis by performing ovariectomy on the 3-months-old and 6-month-old female SD rats. Then MSCs were isolated from these rats by means of the density-gradient centrifugation method. After adipogenic induction (ADI), lipid droplets were detected by oil red O staining, and the mRNA level of lipoprotein lipase(LPL) was measured by RT-PCR assay.

[A potential role for the bone marrow mesenchymal stem cell in the pathogenesis of osteoporosis by ovariectomy in rat].

The purpose of this paper is to investigate the osteogenesis and adipogenesis in bone marrow mesenchymal stem cells (MSCs) isolated from normal rats and osteoporotic rats by ovariectomy. Osteoporotic animal model was established in 3 month-old and 6 month-old female Sprague-Dawley (SD) rats by ovariectomy. Animal experiments were divided into 4 groups: 1) control-3 group; 2) ovx-3 group; 3) control-6 group and 4) ovx-6 group.

[Osteogenic potential of bone marrow mesenchymal stem cells from ovariectomied osteoporotic rat].

Objective: To investigate the difference of osteogenic potential of bone marrow mesenchymal stem cells (MSCs) between healthy rats and osteoporotic rats.

Methods: We established the animal model of osteoporosis by performing ovariectom on the 3-month-old female Sprague-Dawley rats. Bone marrow mesenchymal stem cells(MSCs) were isolated from the rats of control group and of ovariectomized (ovx) group by means of the density-gradient centrifugation method, and the 3rd-4th passage MSCs were used in all the experiments.