Target-oriented synthesis of yellow fluorescent nitrogen-doped carbon dots for intelligent and real-time visual detection of malachite green in fish.

As a triphenylmethane dye, malachite green (MG) is strictly regulated in aquaculture by numerous countries due to its persistence and acute toxicity, posing significant risks to human health. This has driven the demand for rapid and sensitive detection methods for MG. Herein, we present a lateral flow test strips (LFTS)-based sensing platform utilizing yellow fluorescent nitrogen-doped carbon dots (N-CDs) for real-time visual detection of MG.

Specific dual-mode fluorometric and colorimetric detection of nitrite based on oxidase-like activity of carbon dots/iron alkoxide composite.

Trace detection of nitrite in food has gained increasing attention due to its connection with biological and environmental processes. Herein, a dual-mode fluorometric/colorimetric sensor is established for nitrite detection by combining the oxidase-like activity of iron alkoxide (IA) with the bimodal excitation properties of nitrogen-doped carbon dots (N-CDs). IA oxidizes the colorless 3,3',5,5'-tetramethylbenzidine into blue-colored 3,3',5,5'-tetramethylbenzidine oxide.

De novo transcriptome sequencing of Isaria cateniannulata and comparative analysis of gene expression in response to heat and cold stresses.

Isaria cateniannulata is a very important and virulent entomopathogenic fungus that infects many insect pest species. Although I. cateniannulata is commonly exposed to extreme environmental temperature conditions, little is known about its molecular response mechanism to temperature stress.