Development of multifunctional BP@MoS two-dimensional hybrid nanomaterials for label-free surface-enhanced Raman spectroscopy detection and synergistic light-conversion depredation of pathogenic bacteria.

Infectious diseases caused by pathogenic bacteria pose a significant challenge to global public health, underscoring the urgent need for early detection and on-site sterilization technology. In this study, we proposed a multifunctional two-dimensional BP@MoS nanocomposites, which not only enabled sensitive bacterial detection but also demonstrated efficient sterilization capabilities. Specially, the unique flower-like surface microstructure of the nanocomposites promoted target enrichment efficiency, while its hybridized band structure facilitated powerful charge transfer, thereby facilitated a label-free surface-enhanced Raman spectroscopy (SERS)-based assay of Escherichia coli (E.

Dual-Mode SERS Lateral Flow Aptamer Assay with Machine Learning-Driven Highly Sensitive Interferon-γ Detection.

Interferon-γ (IFN-γ), a key pro-inflammatory cytokine, is widely recognized as a critical biomarker for diagnosing and monitoring various immune-related conditions. However, its typically low concentrations in biological fluids─at the picogram-per-milliliter (pg/mL) level─necessitate ultrasensitive detection strategies for early clinical intervention. Here, we report a dual-mode surface-enhanced Raman scattering (SERS) lateral flow aptamer assay that employs a competitive binding mechanism between IFN-γ and its complementary DNA for aptamer recognition.

Label-free SERS detection of foodborne pathogens based on a flexible PMMA-BP@MoS binary substrate.

Foodborne pathogenic bacteria, acting as direct or indirect contaminants of food and water sources, have emerged as a significant global food safety issue. Thus, the advancement of rapid, sensitive, and precise methods for detecting foodborne pathogens is of paramount importance for effective food safety monitoring. In response to this critical need, we have successfully prepared a flexible PMMA-BP@MoS-based surface-enhanced Raman scattering (SERS) substrate through a two-step strategy involving hydrothermal synthesis and spin coating.

Advances in CRISPR/Cas13a-based biosensors for non-coding RNA detection.

Non-coding RNAs play crucial roles in disease initiation and progression, making them promising biomarkers for early diagnosis and treatment monitoring. Conventional nucleic acid diagnostic methods, including polymerase chain reaction (PCR), next-generation sequencing (NGS), and enzyme-linked immunosorbent assay (ELISA), alongside emerging techniques such as single-molecule fluorescence in situ hybridization (smFISH), nanopore sequencing, and single-cell RNA sequencing (scRNA-seq), face inherent limitations in detecting regulatory non-coding RNAs. These challenges include laborious workflows, prolonged processing times, and technical complexities, hindering their broad applicability in rapid and high-throughput RNA analysis.

Exploration of Epigenetic Mechanisms and Biomarkers Among Patients with Very-Late-Onset Schizophrenia-Like Psychosis.

Objective: This study aimed to identify DNA methylation patterns associated with Very Late-Onset Schizophrenia-like Psychosis (VLOSLP) and to develop methylation-based biomarkers that differentiate VLOSLP from Schizophrenia (SCZ) and Alzheimer's Disease (AD).

Methods: We analyzed methylation microarray datasets (n = 1218) from SCZ and AD patients obtained from the GEO database. We then collected blood samples from VLOSLP patients and age-matched healthy controls (n = 80) at the Wuxi Mental Health Center for methylation microarray profiling and bisulfite sequencing validation.

Voxel-based alterations in spontaneous brain activity among very-late-onset schizophrenia-like psychosis: A preliminary resting-state functional magnetic resonance imaging study.

Background: Very late-onset schizophrenia-like psychosis (VLOSLP) is a subtype of schizophrenia spectrum disorders in which individuals experience psychotic symptoms for the first time after the age of 60. The incidence of VLOSLP shows a linear relationship with increasing age. However, no studies have reported alterations in spontaneous brain activity among VLOSLP patients and their correlation with cognitive function and clinical symptoms.

Flexible chip-based wearable sensors enhanced by gold nanoparticles for IFN-γ detection.

Cytokines, essential regulatory proteins orchestrating various physiological processes and immune responses, play a pivotal role in assessing health status. Conventional cytokine detection methods, such as enzyme-linked immunosorbent assays, are reliable but time-consuming. Electrochemical aptamer-based sensors offer rapid response and higher selectivity, but fall short of achieving non-invasive detection.

Disparities in Utilization of Immune Checkpoint Inhibitor Therapy Among Older Patients With Advanced Non-Small Cell Lung Cancer: A SEER-Medicare Analysis.

Purpose: In the United States, there are disparities in access to care for patients with non-small cell lung cancer (NSCLC) on the basis of socioeconomic and racial/ethnic factors. This study investigates the association between race/ethnicity and the utilization of immune checkpoint inhibitor (ICI) therapy among older patients with advanced NSCLC (aNSCLC).

Methods: This retrospective study used data from the SEER-Medicare-linked database.

Rethinking how mobile units can catalyze progress on lung cancer screening: a scoping review of what we have learned.

Background: Despite United States Preventive Services Task Force (USPSTF) recommendations, low uptake of lung cancer screening (LCS) highlights the need for measures to promote adoption. This scoping review aims to outline the global landscape of mobile low-dose computed tomography (LDCT) platforms, summarizing research and evaluating efficacy in screening at-risk populations.

Methods: We comprehensively searched Cumulative Index to Nursing and Allied Health Literature (CINAHL), PubMed, Embase, Scopus, and Web of Science for articles published between 2017 and 2023.

Heat transfer processes in 'Shine Muscat' grapevine leaves in solar greenhouses under different irrigation treatments.

The use of solar greenhouses in China is increasing because they permit environmental conditions to be controlled. Studies of the heat transfer processes in the leaves of plants cultivated within solar greenhouses are needed. Here, we studied heat transfer processes in 'Shine Muscat' grapevine leaves under moderate deficit irrigation (MDI), severe deficit irrigation (SDI), and full irrigation (FI) treatments under varying weather conditions.

RNA reporter based CRISPR/Cas12a biosensing platform for sensitive detection of circulating tumor DNA.

CRISPR/Cas biotechnology provides an exceptional platform for biosensor development. To date, the reported CRISPR/Cas biosensing systems have shown extraordinary performance for nucleic acids, small molecules, small proteins and microorganism detection. The CRISPR/Cas12a biosensing system, as a typical example, has been well established and applied for both nucleic acids and non-nucleic acids target detection.

Paper based SERS aptasensor towards dual-modal detection of interferon gamma.

Interferon gamma (IFN-γ), can serve as an active diagnostic biomarker of a broad spectrum of diseases such as auto inflammatory disease, viral and bacterial, parasites infections, and tumor control. The low physiological concentration of IFN-γ at pg‧mL level for most diseases such as tuberculosis and lung cancer demand highly sensitive and selective detection methods. To achieve the goal, a novel paper-based SERS aptasensor towards rapid, dual-modal (visual and ultrasensitive) detection of IFN-γ is presented for the first time.

Allostatic load and risk of all-cause, cancer-specific, and cardiovascular mortality in older cancer survivors: an analysis of the National Health and Nutrition Examination Survey 1999-2010.

Background: Allostatic load has been linked to an increased risk of death in various populations. However, to date, there is no research specifically investigating the effect of allostatic load on mortality in older cancer survivors.

Aims: To investigate the association between allostatic load (AL) and mortality in older cancer survivors.

Survival Differences by Comorbidity Burden among Patients with Stage I/II Non-Small-Cell Lung Cancer after Thoracoscopic Resection.

We sought to compare overall survival (OS) by comorbidity burden among patients with stage I/II non-small cell lung cancer (NSCLC) who received thoracoscopic resection. Utilizing data from the National Cancer Database, we conducted a survival analysis among patients aged 50+ with stage I/II NSCLC who received thoracoscopic resection between 2010 and 2017. The comorbidity burden was measured by the Charlson comorbidity index (CCI, 0, 1, 2+).

Associations of Total Body Fat Mass and Skeletal Muscle Index with All-Cause and Cancer-Specific Mortality in Cancer Survivors.

: The importance of body composition on cancer outcomes is of great clinical interest. Measures of body composition that differentiate fat mass from skeletal muscle mass can help redefine our understanding of body composition for cancer survival. We investigated whether the risk of all-cause and cancer-specific mortality differ by levels of total fat mass and sarcopenia status in cancer survivors.

The Intersectionality between Race, Ethnicity, and Residential-Level Socioeconomic Status in Disparities of Head and Neck Cancer Outcomes: A SEER Study.

Background: Head and neck cancer (HNC) mortality differs by race, ethnicity, and socioeconomic status (SES). However, it is unclear whether the relationship between race/ethnicity and HNC-specific mortality varies according to the residence-level SES.

Methods: Data from the Surveillance Epidemiology and End Results database included participants with primary HNC between 2006 and 2017 (followed through 2018) to assess the joint association of race/ethnicity and census-tract level SES Yost-index groups (quintiles) with all-cause and HNC-specific mortalities.

The antifungal potential of the chelating agent EDTA against postharvest plant pathogen Botrytis cinerea.

Botrytis cinerea is a phytopathogenic fungus that causes gray mold, a major postharvest disease of fruits and vegetables. Chemical fungicides remain the main solution to control Botrytis disease, but concerns have raised about their safety to environment and human health, and there is an increasing need for development of more effective and less toxic treatments. In this study the divalent cation chelating agent ethylenediaminetetraacetic acid (EDTA) exhibited marked antifungal activity against B.

Low muscle mass is associated with a higher risk of all-cause and cardiovascular disease-specific mortality in cancer survivors.

Objectives: Individuals with prior cancer diagnosis are more likely to have low muscle mass (LMM) than their cancer-free counterparts. Understanding the effects of LMM on the prognosis of cancer survivors can be clinically important. The aim of this study was to investigate whether risks for all-cause and cardiovascular disease (CVD)-specific mortality differ by status of LMM in cancer survivors and a matched cohort without cancer history.

Perceived Severity of COVID-19 in a Longitudinal Study in Detroit, Michigan.

Objective: To slow down the spread of SARS-CoV-2, many countries have instituted preventive approaches (masks, social distancing) as well as the distribution of vaccines. Adherence to these preventive measures is crucial to the success of controlling the pandemic but decreased perceptions of disease severity could limit adherence. The aim of our study was to observe changes in perceived personal severity and perceived community severity; the study also explored their predictors.

Development of Recombinase-Aided Amplification (RAA)-Exo-Probe and RAA-CRISPR/Cas12a Assays for Rapid Detection of in Food Samples.

is the major cause of campylobacteriosis, one of the most common foodborne illnesses worldwide. Here, we report the development of RAA-exo-probe and RAA-CRIPSR/Cas12a assays for the detection of in food samples. The two assays were found to be highly specific to and highly sensitive, as they were one log more sensitive compared to the traditional culture method, with detection thresholds of 9 and 5 copies per reaction, respectively.

Disposable Electrochemical Aptasensor Based on Graphene Oxide-DNA Complex as Signal Amplifier towards Ultrasensitive Detection of Ochratoxin A.

Signal amplification is crucial in developing a reliable disposable screen-printed carbon electrodes (SPCEs)-based biosensor for analyte detection with a narrow detection window. This work demonstrated a novel label-free electrochemical aptasensor based on SPCEs for the ultrasensitive detection of ochratoxin A (OTA). The graphene oxide-DNA (GO-DNA) complex as a signal amplifier with easy preparation was investigated for the first time.

Advanced detection and sensing strategies of Pseudomonas aeruginosa and quorum sensing biomarkers: A review.

Pseudomonas aeruginosa (P. aeruginosa), a ubiquitous opportunistic pathogen, can frequently cause chronic obstructive pulmonary disease, cystic fibrosis and chronic wounds, and potentially lead to severe morbidity and mortality. Timely and adequate treatment of nosocomial infection in clinic depends on rapid detection and accurate identification of P.

Label-Free and Highly-Sensitive Detection of Ochratoxin A Using One-Pot Synthesized Reduced Graphene Oxide/Gold Nanoparticles-Based Impedimetric Aptasensor.

Ochratoxin A (OTA) primarily obtained by the genera and , is one of the toxic substances for different organs and systems of the human body such as the kidney, liver, neurons and the immune system. Moreover, it is considered to cause tumors and fetal malformation even at a very low concentration. Fast and sensitive assay for detection of OTA at ultralow levels in foods and agricultural products has been an increasing demand.