Comparative pan-genome analysis of Huperzia and Phlegmariurus and transcriptomics reveals thermal adaptation in Huperzia.

Huperzia and Phlegmariurus are ancient genera within the Lycopodiaceae family with significant medicinal value and ecological adaptability, yet the evolutionary dynamics and genetic diversity of their chloroplast genomes remain poorly characterized. Specifically, critical aspects such as intergeneric differences, phylogenetic relationships, and adaptive evolution within their chloroplast genomes remain insufficiently explored. This study analyzed the chloroplast genomes of 66 species from these two genera through comparative genomics to elucidate their structural dynamics and adaptive mechanisms.

Implications of the different submerged extent of typical sandbars to local DOM and SDOM during the pre-flood season in the middle reaches of Yangtze River.

In large rivers, sandbar evolution is mainly driven by changes in discharge, flow velocity, and sediment concentration in the water, which can shape the implications of local dissolved organic matter (DOM) and sediment dissolved organic matter (SDOM). After the completion of the Three Gorges Dam (TGD), the water level in the middle reaches of the Yangtze River changes rapidly during the year, and the submerged extent of the Yangtze River sandbars are significantly affected by the change in water level, so that when the water level rises to the defense level or higher, the sandbars may be completely submerged. As a result, different submerged extent such as periodically and long-term have occurred on the typical sandbars.