Screen of FDA-approved drug library identifies vitamin K as anti-ferroptotic drug for osteoarthritis therapy through Gas6.

Ferroptosis of chondrocytes is a significant contributor to osteoarthritis (OA), for which there is still a lack of safe and effective therapeutic drugs targeting ferroptosis. Here, we screen for anti-ferroptotic drugs in Food and Drug Administration (FDA)-approved drug library a high-throughput manner in chondrocytes. We identified a group of FDA-approved anti-ferroptotic drugs, among which vitamin K showed the most powerful protective effect.

PSMD14 Stabilizes SLC7A11 to Ameliorate Glucocorticoid-Induced Osteoporosis by Suppressing Osteocyte Ferroptosis.

Glucocorticoid-induced osteoporosis (GIOP) remains the most prevalent complication compromising bone health in patients undergoing glucocorticoid (GC) therapy. Despite its clinical significance, osteocyte death, a pivotal initiator of GC-driven bone metabolic imbalance, has received insufficient attention. This study identifies ferroptosis, an iron-dependent regulated cell death mechanism, as a novel pathological phenotype of osteocytes in GC microenvironments.

Daphnetin-mediated mitophagy alleviates intervertebral disc degeneration via the Nrf2/PINK1 pathway.

Intervertebral disc degeneration (IDD) is a major cause of low back pain (LBP), and effective therapies are still lacking. Reactive oxygen species (ROS) stress induces NLRP3 inflammasome activation, and this, along with extracellular matrix metabolism (ECM) degradation in nucleus pulposus cells (NPCs), plays a crucial role in the progression of IDD. Daphnetin (DAP) is a biologically active phytochemical extracted from plants of the , which possesses various bioactivities, including antioxidant properties.

Orientin promotes diabetic wounds healing by suppressing ferroptosis via activation of the Nrf2/GPX4 pathway.

Diabetic patients often experience delayed wound healing due to impaired functioning of human umbilical vein endothelial cells (HUVECs) under high glucose (HG) conditions. This is because HG conditions trigger uncontrolled lipid peroxidation, leading to iron-dependent ferroptosis, which is caused by glucolipotoxicity. However, natural flavonoid compound Orientin (Ori) possesses anti-inflammatory bioactive properties and is a promising treatment for a range of diseases.

Gamma-oryzanol alleviates osteoarthritis development by targeting Keap1-Nrf2 binding to interfere with chondrocyte ferroptosis.

Osteoarthritis (OA) is a prevalent joint disorder pathologically correlated to chondrocyte ferroptosis. Gamma-oryzanol (γ-Ory), as a first-line drug for autonomic disorders, aroused our interest because of its antioxidant, lipid-lowering, and hypoglycemic potential. The purpose of this study was to investigate the potential impact and mechanism of γ-Ory in treating OA.

ZnVO/NC hybrid microspheres self-assembled by layered porous nanosheets as a superior anode material for lithium/sodium-ion batteries.

Zinc-vanadium oxides have been attracting increasing consideration as anode materials for lithium/sodium-ion batteries (LIBs and SIBs) recently. Present applications are hampered by issues, including their inferior electric conductivity and enormous volume variation. Herein, nitrogen-doped carbon wrapped ZnVO (ZnVO/NC) microspheres composed of abundant nanosheets were developed as an anode material by a self-assembly strategy and subsequent surface decoration.

Hierarchical NiCoSe nanoneedles/nanosheets with N-doped 3D porous graphene architecture as free-standing anode for superior sodium ion batteries.

In order to cope with the problem of insufficient lithium metal reserves, sodium ion batteries (SIBs) are proposed and extensively studied for the next-generation batteries. In our work, hierarchical NiCoSe nanoneedles/nanosheets are deposited on the skeleton of N-doped three dimensional porous graphene (NPG) by a convenient solvothermal method and subsequent gas-phase selenization process. Compared with NiCoSe powder, the optimized NiCoSe/N-doped porous graphene composite (denoted as NCS@NPG) as self-supporting anode exhibits the excellent electrode activity for SIBs, with a specific capacity of 500 mAh/g and 257 mAh/g at a current density of 0.

Controlled synthesis of N-doped carbon and TiO double-shelled nanospheres with encapsulated multi-layered MoO nanosheets as an anode for reversible lithium storage.

α-Phase molybdenum trioxide (α-MoO) is one of the promising anode materials for lithium storage due to its high theoretical capacity and unique intercalation reaction mechanism. Herein, through an efficient step-by-step solvothermal synthesis strategy, multi-layered MoO nanosheets are encapsulated by nitrogen-doped carbon (NC) and ultrathin TiO double-shells to obtain hierarchical core-shell nanospheres (MoO@TiO@NC). The unique nanostructure enables shortening the Li diffusion distance, buffer the volume change during the intercalation/deintercalation process, and increase the active sites for the electrochemical reaction.

Highly uniform nitrogen-doped carbon decorated MoO nanopopcorns as anode for high-performance lithium/sodium-ion storage.

Molybdenum dioxides (MoO) featuring low cost and high theoretical capacity endow them competitive anode materials for lithium-ion batteries (LIBs)/sodium-ion batteries (SIBs). However, the low electrical conductivity and severe volume expansion occurring during the ion insertion/extraction process hamper their practical application. Herein, a novel dual-annealing design has been developed for the synthesis of highly uniform MoO nanopopcorns decorated with nitrogen-doped carbon shell (MoO/NC).

[Preparation, characterization and potential application of monoclonal antibody 18A4 against AGR2].

Aim: To prepare mouse monoclonal antibodies (mAb) against AGR2 (human homolog of xenopus anterior gradient 2), to characterize these antibodies' properties, and to develop potential applications.

Methods: BALB/c mice were immunized with AGR2-MBP (maltose binding protein) fusion protein. The mAb was prepared by hybridoma technique and purified by protein G affinity chromatography.