Engineered Salmonella Enteritidis vector targeting innate immune molecules provides protection against Salmonella Enteritidis and Salmonella Typhimurium.

Targeting the innate immune response is a critical factor in vaccine success. There are numerous serotypes of Salmonella, and cross-reactivity among different serotypes or even strains within the same serotype is limited. This makes it difficult for vaccines to provide broad protection, posing a challenge to effective prevention and control.

Construction and protective evaluation of a recombinant attenuated vaccine delivering antigens.

() is a highly detrimental avian pathogen that has resulted in substantial economic losses within the global poultry industry. The existing vaccines, presently in use, fail to offer comprehensive protection. The objective of this study was to create recombinant attenuated vaccine (RASV) strains, namely rSC0130(pS-rRS01790), rSC0130(pS-rBMP), rSC0130(pS-rGrpE), rSC0130(pS-rRS00900), and rSC0130(pS-rRS00275), expressing RS01790, BMP, GrpE, RS00900, and RS00275 proteins, and verify their protective effects.

Identification of the governing equation of stimulus-response data for run-and-tumble dynamics.

The run-and-tumble behavior is a simple yet powerful mechanism that enables microorganisms to efficiently navigate and adapt to their environment. These organisms run and tumble alternately, with transition rates modulated by intracellular chemical concentration. We introduce a neural network-based model capable of identifying the governing equations underlying run-and-tumble dynamics.

Signal peptides enhance immunogenicity and protection of recombinant Salmonella enterica serovar Choleraesuis vectors against Streptococcus suis.

Streptococcus suis (S. suis) is prevalent worldwide, threatening the pig farming industry and public health. At present, there is no effective vaccine.

Heterologous signal peptide grafting enhances the immune efficacy of Salmonella vectors delivering hemagglutinin against H7N9 avian influenza virus.

Salmonella enterica serovar Typhimurium (S. Typhimurium) vectors, which induce broad cellular and humoral immune responses, are excellent candidates for delivering foreign antigens. However, S.

Recombinant Salmonella vector delivery 3M2e-ferritin fusion nanoparticles provide cross protection against H9N2 and H7N9 avian influenza viruses.

In recent years, the advancement of nanomedicine has revolutionized the field of vaccine development. Numerous nanoparticle-based nano vaccine candidates have been continuously developed. Herein, we describe a universal avian influenza virus (AIV) vaccine candidate based on ferritin self-assembling nanoparticles (NPs).

Biased Lévy Walk Enables Light Gradient Sensing in Euglena gracilis.

We examine the navigation behavior of the photosensitive alga Euglena gracilis in confined environments. Under uniform lighting conditions, E. gracilis exhibits stochastic movements with nearly straight runs interrupted by abrupt directional changes.

Real-time, non-destructive monitoring of the aggregation behavior of silver nanoparticles using nano-impact electrochemistry.

Silver nanoparticles (AgNPs) are widely used in daily life, with their aggregation behavior greatly impacting their application potential. Thus, studying the aggregation of AgNPs is crucial for their practical applications. Nano-impact electrochemistry (NIE) has gained significant attention due to its rapid, cost-effective, and analysis capabilities at the single-nanoparticle level.

Weakened fungus-assisted remediation of Cd(II) after addition of fibrous palygorskite.

Aims: Rhodotorula mucilaginosa (Rho) has a strong ability to absorb heavy metals. In this study, the influences of fibrous palygorskite (Pal) on fungus-assisted remediation of Cd2+ was investigated.

Methods And Results: Three sets of treatments were performed, i.

A Crystalline Polymer Cathode for Sodium-Organic Batteries.

Sodium-organic batteries (SOBs) are widely recognized as a rising star in large-scale energy storage technology because of their abundant resources, high energy density, and environmental friendliness. However, the poor cyclability and rate performance due to high solubility and poor conductivity with organic electrode materials limit their practical applications. Here, we constructed a new crystalline polymer, poly(tetraaminobenzoquione) (PTABQ), featuring a layered structure with a conjugated backbone and intermolecular hydrogen bonds.

Effect of Tafamidis on Clinical and Functional Parameters in Transthyretin Amyloid Cardiomyopathy.

Background: Transthyretin amyloid cardiomyopathy (ATTR-CM) has recently gained recognition as a rare and fatal disease. Tafamidis, a first-in-class transthyretin stabilizer, has emerged as a promising agent for attenuating disease progression. Nevertheless, how tafamidis alters clinical and imaging parameters remains unclear.

Left atrial reservoir strain as a surrogate marker for atrial fibrillation burden in patients with non-valvular atrial fibrillation.

Background: To evaluate the correlation between atrial fibrillation (AF) burden and two-dimensional LA (left atrium) longitudinal reservoir strain (LARS) to explore the potential clinical utility of this novel parameter in patients with AF.

Methods: This cross-sectional study was conducted at the cardiac electrophysiology clinic of a tertiary centre by consecutively enrolling patients with non-valvular AF. The AF burden was evaluated using 14-day patch-based electrocardiography and defined as the percentage of time spent in AF during a 14-day monitoring period.

Fluidized Electrochemical Exfoliation of Layered Transition Metal Dichalcogenides toward Fast Production of High-Quality Nanosheets in the Aqueous Phase.

The transformation of bulk transition-metal dichalcogenide (TMD) particles into ultrathin nanosheets with both an acceptable yield and preserved crystalline integrity presents a substantial challenge in electrochemical exfoliation. This challenge arises from the continuous potential stress that the materials experience in traditional exfoliation setups. Herein, we propose a new fluidized electrochemical exfoliation (FEE) method to efficiently transform TMD powders into high-quality, few-layered TMD nanosheets in the aqueous phase.

Impact of Tafamidis on [Tc]Tc-pyrophosphate Scintigraphy in Ala97Ser Hereditary Transthyretin amyloid cardiomyopathy: significant initial reduction with stable Long-Term effects.

Objective: Tafamidis has shown potential in slowing disease progression in patients with transthyretin amyloid cardiomyopathy (ATTR-CM). This study aimed to evaluate serial changes on [Tc]Tc-pyrophosphate (PYP) scintigraphy during tafamidis treatment for hereditary ATTR-CM.

Methods: We retrospectively analyzed a prospectively collected cohort of Ala97Ser (A97S) hereditary ATTR-CM patients treated with tafamidis (61 mg/day) and a control group comprising A97S hereditary ATTR-CM patients who had not received disease-modifying medications.

A universal live vaccine platform against multiple serotypes Streptococcus suis based on polyvalent antigen protein.

Streptococcus suis (S. suis) is a major pathogen that poses a long-term threat to swine populations. Due to its foodborne transmission, this pathogen has recently emerged as a leading cause of meningitis in humans, presenting a significant public health challenge.

A Purely Biomanufactured System for Delivering Nanoparticles and STING Agonists.

Nanovaccines have significantly contributed in the prevention and treatment of diseases. However, most of these technologies rely on chemical or hybrid semibiological synthesis methods, which limit the manufacturing performance advantages and improved inoculation outcomes compared with traditional vaccines. Herein, a universal and purely biological nanovaccine system is reported.

Enhancement of protective efficacy of recombinant attenuated Salmonella typhimurium delivering H9N2 avian influenza virus hemagglutinins(HA) antigen vaccine candidate strains by C-C motif chemokine ligand 5 in chickens(chCCL5).

The H9N2 inactivated avian influenza vaccine cannot induce cellular and mucosal immune responses, while the attenuated Salmonella vector as an intracellular bacterium can induce dominant cellular and mucosal immune responses. However, it provides low protection against the virus when delivering viral antigens and needs further optimization. Chicken C-C motif chemokine ligand 5 (chCCL5) is an important CC chemokine associated with immune cell chemotaxis, migration, and viral infection.

Apatinib combined with temozolomide in diffuse midline glioma: a novel and effective therapy.

Purpose: Diffuse midline glioma (DMG), H3 K27M-mutant is a type of diffuse high-grade glioma that occurs in the brain midline carrying an extremely poor prognosis under the best efforts of surgery, radiation, and other therapies. For better therapy, we explored the efficacy and toxicity of a novel therapy that combines apatinib and temozolomide in DMG.

Methods: A retrospective analysis of 32 patients with DMG who underwent apatinib plus temozolomide treatment was performed.

Divergent Synthesis of Enantioenriched Silicon-Stereogenic Benzyl-, Vinyl- and Borylsilanes via Asymmetric Aryl to Alkyl 1,5-Palladium Migration.

Functionalization of Si-bound methyl group provides an efficient access to diverse organosilanes. However, the asymmetric construction of silicon-stereogenic architectures by functionalization of Si-bound methyl group has not yet been described despite recent significant progress in producing chiral silicon. Herein, we disclosed the enantioselective silylmethyl functionalization involving the aryl to alkyl 1,5-palladium migration to access diverse naphthalenes possessing an enantioenriched stereogenic silicon center, which are inaccessible before.

Evaluation of immunogenicity and protective efficacy of outer membrane vesicles from Salmonella Typhimurium and Salmonella Choleraesuis.

Outer membrane vesicles (OMVs) are membranous structures frequently observed in Gram-negative bacteria that contain bioactive substances. These vesicles are rich in bacterial antigens that can activate the host's immune system, making them a promising candidate vaccine to prevent and manage bacterial infections. The aim of this study was to assess the immunogenicity and protective efficacy of OMVs derived from Salmonella enterica serovar Typhimurium and S.

The mechanistic view of non-coding RNAs as a regulator of inflammatory pathogenesis of Parkinson's disease.

Parkinson's disease (PD) is a neurodegenerative disorder characterized by the progressive loss of dopaminergic neurons in the substantia nigra pars compacta, leading to motor and non-motor symptoms. Emerging evidence suggests that inflammation plays a crucial role in the pathogenesis of PD, with the NLRP3 inflammasome implicated as a key mediator. Nfon-coding RNAs (ncRNAs), including microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), have recently garnered attention for their regulatory roles in various biological processes, including inflammation.