Isolation and Genetic Characterization of Three Palyam Serogroup Orbiviruses From Spp. and Associated Infections in Cattle in Yunnan, China.

Palyam serogroup virus (PALV) is a potential pathogen prevalent in Asia, Australia, and Africa that may cause abortion and teratogenesis in ruminants, especially cattle. In this study, we isolated three PALV strains (MY_C17, ML_C40, and SZ_C06) from spp. in Yunnan, China, and obtained their complete genome sequences via next-generation sequencing.

Molecular detection and phylogenetic analysis of lumpy skin disease virus in Yunnan Province, China from 2019 to 2023.

The lumpy skin disease (LSD), caused by the lumpy skin disease virus (LSDV), represents an emerging infectious disease that poses substantial economic losses to the cattle industries in China. This study aimed to investigate the epidemiological characteristics of LSDV in Yunnan Province, Southwest China, from 2019 to 2023. A Taqman-probe-based real-time PCR (qPCR) assay was developed for the molecular detection of LSDV nucleotides.

Isolation and Identification of Arboviruses from Mosquitoes in Yuxi Region of Yunnan, China.

Arboviruses, transmitted to humans and animals by blood-feeding arthropods, pose significant public health risks. This study investigates the types, distribution, and epidemiology of arboviruses in the central Yuxi region of Yunnan Province, providing crucial data for the prevention and control of mosquito-borne diseases. In 2015, mosquitoes were collected from Tonghai County, Huaning County, Jiangchuan District, and Chengjiang County in Yuxi using light traps.

Colorimetric reverse transcription loop-mediated isothermal amplification assay for visual, sensitive, and specific detection of enterovirus G.

Background: Enterovirus G (EV-G) is widely prevalent in pigs worldwide, and co-infections of EV-G and other diarrhea-causing pathogens have been reported in many countries, threatening the pig farming industry. There are some methods available for EV-G detection; however, the RT-LAMP method for detecting EV-G has not yet been developed. The aim of thisstudy was to establish a highly sensitive and visual RT-LAMP assay for the detection of EV-G.

PCV2 Infection Upregulates SOCS3 Expression to Facilitate Viral Replication in PK-15 Cells.

Porcine circovirus type 2 (PCV2) is a globally prevalent swine pathogen that induces immunosuppression, predisposing pigs to subclinical infections. In intensive farming systems, PCV2 persistently impairs growth performance and vaccine efficacy, leading to substantial economic losses in the swine industry. Emerging evidence suggests that certain viruses exploit Suppressor of Cytokine Signaling 3 (SOCS3), a key immune checkpoint protein, to subvert host innate immunity by suppressing cytokine signaling.

Diversity and Seasonal Abundance of (Diptera: Ceratopogonidae) in Tengchong County of Yunnan, China.

(Diptera, Ceratopogonidae) are small biting midges and are known as vectors for many arboviruses, including bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV). Tengchong County of Yunnan Province, China, which borders Myanmar, has many private farms with goats, sheep, and cattle. To estimate the risk of -borne viral diseases such as bluetongue (BT) and epizootic hemorrhagic disease (EHD) in this area, an investigation of the diversity and abundance of in Tengchong between May 2024 and April 2025 was performed.

Whole-Genome Sequencing and Structure Study of Three Biting-Insect-Associated Viruses (, Guangxi Orbivirus, and Yongshan Totivirus) Isolated in Yunnan, China.

Yunnan Province is an area in China with a major prevalence of biting arthropods (including mosquitos, ticks, and ) and arboviruses including dengue virus (DENV), bluetongue virus (BTV), and epizootic hemorrhagic disease virus (EHDV). Therefore, attempts to isolate and detect arboviruses are frequently conducted in Yunnan during the past decades. In this study, a total of three viral strains/isolates (LF6-4, LF3-1, and LF6C2) previously isolated from cattle, goat, and in Lufeng County, Yunnan Province, between 2022 and 2023 were used for whole-genome sequencing, phylogenetic analysis, and electron microscopic analysis.

The gut microbiota of pregnant and non-pregnant female Hipposideros pomona.

The Pomona roundleaf bats (Hipposideros pomona) have unique ecological and physiological adaptations to various habitats in South Asia, including China. Despite its ecological significance, the gut microbiota plays a key role in regulating host immunity and reproductive physiology, possibly connecting microbial dynamics to fertility outcomes. In this study, we used V3-V4 16 S rRNA high-throughput sequencing to elucidate the diverse microbiota structure of pregnant and non-pregnant female H.

Mitochondrial Genome Comparison and Phylogenetic Analysis of Four Species of Dung Beetles (Coleoptera: Scarabaeidae: Scarabaeinae).

Dung beetles, despite their well-documented ecological significance, remain surprisingly underrepresented in molecular biology research, with a notable paucity of genomic and molecular data available for this ecologically crucial insect group. We sequenced and analyzed the whole mitochondrial genomes of four species of dung beetles, including , , , and from the Scarabaeinae subfamily. The results showed that the mitochondrial genome sizes of the four dung beetle species ranged from 14,977 bp to 18,425 bp.

Isolation of four serotypes of epizootic hemorrhagic disease virus from spp. and their associated infections in cattle in Yunnan, China.

Unlabelled: Epizootic hemorrhagic disease (EHD) is a noncontagious viral infection transmitted by certain species of the genus and has been included on the World Organization for Animal Health (OIE) list of diseases affecting domestic and wild ruminants. This study isolated four serotypes of EHDV strains (EHDV-1, -5, -6, and -7) from three species collected in Yunnan Province, China. The phylogenetic analysis of Seg-3 revealed that EHDVs can be classified into two groups based on their geographical origins: Eastern and Western.

Research note: Effects of fermented vegetable extract supplementation via drinking water on growth performance, immune function, and antioxidant status in broiler chickens.

This study investigated the effects of supplementing drinking water with fermented vegetable extract on growth performance, immune response, antioxidant activity, and hematological parameters in broiler chickens. A total of 240 one-day-old Cobb-500 broiler chicks were randomly allocated into four treatment groups, each comprising three replicates of 20 birds. The experimental groups were as follows: Group A (control, no extract), Group B (0.

Dietary Protein-Induced Changes in Archaeal Compositional Dynamics, Methanogenic Pathways, and Antimicrobial Resistance Profiles in Lactating Sheep.

Dietary protein levels greatly influence gut microbial ecosystems; however, their effects on gut archaea and associated functions in ruminants require further elucidation. This study evaluated the impact of varying dietary protein levels on gut archaeal composition, antimicrobial resistance (AMR) genes, virulence factors, and functional capacities in sheep. Eighteen ewes (Yunnan semi-fine wool breed, uniparous, 2 years old, and averaging 50 ± 2 kg body weight) were randomly assigned to diets containing an 8.

Influence of dietary protein levels on metabolism of nitrogen, phosphorus, and calcium in a Chinese indigenous sheep (Ovis aries).

Protein balance in ruminants' dietary regimens is crucial since it influences not just the production, fertility, and health of these animals, but also reduces worldwide environmental concerns regarding methane emissions. The current experiment explored the influence of dietary protein levels on nitrogen, calcium, and phosphorus metabolism and their environmental excretion using the Yunnan semi-fine wool sheep (YSFW) as the animal model. For this, 25 YSFW sheep (male, 10-month-old, averaging live body weight 38.

Hindgut microbiota profiling and functional prediction of sheep (Ovis aries) fed with different dietary calcium levels.

Hindgut microbiota plays a crucial role in small ruminant health and productivity, with diet being a key influencing factor. This study aimed to investigate the hindgut microbiota profile and functional prediction in sheep fed with different dietary calcium levels. Thirty male Yunnan Semi-Fine Wool rams (10 months old, 40.

Antiviral activity of nitazoxanide against pseudorabies virus infection .

Pseudorabies (PR), an infectious disease caused by pseudorabies virus (PRV), has been responsible for substantial economic losses within the global swine industry. However, effective control measures and vaccines against PRV remain limited, thereby underscoring the necessity for the development of innovative antiviral agents targeting PRV. Nitazoxanide (NTZ) is an FDA-approved anthelminthic drug, has shown efficacy in inhibiting a variety of viral infections.

Genetic Evaluation of Early Growth Traits in Yunnan Semi-Fine Wool Sheep.

With economic development and improved living standards, the demand for mutton and wool continues to grow, and improving the production performance and genetic potential of sheep breeds has become the key to promoting the high-quality development of the sheep industry. Thus, this study analyzes the influencing factors of the early production traits of Yunnan semi-fine wool sheep, optimizes the genetic evaluation model, and relies on accurate genetic parameter estimation to provide a theoretical basis for formulating a scientific and efficient breeding strategy for this breed. Data were collected from the Laishishan and Xiaohai breeding farms in Qiaojia, Yunnan, covering production records of the core flock from 2018 to 2022.

Mucosal barrier function and microbial community of small intestines in sheep in response to dietary energy concentrations.

The intestinal barrier and local immune response in ruminants are closely related to dietary energy concentration. To determine the optimal dietary energy concentration for the intestinal health of sheep, we compared microbiota, barrier function, and mucosal immunity in the small intestine of sheep consuming diets with different metabolisable energy (ME) concentrations. Forty-five growing Yunnan semi-fine wool sheep, aged 10 months and weighing 30.

Effects of semen collection methods on sperm quality and metabolite profile in goat seminal plasma: Comparing between artificial vagina and electro-ejaculator techniques.

This study compared two semen collection methods-artificial vagina (AV) and electro-ejaculator (EE)-to evaluate their effects on sperm quality and the metabolomic profile of goat seminal plasma (SP). Semen samples were analyzed for sperm characteristics, and SP was assessed using ultra-performance liquid chromatography-electrospray ionization quadrupole Orbitrap mass spectrometry. Sperm collected via AV showed lower ejaculate volume but significantly higher concentration and motility than EE.

Nasibacterium caprae gen. nov., sp. nov., isolated from a goat with respiratory disease.

A Gram-stain-negative, aerobic and rod-shaped bacteria, designated ZY210820, was isolated from the nasal cavity of a goat with respiratory disease in Yunnan Province, PR China. The strain grew at pH 6.0-9.

Dietary Supplementation of as Probiotic Influenced the Growth Performance, Hematological Parameters, Immune Function, Antioxidant Status, and Digestive Enzyme Activity of Nile Tilapia Fingerlings ().

This study evaluated the impact of supplementation on growth, antioxidant status, digestive enzyme activities, hematological parameters, blood biochemistry, and immune responses in Nile tilapia () fingerlings. A total of 180 fingerlings (mean weight: 5 ± 0.5 g) were randomly assigned to four groups: a control group and three probiotic-supplemented groups with 10 (S-1), 10 (S-2), and 10 (S-3) CFU g of , following a completely randomized design.

First Isolation and Characterization of Three Strains of Porcine Sapelovirus in Yunnan Province, China.

In order to investigate the causes of swine diarrhea in Yunnan Province, this study was conducted to detect and monitor diarrhea viruses through regular sampling and reverse transcription polymerase chain reaction (RT-PCR). In October 2023, porcine sapelovirus (PSV) was detected in fecal specimens collected from diarrheal pigs in Honghe City, and three strains of PSV were successfully isolated by inoculating them into PK-15 cells; electron microscopy revealed virus particles with diameters of ~32 nm. Next-generation sequencing (NGS) revealed that the PSV isolate genomes ranged from 7480 to 7515 nucleotides in length.

Diversity of Hepatitis E Viruses in Rats in Yunnan Province and the Inner Mongolia Autonomous Region of China.

Hepatitis E virus (HEV) is one of the most common pathogens causing acute hepatitis. Rat HEV, a member of the genus , infects mainly rat but can also cause human zoonotic infection. A survey of the virome of rats via next-generation sequencing (NGS) was performed in Yunnan Province and Inner Mongolia in China.

Genomic Characterization and Phylogenetic Analysis of Five Avian Influenza H5N1 Subtypes from Wild in Yunnan, China.

Highly pathogenic avian influenza (HPAI) H5 viruses have been found to have a substantial geographic distribution since they were first reported in Guangdong Province, China. The emergence of new genotypes threatens the poultry industry and human health worldwide. Here, we report five HPAI H5N1 variants isolated from in Yunnan Province, China.

Isolation and biological characterization of a variant pseudorabies virus strain from goats in Yunnan Province, China.

Pseudorabies virus (PRV), the causative agent of Aujeszky's disease, is an infectious pathogen that significantly impacts the global swine industry. The broad host range of PRV enables it to infect various animals, including pigs, cattle, minks, dogs, and even humans. Although PRV infections in ruminants have been reported, the occurrence of natural PRV infection in goats has not been documented.