Viscoelastic characterization of the human osteosarcoma cancer cell line MG-63 using a fractional-order zener model through automated algorithm design and configuration.

Understanding the viscoelastic properties of cells is essential for studying their mechanical behavior and identifying disease-related biomechanical markers. This paper proposes an integrated framework that combines fractional modeling with automated algorithm design to fit force-relaxation data acquired through atomic force microscopy. We employ the fractional-order zener model to describe cell relaxation curves and formulate the parameter estimation as a black-box optimization problem.

Synthesis of Silver Nanoparticles from Bitter Melon () Extracts and Their Antibacterial Effect.

We utilized silver nanoparticles synthesized from bitter melon () extracts for testing against the common agricultural pathogen . The synthesized nanoparticles were characterized and confirmed as silver nanoparticles by using ultraviolet spectroscopy, Fourier transform infrared spectroscopy, and scanning electron microscopy analysis. The results show that AgNPs were effective against ATCC25922 strain.

New Tool Against Tuberculosis: The Potential of the LAMP Lateral Flow Assay in Resource-Limited Settings.

Tuberculosis (TB) is a global public health issue requiring early and accurate diagnosis. The loop-mediated isothermal amplification (LAMP) assay is a promising alternative recommended by the WHO for the initial diagnosis of pulmonary TB, particularly in resource-limited settings. This study evaluated the sensitivity and specificity of a commercial LAMP assay for TB detection using 198 samples from different countries including Mexico.

Allelic and Genotypic Distribution of -77 A/G Polymorphism in Salvadoran Children With and Without Caries.

: Polymorphisms in metalloproteinases (MMPs) have the potential to be probable genetic biomarkers of dental caries. The aim of this study was to evaluate the distribution of the A/G rs2252070 single nucleotide polymorphism (SNP) according to caries experience in Salvadoran children. : A cross-sectional study was carried out on 185 Salvadorian school children between 7 and 12 years of age.

Arthritis Mutilans: A Devastating Manifestation of Psoriatic Disease Without Dactylitis in a 61-Year-Old Patient.

Arthritis mutilans (AM) is the most severe and least common form of psoriatic arthritis (PsA), characterized by digital shortening due to osteolysis and often associated with profound functional disability. We report a case of a 61-year-old male with longstanding plaque psoriasis and poor treatment adherence who developed AM affecting the interphalangeal joints of the left foot, with significant joint deformity and extensive skin involvement (30% body surface area). Imaging revealed severe bone breakdown, leading to the affected phalanges of the foot appearing shortened or telescoped.

Assessment of POPs and Remediation Proposal at a Former Pesticide Plant in Salamanca, Mexico.

In Salamanca, Mexico, a point source of Persistent Organic Pollutants (POPs) has been identified in an area previously occupied by the Tekchem industrial plant, which was formerly dedicated to the production and marketing of agrochemicals. This study aimed to develop a strategy to reduce the concentration of 13 POPs found in soil. The compounds studied were: α-hexachlorocyclohexane (α-HCH), β-hexachlorocyclohexane (β-HCH), γ-hexachlorocyclohexane (γ-HCH), heptachlor, heptachlor epoxide, aldrin, dieldrin, endrin, 2,4'-dichloro diphenyl dichloroethane (2,4'DDD), 4,4'-dichlorodiphenyl dichloroethane (4,4'DDD), 4,4'-dichlorodiphenyl dichloroethylene (4,4'DDE), 2,4'-dichlorodiphenyl trichloroethane (2,4'DDT), and 4,4'-dichlorodiphenyl trichloroethane (4,4'DDT).

Physical Activity and Psychological Symptoms in University Teachers Improved Post-COVID-19 Lockdown, but Sedentary Behavior Persisted.

: This study aimed to determine whether the movement patterns and mental health of university teachers changed after returning to on-site class activities following the COVID-19 lockdown. Specifically, it compared levels of physical activity (PA), sedentary behavior time (SBT), active breaks (ABs), and symptoms of depression, anxiety, and stress among university teachers during online and on-site teaching periods. We also analyzed the association between movement patterns with psychological and anthropometric variables.

Characterisation and Hypolipidaemic Effects of Tlayudas, Widely Consumed Tortillas, Containing Extracts on an In Vivo Model of Hypercholesterolaemia.

Tlayudas, a variant of tortillas, are a highly consumed and low-cost traditional Mexican food, typical of the Oaxaca region. We developed new functional food products from tlayudas (T) containing standardised hydroalcoholic extracts (T + -1 and T + -2) of the medicinal mushroom () from Mexico. These products were characterised through physicochemical and sensory properties, analysis of bioactive compounds and hypolipidaemic capacity using an in vivo model (C57BL/6 mice) of hypercholesterolaemia.

Entropy Generation Analysis and Performance Comparison of a Solid Oxide Fuel Cell with an Embedded Porous Pipe Inside of a Mono-Block-Layer-Build Geometry and a Planar Geometry with Trapezoidal Baffles.

An analysis of entropy generation and a performance comparison are carried out for a solid oxide fuel cell with an embedded porous pipe in the air supply channel of a mono-block-layer-build geometry (MOLB-PPA SOFC) and a planar geometry with trapezoidal baffles inside the fuel and air channels (P-TBFA SOFC). The results for power density at different current densities are discussed. Also, a comparison of the field of species concentration, temperature, and current density on the electrode-electrolyte interface is analyzed at a defined power density.

Real Space Partitioning of the First Hyperpolarizability.

Two approximate partitioning schemes for the principal component of the first static hyperpolarizability are introduced within the framework of the quantum theory of atoms in molecules (QTAIM). The first approach employs a two-state model, while the second utilizes finite difference techniques. Both methods decompose the principal hyperpolarizability component into contributions arising from atom polarizations and charge transfer.

Molecular mechanisms associated with the interaction of external electromagnetic fields in protein dynamics and aggregation: a focus on amyloid-peptide.

Transcranial stimulation has emerged as a non-invasive treatment that applies electrical currents and magnetic fields to regulate brain functions. Previous studies have shown that magnetic stimulation modulates the dynamics of charged molecules in biological systems. In some pathologies, once the electrical or magnetic field is applied directly to subjects, it can interact with, and alter, abnormally folded proteins, including amyloid-peptides and their aggregates, reducing cognitive impairments.

Functional Head Tremor: Contrasting Features with Other Tremor Etiologies.

Background: Functional tremor (FT) is considered the most common phenomenology among patients with functional movement disorders (FMDs). Most patients have limb tremor, but they can also present with tremor involving the head and trunk.

Objectives And Methods: We aimed to assess the clinical phenomenology of functional head tremor (FHT) and contrast it with features of HT observed in 125 patients with other tremor etiologies (OTE), including 71 patients with essential tremor (ET).

Simple and Complex Phonic Tics in Tourette Syndrome.

Unlabelled: Tourette syndrome (TS) is the most common cause of tics. Tics are classified as motor and phonic tics. The latter (previously also referred to as "vocal tics") are manifested by simple sounds (simple phonic tics) or complex, often semantically meaningful utterances (complex phonic tics).

Enhancing Legged Robot Locomotion Through Smooth Transitions Using Spiking Central Pattern Generators.

In this work, we propose the integration of a mechanism to enable smooth transitions between different locomotion patterns in a hexapod robot. Specifically, we utilize a spiking neural network (SNN) functioning as a Central Pattern Generator (CPG) to generate three distinct locomotion patterns, or gaits: walk, jog, and run. This network produces coordinated spike trains, mimicking those generated in the brain, which are translated into synchronized robot movements via PWM signals.

Tremor and Ataxia in Paraneoplastic Anti-Diacylglycerol Lipase Alpha (DAGLA) Cerebellitis.

Antibodies directed against the enzyme diacylglycerol lipase alpha (DAGLA) have been recently discovered to cause a severe autoimmune cerebellar syndrome. We report a patient with DAGLA antibodies with prominent tremor and ataxia occurring in the context of a malignant melanoma, indicating that these antibodies may also occur in paraneoplastic cerebellar degeneration.

Steepest-Entropy-Ascent Framework for Predicting Arsenic Adsorption on Graphene Oxide Surfaces: A Case Study.

Water contamination by arsenic(V) constitutes a major public-health concern, underscoring the need for models that capture both equilibrium and transient adsorption behavior. A framework that can do so is the steepest-entropy-ascent quantum thermodynamic (SEAQT) framework, which is used here to describe the uptake of As(V) on graphene oxide (GO) across pollutant concentrations of 25-350 mg L. A nonequilibrium equation of motion derived from the steepest-entropy-ascent principle for a five-component system (water, arsenic, two GO functional groups, and H) is solved with an energy eigenstructure generated by a Replica-Exchange Wang-Landau algorithm and then extrapolated to relevant contaminant concentrations via an artificial neural network.

Insulin resistance in cancer risk and prognosis.

Obesity-associated insulin resistance (IR) is characterized by a chronic low-grade inflammatory state driven by adipocyte dysfunction, elevated free fatty acids, and proinflammatory cytokines. These factors activate signaling pathways-including JNK, TLR, and NF-κB-that impair insulin receptor function and promote hyperinsulinemia. Elevated insulin reduces insulin-like growth factor binding proteins, thereby increasing the bioavailability of IGF-1 and IGF-2, which stimulate oncogenic pathways such as PI3K-AKT-mTOR and RAS-MAPK.

Exploring thurincin H as a biotechnologically relevant sactipeptide after fifteen years of research.

Thurincin H is an anionic antimicrobial peptide of 31 amino acids with a molecular weight of 3139.51 Da, it belongs to the group of sactipeptides and is produced by the bacterium Bacillus thuringiensis. Its antimicrobial spectrum is mainly directed against Gram-positive bacteria, and it has been shown to have antimicrobial activity against food pathogens and phytopathogens.

Assessment of arsenite removal efficiency, resistance, and biotransformation by Microbacterium hydroxycarbonoxydans isolated from contaminated sites.

There are reports on resistance to metals by the Microbacteriaceae family, although few studies have focused on the Microbacterium genus. The present work is one of the first studies related to arsenic (As) resistance and removal by Microbacterium hydrocarbonoxydans. Growth curves were performed simultaneously as follows: (1) growth kinetics without As, and (2) growth kinetics added with As(III).

Cognitive Stimulation and Its Effects on Well-Being, Executive Functions, and Brain-Derived Neurotrophic Factor in Older Adults from a Mexican Geriatric Center: A Quasi-Experimental Study.

The progressive increase in the aging population highlights the need for interventions aimed at preserving cognitive health and overall well-being in older adults. This study aimed to assess the impact of a structured cognitive training program on psychological well-being, executive function performance, and Brain-Derived Neurotrophic Factor levels (BDNF) in older adults from a Mexican geriatric center. A quasi-experimental pretest-posttest design with a nonequivalent control group was conducted.

Soluble Receptor of Advanced Glycation End Products and Cardiometabolic Markers in Children.

Background The soluble receptor for advanced glycation end products (sRAGE) is a proposed obesity biomarker, but its role in childhood has not been fully elucidated. This study aimed to evaluate the association between sRAGE levels and cardiometabolic factors in children according to body mass index (BMI). Introduction This cross-sectional study included 124 children aged 6-9 years, categorized as normal weight (n=72) and overweight or with obesity (n=52).

The functional significance of vascular DNA hypermethylation in atherosclerosis: a historical perspective.

A decade ago, independent mechanistic and descriptive epigenomics data demonstrated for the first time that vascular DNA hypermethylation is a landmark of and causal factor in human and murine atherosclerosis. Since then, a flurry of converging evidence has assigned a prominent role to vascular DNA hypermethylation across the natural history of cardiovascular disease (CVD), from the exposure to risk factors, to the onset and progression of the atheroma. DNA hypermethylation is induced by and mediates the metabolic outcomes of high-fat diets and CVD risk-enhancing lipids in several models.

Correlation Between COVID-19 Recovery, Executive Function Decline, and Emotional State.

Objective: The aim of this study was to determine whether there is a relationship between the time since recovery from coronavirus disease 2019 (COVID-19) and alterations in executive functions. We also evaluate the emotional state of post-COVID-19 patients.

Patients And Methods: We assessed patients between 18 and 50 years old, who had a history of COVID-19 with mild, moderate, or severe illness.

Germination and Outgrowth of Spores Deficient in BER and DisA Unveil Alternative Genetic Checkpoints.

During spore germination/outgrowth, the rehydration of the spore core and activation of aerobic metabolism can generate reactive oxygen species (ROS)-promoted DNA lesions that are repaired via the base excision repair pathway (BER). Accordingly, spores deficient in the AP-endonucleases (APEs) Nfo and ExoA exhibit a delayed outgrowth that is suppressed following disruption of the checkpoint protein DisA. Here, we report that DisA-independent DNA damage checkpoints operate during spore outgrowth.