Use of Electrolysed Water as Disinfection Technology in Front of Various Microorganisms and SARS-COV-2.

The global emergence of SARS-CoV-2 has highlighted the urgent need for effective disinfection strategies to mitigate virus transmission. Electrolyzed water (EW), an eco-friendly and cost-effective biocidal agent, has garnered attention for its broad-spectrum antimicrobial activity. This study evaluates the virucidal, bactericidal, and fungicidal capacities of EW with diverse pH, with a focus on its effectiveness against SARS-CoV-2 and other pathogens.

Physicochemical, Nutritional and Functional Properties of Neltuma affinis Pod Powders: a Novel Ingredient for Food Applications.

The pods of Neltuma species have been traditionally used as nutrient-rich sources of food ingredients. However, Neltuma affinis, a native species of South America, remains understudied. This work aimed to produce and characterize powders from different pod fractions (specifically, the endocarp-seeds (ESF) and epicarp-mesocarp (EMF) fractions) by dry grinding, evaluating their physicochemical, nutritional, functional, and cytotoxic properties.

A systematic review of health promoting effects of consumption of whey-based fermented products on adults.

Introduction: Fermented whey-based products show significant potential as functional foods, owing to their rich nutritional profile and the generation of bioactive compounds during fermentation. This systematic narrative review evaluates the health effects of fermented-whey consumption based on evidence from human studies in adults.

Methods: A systematic literature search was conducted using electronic databases including, PubMed, Scopus, and Cochrane Library for studies published between 1.

Generative Artificial Intelligence and the Craft of Qualitative Health Research: Observations From a Techno-Negative Stance.

In this essay, I offer my take on contemporary matters relevant to the existing, emerging, and imagined intersections between qualitative health research (QHR) and generative artificial intelligence (GenAI). The essay's central argument is that the increasing reliance on GenAI in QHR is eroding scholarly craftspersonship and should be challenged. In order to present and justify this argument, I posit five coordinated observations: The growing body of literature on using GenAI in qualitative research is reducing qualitative research to coding and pattern recognition; the turn to GenAI disincentivizes reading and stultifies qualitative health researchers; the infatuation with GenAI amplifies the process of McDonaldization of QHR; the time that GenAI saves us isn't being used to become better researchers; and our tendency to humanize GenAI may dehumanise us, whereas craftspersonship is a state of being human.

Organ-level gene regulatory networks inferred from transcriptomic data reveal context-specific regulation and highlight novel regulators of ripening and ABA-mediated responses in tomato.

Tomato (Solanum lycopersicum) is a globally important crop, yet the gene regulatory networks (GRNs) controlling gene expression remain poorly understood. In this study, we constructed GRNs for roots, leaves, flowers, fruits, and seeds by inferring transcription factor (TF)-target interactions from over 10,000 RNA-seq libraries using the GENIE3 algorithm. We refined these networks with gene co-expression data and computational predictions of TF binding sites.

Defining non-invasive criteria for indicating drug therapy in metabolic-associated steatotic liver disease in clinical practice.

Background: &Aim:Resmetirom is the first FDA-approved drug for metabolic-associated liver disease(MASLD) in F2-F3 patients with steatohepatitis. Non-invasive criteria have been proposed for initiating treatment; however, these have not been validated in clinical practice. We validated the proposed criteria and established new guidelines for initiating resmetirom treatment in clinical practice.

Description of chromatographic performance in reversed-phase liquid chromatography based on a generalised model. Part II: Prediction of serial-column coupling.

While reversed-phase liquid chromatography (RPLC) using C18 columns remains a cornerstone technique in analytical separations, its limited selectivity has driven the development of alternative stationary phases and advanced strategies, such as serial-column coupling, to expand chromatographic capabilities. This approach connects columns of different lengths and chemistries in series to create hybrid configurations that enhance separation performance. Beyond improving resolution, serial coupling can also reduce analysis times, particularly combined with modern zero-dead-volume connectors that minimise band broadening, which have renewed interest in this approach.

The evolutionary history of insect-associated xinmoviruses.

Viruses are ubiquitous biological entities that shape host evolution and ecosystem dynamics. Among them, negative-sense single-stranded RNA (-ssRNA) viruses form a diverse group that includes well-characterized vertebrate pathogens and an expanding repertoire of invertebrate-infecting viruses. Recent advances in high-throughput sequencing have revealed numerous insect-associated -ssRNA viruses, reshaping our understanding of their diversity, transmission, and ecological significance.

What we cannot miss in clinical trials on compression therapy.

BackgroundCompression therapy (CT) is widely prescribed for chronic venous disease (CVD), lymphedema, and lipedema despite robust evidence supporting its effectiveness only in a minority of clinical scenarios.AimProvide an overview of the methodological parameters essential for obtaining a high-quality scientific trial.MethodsThe International Compression Club (ICC) chairman prepared a preliminary list of the main methodological flaws in publications on CT, and a list of parameters to be included in high-quality randomized controlled trials (RCTs).

Robust deep MRI contrast synthesis using a prior-based and task-oriented 3D network.

Magnetic resonance imaging (MRI) is one of the most widely used tools for clinical diagnosis. Depending on the acquisition parameters, different image contrasts can be obtained, providing complementary information about the patient's anatomy and potential pathological findings. However, multiplying such acquisitions requires more time, additional resources, and increases patient discomfort.

High-fidelity Cas9-mediated targeting of KRAS driver mutations restrains lung cancer in preclinical models.

Missense mutations in the 12 codon of KRAS are key drivers of lung cancer, with glycine-to-cysteine (G12C) and glycine-to-aspartic acid (G12D) substitutions being among the most prevalent. These mutations are strongly associated with poor survival outcomes. Given the critical role of KRAS in lung cancer and other cancers, it remains as a major target for the development of new and complementary treatments.

ESP-INOCA: Rationale and design of a national multicentre registry for prognostic stratification in patients with suspected myocardial ischaemia and non-obstructive coronary arteries.

Introduction: Obstructive coronary artery disease (CAD) has long been recognized as the primary mechanism underlying myocardial ischaemia. However, up to 50 % of patients with angina and objective evidence of ischaemia present with non-obstructed coronary arteries (INOCA) in the coronary angiography, falling under the umbrella term INOCA, a heterogeneous group that remains frequently underdiagnosed and undertreated. Performing a coronary function test (CFT) enables us to diagnose and stratify these patients into different endotypes and start a targeted medical therapy.

Retrograde Vital Pulp Treatment in External Root Resorption Due to Third Molar Impaction: A Proof-of-Concept and Case Report.

: Third molar impaction with the consequent root resorption of second molars often creates complexities in treatment planning and execution. In the past, the root canal treatment (RCT) of second molars was required in these cases to avoid pulp necrosis and infection. The aim of this paper is to report a surgical/retrograde approach for the maintenance of pulp vitality, proposed as retrograde vital pulp treatment (rVPT), in cases of asymptomatic or reversibly affected teeth with root resorptions caused by impacted adjacent teeth.

Validation of pharmacokinetic model for quizartinib quantified by UPLC-MS/MS in patients with FLT3-ITD negative newly diagnosed acute myeloid leukemia.

Purpose: Quizartinib pharmacokinetics in FLT3-ITD negative acute myeloid leukemia (AML) remain largely unexplored. This study aims to validate a population pharmacokinetics model (popPK) for quizartinib in plasma samples of FLT3-ITD negative AML patients. To do so, an ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) method has been developed and validated for the quantification of quizartinib.

Mesoporous silica nanoparticles with an azobenzene gatekeeper as hypoxia-responsive nanocarriers for targeted doxorubicin delivery.

Hypoxia is a key feature of solid tumors, contributing to therapeutic resistance and poor prognosis. Targeting hypoxic environments presents an opportunity to enhance drug delivery selectivity and improve treatment outcomes. Among nanocarriers, mesoporous silica nanoparticles (MSNs) have demonstrated great potential for drug delivery; however, achieving precise control over drug release remains a challenge.

Quantum metrology with a continuous-variable system.

As one of the main pillars of quantum technologies, quantum metrology aims to improve measurement precision using techniques from quantum information. The two main strategies to achieve this are the preparation of nonclassical states and the design of optimized measurement observables. We discuss precision limits and optimal strategies in quantum metrology and sensing with a single mode of quantum continuous variables.

Short-term association between hot nights and mortality: a multicountry analysis in 178 locations considering hourly ambient temperature.

Background: The rise in hot nights over recent decades and projections of further increases due to climate change underscores the critical need to understand their impact. This knowledge is essential for shaping public health strategies and guiding adaptation efforts. Despite their significance, research on the implications of hot nights remains limited.

CRISPR/Cas9-Driven Engineering of AcMNPV Using Dual gRNA for Optimized Recombinant Protein Production.

The CRISPR/Cas9 system is a powerful genome-editing tool that is applied in baculovirus engineering. In this study, we present the first report of the AcMNPV genome deletions for bioproduction purposes, using a dual single-guide RNA (sgRNA) CRISPR/Cas9 approach. We used this method to remove nonessential genes for the budded virus and boost recombinant protein yields when applied as BEVS.

Impact of Sarcopenic Diabetes on Outcomes and Mortality in Older Adults Hospitalized for Hip Fracture: A Nested Case-Control Study Within a Real-World Evidence Cohort.

Background: This study addresses the prevalence and outcomes of sarcopenic diabetes among older adults hospitalized for hip fractures, highlighting its association with nutritional status, frailty, in-hospital outcomes, and mortality. With the global incidence of hip fractures anticipated to rise significantly, understanding these associations is crucial, especially considering the higher prevalence of sarcopenia in diabetic patients, which exacerbates outcomes.

Methods: An observational, unicentric case-control study nested within a real-world data cohort was conducted.

Identification and Quantification of Pteridines in the Wild Type and the Mutant of (Hemiptera: Anthocoridae).

Nymphs of the mutant of (Fieber) are orange-colored instead of the yellowish color of the wild-type individuals. Since there were no previous studies of the pigments of this species, we searched for differences in pigments of the pteridine family between both strains. Fluorescent compounds from nymph extracts were separated by cellulose thin-layer chromatography (TLC) and by size exclusion chromatography, followed by LC/MS/MS.

Connected by Boredom: A Systematic Review of the Role of Trait Boredom in Problematic Technology Use.

In an increasingly pervasive digital environment, trait boredom has been identified as a key psychological factor in the onset and maintenance of problematic digital technology use. This systematic review aims to investigate the role of trait boredom in digital behavioral addictions, including problematic smartphone use, Internet and social media overuse, and gaming addiction, through theoretical models such as the I-PACE model and the Compensatory Internet Use Theory (CIUT). A systematic literature search was conducted across multiple scientific databases (PsycINFO, Web of Science, PubMed, and Scopus), yielding a total of 4603 records.

Prevalence and Risk Assessment of Multiple Mycotoxins in Durum Wheat from Fields Under Different Agricultural Practices in Tunisia.

Mycotoxin contamination in wheat, a staple food critical to human nutrition, poses significant public health concerns. This study investigated the natural occurrence of 17 mycotoxins in Tunisian durum wheat, assessed the influence of soil tillage practices on mycotoxin contamination, and performed an associated exposure risk assessment. A total of 167 wheat samples were randomly collected over two years (2021 and 2022) from fields managed under conventional tillage (CT) and no-tillage (NT) systems during both pre- and post-harvest periods.

Exploring anaerobic fermentation stability against a temperature perturbation: process indicators and recovery strategies.

Anaerobic fermentation (AF) efficiency is highly influenced by temperature, and operational disruptions in industrial settings can lead to severe process failure. In this study, AF of food wastes (FWs) was subjected to a temperature drop from 25 °C to 15 °C to elucidate AF behaviour and proper recovery strategies. The temperature decrease caused a marked decline in volatile fatty acids (VFAs) production from 23.

Unveiling Spin Selective Mechanism toward Very Large Spin Signals in Black Phosphorus 2D Semiconductor Spintronics Devices.

Discovering an efficient spintronic semiconductor workhorse with dual host capabilities as a channel and spin valve barrier remains one of the most elusive endeavors toward the development of spin-logic circuits. Graphene paved the way for two-dimensional (2D) materials, yet engineering a controlled band gap in it remains a challenge. Black phosphorus (BP) was recently unveiled as a potential candidate in the realm of 2D semiconductors, with carrier mobilities among the largest reported for a 2D material and a low spin-orbit coupling reminiscent of graphene.

The protective effect of flower against mycotoxin toxicity in a gastrointestinal barrier model.

Objective: We investigated the phytochemical composition, gastrointestinal bioaccessibility, and protective effects against mycotoxin-induced toxicity of L. flower, a botanical species traditionally used in European herbal medicine.

Methods: flower samples were collected from the Majella National Park and chemically characterized using HPLC-PDA and NMR metabolomics, revealing the presence of abundant bioactive compounds (iridoids, secoiridoids, and xanthones), as well as quantifying the levels of amino acids, organic acids, and sugars.