Tailoring of agarose hydrogel to modulate its 3D bioprintability and mechanical properties for stem cell mediated bone tissue engineering.

The intense gelling characteristics and viscosity constraints of agarose limit its utility as a sole ink material in 3D printing. This study presents the development of agarose bioink designed for cell-laden printing, featuring controlled printability, exceptional stiffness, and cell-responsiveness achieved via the insertion of photochemically reactive methacrylate groups. This chemical modification transforms the dense agarose network into a thinner structure, effecting a gentle thermogelling property that enhances the printability and facile cell encapsulation.

Early 2022 breakthrough infection sera from India target the conserved cryptic class 5 epitope to counteract immune escape by SARS-CoV-2 variants.

Unlabelled: During the coronavirus disease 2019 (COVID-19) pandemic, the vast majority of epitope mapping studies have focused on sera from mRNA-vaccinated populations from high-income countries. In contrast, here, we report an analysis of 164 serum samples isolated from patients with breakthrough infection in India during early 2022 who received two doses of the ChAdOx viral vector vaccine. Sera were screened for neutralization breadth against wild-type (WT), Kappa, Delta, and Omicron BA.

Exercise Delays Brain Ageing Through Muscle-Brain Crosstalk.

Ageing is often accompanied by cognitive decline and an increased risk of dementia. Exercise is a powerful tool for slowing brain ageing and enhancing cognitive function, as well as alleviating depression, improving sleep, and promoting overall well-being. The connection between exercise and healthy brain ageing is particularly intriguing, with exercise-induced pathways playing key roles.

Priestia and Phytobacter sp. prevent membrane damage and electrolyte leakage from Capsicum annuum L. seeds subjected to sub-optimal temperature stress.

Unlike the Himalayas, the sub-Himalayan zones did not experience snowfall and thus suitable for growing solanaceous vegetables. However, several cold waves have been reported to affect the district of Coochbehar (West Bengal, India), which belongs to the Cwa zone (as per Koppen's classification). Variable duration of sub-optimal soil temperature can have a detrimental effect on the growth of seedlings.

BMWP: the first Bengali math word problems dataset for operation prediction and solving.

Solving math word problems of varying complexities is one of the most challenging and exciting research questions in artificial intelligence (AI), particularly in natural language processing (NLP) and machine learning (ML). Foundational language models such as GPT must be evaluated for intelligence, and solving word problems is a key method for this assessment. These problems become especially difficult when presented in low-resource regional languages such as Bengali.

Detection of Oral Submucous Fibrosis at the Crossroads of Altering Nucleogeometry, Nuclear Spatial Distribution and Nuclear Molecular Abundance.

The manuscript concentrates on spatial distribution and various nucleomorphometric parameters of epithelium to diagnose Oral Submucous Fibrosis (OSF). Histologically confirmed OSF and normal submucosa tissue samples were procured and stained with diamidino phenylindole (DAPI) to visualize nuclei. E-cadherins and p63 were also immunohistochemically stained.

Fabrication of Folic Acid-Derived Carbon Dot-Conjugated Chitosan Nanospheres as Theragnostic Agents for pH-Responsive Anticancer Drug Delivery.

The favorable success rate in cancer treatment predominantly depends on precise diagnosis with target-specific drug delivery, which can regulate the patient survival outcome rate. Moreover, proper tracking of the system's pH is very much crucial as most of the therapeutic's action and release rate depend on it. Therefore, this work has been intended to fabricate a folic acid-derived carbon dot (FACD) decorated with chitosan (Cs) in order to form nanospheres (FACD-Cs-Ns) for anticancer doxorubicin hydrochloride (Dox.

Keratin Rich PCL Blended Nano-Microfibrous Sheet as a Bioactive Immunomodulatory ECM Analog Toward Dermal Wound Healing-In Vitro and In Vivo Responses.

Being an excretory scleroprotein, human hair-derived keratin with inherent bioactive peptide cues may actively participate in an immunomodulatory role in the wound microenvironment. In the current study, nano-microfibrous structural attributes mimicking the extracellular matrix were prepared using a polymer blend containing a high loading of keratin as a bioactive matrix by electrospinning, where polycaprolactone (PCL) was used as an electrospinnable aid. The FESEM analysis showed smooth fibers with diameters ranging from 100 to 220 nm.

Nonenzymatic Electrochemical Sensor Device for On-Site Nitrate Determination Using Copper Anchored Magnetite Nanocomposites.

Elevated nitrate (NO) levels in potable water due to anthropogenic and geogenic activities pose a significant environmental threat. Herein, we report a highly efficient electrochemical sensor device for NO detection using a copper-anchored magnetite (Cu@FeO) nanocomposite. The electrochemical performance of the NO sensor was highly durable and reliable on a glassy carbon electrode (GCE) and as a proof of concept, it has been translated to thermal vapor deposited gold electrodes (Au electrodes) chip integrated with a hand-held portable potentiostat connected to a smartphone with read out capabilities for onsite real field application.

CoO/fluoro-copolymer nanocomposite modified boron-doped diamond electrode non-enzymatic sensor for the determination of skeletal muscle relaxant drug cyclobenzaprine in biological fluids.

Electrochemical sensors have revolutionized pharmaceutical analysis by providing enhanced speed, selectivity, and cost-effectiveness. This study presents the development of a highly sensitive, non-enzymatic electrochemical sensor for Cyclobenzaprine (CBZ) determination. The sensor features a boron-doped diamond electrode (BDDE) modified with a novel Cobalt Oxide/Nafion-based nanocomposite (Co₃O₄/Nafion), synthesized and optimized for superior performance.

Understanding the genetic epidemiology of hereditary breast cancer in India using whole genome data from 1029 healthy individuals.

Breast cancer is the most highly reported cancer in India. Genetic testing could help tackle the increasing cancer burden by enabling carriers obtain early diagnosis through increased surveillance, and help guide treatment. However, accurate interpretation of variant pathogenicity must be established in a population-specific manner to ensure effective use of genetic testing.

Patient-specific prediction of arterial wall elasticity using medical image-informed in-silico simulations.

Limitations in clinical cardiovascular research have driven the development of advanced simulations for patient-specific insights into arterial elasticity. However, uncertainties in model inputs, data resolution, and parameter estimation can compromise accuracy. Our research aimed to provide reliable estimates of the arterial wall elasticity non-invasively, where direct clinical measurement is difficult.

Simultaneous detection of trace protein biomarkers from a single drop of blood using AI-enhanced smartphone-based digital microscopy.

The detection of early-stage diseases is often impeded by the low concentrations of protein biomarkers, necessitating sophisticated and costly technologies. In response, we have developed an advanced cyber-physical system that integrates blood plasma separation, biomarker detection, and analysis on a single microfluidic platform. This novel system enables the simultaneous detection of Neuron-Specific Enolase (NSE) and Carcinoembryonic Antigen (CEA) with high specificity and accuracy.

Gallbladder cholangiocyte organoids.

Organoids are miniature three-dimensional (3D) organ-like structures developed from primary cells that closely mimic the key histological, functional, and molecular characteristics of their parent organs. These structures self-organize through cell-cell and cell-matrix interaction in culture. In the last decade, organoids and allied 3D culture technologies have catalyzed studies involving developmental biology, disease biology, high-throughput drug screening, personalized medicine, biomarker discovery, tissue engineering, and regenerative medicine.

Oxidative stress-driven enhanced iron production and scavenging through Ferroportin reorientation worsens anemia in antimony-resistant Leishmania donovani infection.

Despite the withdrawal of pentavalent-antimonials in treating Visceral leishmaniasis from India, recent clinical isolates of Leishmania donovani (LD) exhibit unresponsiveness towards pentavalent-antimony (LD-R). This antimony-unresponsiveness points towards a genetic adaptation that underpins LD-R's evolutionary persistence and dominance over sensitive counterparts (LD-S). This study highlights how LD evolutionarily tackled antimony exposure and gained increased potential of scavenging host-iron within its parasitophorous vacuoles (PV) to support its aggressive proliferation.

Functionalized amino acid-based injectable hydrogels for sustained drug delivery.

Drug delivery vehicles optimize therapeutic outcomes by enhancing drug efficacy, minimizing side effects, and providing controlled release. Injectable hydrogels supersede conventional ones in the field of drug delivery owing to their less invasive administration and improved targeting. However, they face challenges such as low biodegradability and biocompatibility, potentially compromising their effectiveness.

PSMC2 promotes resistance against temozolomide in glioblastoma via suppressing JNK-mediated autophagic cell death.

Temozolomide is universally used to treat glioblastoma due to its unique ability to cross the blood-brain barrier and inhibit tumor growth through DNA alkylation. However, over time, the inevitable emergence of resistance to temozolomide impedes successful treatment of this cancer. As a result, there is an urgent need to identify new therapeutic targets to improve treatment outcomes for this malignancy.

Skin-Integrated Electrogenetic Regulation of Vasculature for Accelerated Wound Healing.

Neo-vascularization plays a key role in achieving long-term viability of engineered cells contained in medical implants used in precision medicine. Moreover, strategies to promote neo-vascularization around medical implants may also be useful to promote the healing of deep wounds. In this context, a biocompatible, electroconductive borophene-poly(ε-caprolactone) (PCL) 3D platform is developed, which is called VOLT, to support designer cells engineered with a direct-current (DC) voltage-controlled gene circuit that drives secretion of vascular endothelial growth factor A (VEGFA).

Quantification of L-lactic acid in human plasma samples using Ni-based electrodes and machine learning approach.

This work presents a robust strategy for quantifying overlapping electrochemical signatures originating from complex mixtures and real human plasma samples using nickel-based electrochemical sensors and machine learning (ML). This strategy enables the detection of a panel of analytes without being limited by the selectivity of the transducer material and leaving accommodation of interference analysis to ML models. Here, we fabricated a non-enzymatic electrochemical sensor for L-lactic acid detection in complex mixtures and human plasma samples using nickel oxide (NiO) nanoparticle-modified glassy carbon electrodes (GCE).

Comparing Ag-O coordinated AgMOF-5 and Ag-N coordinated Ag nanosphere catalytic polymers for real time monitoring of HO level in cancer cells.

Monitoring HO levels in live cells is essential due to its superior stability and possible severity inside the cell. The quest for a superior platform capable of detecting cellular-level hydrogen peroxide (HO) concentrations without necessitating the use of high-cost enzymes is of utmost importance. Here, the quantification of intracellular HO concentrations has been performed using silver metal polymer-based nonenzymatic electrochemical detection.

New epigenome players in the regulation of PCSK9-H3K4me3 and H3K9ac alterations by statin in hypercholesterolemia.

Statins are the most effective drugs used worldwide to lower the serum LDL-C by inhibiting the rate-limiting step, HMG-CoA reductase, in cholesterol biosynthesis. Despite its prevalent use, statins are known to increase proprotein convertase subtilisin/kexin type 9 (PCSK9) expression, hindering its efficiency. However, the underlying mechanisms remain elusive.

Bone ingrowth in randomly distributed porous interbody cage during lumbar spinal fusion.

Porous interbody cages are often used in spinal fusion surgery since they allow bone ingrowth which facilitates long-term stability. However, the extent of bone ingrowth in and around porous interbody cages has scarcely been investigated. Moreover, tissue differentiation might not be similar around the superior and inferior cage-bone interfaces.

BRCAIndica: a resource for ACMG/AMP classified BRCA1 and BRCA2 variants.

As genetic testing becomes increasingly accessible and affordable, the uniform and accurate interpretation of genetic variants becomes essential. The ACMG/AMP joint guidelines provide the basis for systematic and uniform interpretation of pathogenicity of genetic variants. However, the application of these in routine clinical interpretation at-scale has largely been limited by the lack of resources providing harmonized data especially at a population-scale.